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Sampling device for virus nucleic acid detection

A virus nucleic acid and sampling device technology, applied in biochemical cleaning devices, enzymology/microbiology devices, specific-purpose bioreactors/fermentation tanks, etc. Sol, outbreak treatment and far away from the site, to achieve the effect of simple structure, easy to carry, and improve efficiency

Active Publication Date: 2020-09-01
马钰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, biosafety cabinets are mainly used for biosafety protection before specimens are added to pre-allocated plates. However, in many infectious disease emergencies, the outbreak treatment site is far away from the laboratory, or even from the urban area, or the local laboratory does not have a biosafety cabinet. These have brought difficulties to medical staff in quickly diagnosing the epidemic and on-site investigation of infected persons.
[0004] For highly contagious respiratory infectious diseases, such as COVID-19, the usual detection process is to use a cotton swab to collect exfoliated epithelial cells in the nasopharynx, put them into a sampling tube containing a virus preservation solution, and under the action of a vibrator, The cells on the surface of the cotton swab are shed into the solution, but this process is easy to generate a large amount of aerosol, and a large amount of aerosol particles containing the virus overflow when the cover is opened. The process of repeatedly drawing samples is also another aspect of contamination

Method used

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  • Sampling device for virus nucleic acid detection
  • Sampling device for virus nucleic acid detection
  • Sampling device for virus nucleic acid detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] like figure 1 A sampling device for viral nucleic acid detection shown, comprising a lower base 1, an upper base 2 arranged above the lower base 1 and moving along the X axis, and located above the X-axis movable base 2 along the Y axis A moving sample placement rack 3; the upper abutment can be carried out along the X-axis direction of the lower abutment 1, and the sample placement rack 3 can be moved along the Y-axis direction of the upper abutment, thereby ensuring that the sample placement rack Able to move in X-axis and Y-axis directions on the lower base 1;

[0038] Specifically, the upper surface of the lower base 1 is provided with an X-axis slide rail 5, and the upper base 2 is movably connected with the X-axis slide rail 5, and the upper base 2 is arranged on the upper base 2 and The X-axis driving device between the lower base 1 drives the upper base 2 to move along the X-axis direction; the X-axis slide rails 5 are two and parallel to each other, and the bo...

Embodiment 3

[0047] like image 3 The shown sampling tube includes a tube body 701, a constricted portion 702 provided at the bottom of the tube body 701, an upper sealing cover 705 fixedly connected to the upper part of the tube body 701, and the inside of the tube body 701 and the constricted portion 702. A serrated ring 704 with a serrated inner ring is fixed at the transition, and a rotary sealing assembly 703 for sealing the outlet of the constricted part 702 is fixed outside the constricted part 702 . The tube body 701 is made of a slightly soft material, which can ensure that the tube body is squeezed, and the mixture of cells and solutions collected in the tube body is dropped into the well groove of the 96-well plate of the kit in the sampling device , the aperture size of the small mouth of the narrow mouth 702 at the bottom of the necking portion 702 is 1mm, which can ensure that the area of ​​the droplet dripping out through the hole is 50ul. It is made of hard material to ens...

Embodiment 4

[0049] On the basis of Example 1, such as Figure 5 , Image 6 and Figure 7 The rotary seal assembly 703 includes an inner sleeve 7031 fixedly connected to the constriction portion 702, an outer sleeve 7032 connected to the inner sleeve 7031 with external threads, and the inner sleeve and the outer sleeve can be rotatably connected. The bottom of the outer sleeve 7032 is fixedly connected to the outer cylinder bottom cover 7033 with a circular through hole 7034 in the middle, and the lower part of the inner sleeve 7031 is provided with an inwardly extending inner neck end 7035; the inner sleeve 7031 It has an integrated structure with the constriction part 702, and the inner constriction end 7035 can ensure the mechanism of the entire rotary seal assembly 703 to be more stable. fluid dripping out of the body;

[0050] A first semicircle cover 7036 and a second semicircle cover 7037 are provided between the bottom of the inner neck end 7035 and the outer cylinder bottom cov...

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Abstract

The invention relates to a sampling device for virus nucleic acid detection. The sampling device comprises a lower base station, an upper base station, which is arranged above the lower base station and moves along an X axis, and a sample placing rack, which is arranged above the upper base station moving along the X axis and moves along a Y axis. An X-axis sliding rail is arranged on the upper surface of the lower base station, and the upper base station is movably connected with the X-axis sliding rail. A Y-axis sliding rail is arranged on the upper surface of the upper base station, and thesample placing rack is movably connected with the Y-axis sliding rail. A sealing cover is arranged on the lower base station. The structure is simple, and the sampling device is convenient to carry.When a sample that is biologically dangerous is treated, the sample is isolated by the sealing cover; the pollution of the sample to the environment or experiment operators is avoided; and the upper base station can move in the X-axis direction. The sample placing rack can move in the Y-axis direction. The sample placing rack can move in the X-axis direction and the Y-axis direction at the same time, it can be guaranteed that a sample collected in a sampling pipe can be accurately displaced when the sample placing rack moves, and the sampling efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of viral nucleic acid detection, in particular to a sampling device for viral nucleic acid detection. Background technique [0002] At present, nucleic acid detection methods are widely used in the detection of pathogenic microorganisms, and nucleic acid extraction is a key step in this detection method, especially for infectious diseases such as COVID-19. The experiment operator and the environment are very harmful, so it must be done in the biosafety cabinet of the P2 / P3 laboratory. [0003] At present, the nucleic acid extraction kits produced by most manufacturers have been pre-packed in 96-well plates. When using them, you only need to add the specimens to the lysate wells of the pre-packed plates for 5 minutes and wait for the virus to be completely inactivated before removing them from the safety cabinet. Take it out, and enter the lifting link on the machine. This subsequent step is no longer biolog...

Claims

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Application Information

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IPC IPC(8): C12M1/26C12M1/24C12M1/12C12M1/00B01L9/00
CPCC12Q1/24C12Q1/6806B01L9/00Y02A50/30
Inventor 马钰
Owner 马钰