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Fluorescence detection system, fluorescence biosensor and application thereof

A fluorescence detection and system technology, applied in fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problems of cumbersome detection steps, limited quantitative accuracy, and high detection cost, and achieve easy surface modification, low cost, and high signal-to-noise ratio Effect

Pending Publication Date: 2020-09-04
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Therefore, the technical problem to be solved by the present invention is to overcome the defects of high detection cost, cumbersome detection steps, and limited quantitative accuracy in the disease diagnosis of Alzheimer's disease in the prior art.

Method used

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  • Fluorescence detection system, fluorescence biosensor and application thereof
  • Fluorescence detection system, fluorescence biosensor and application thereof
  • Fluorescence detection system, fluorescence biosensor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This embodiment provides a fluorescence detection system, including the following components:

[0043] 1. The first fluorescent probe, the first fluorescent probe is a single-stranded DNA probe pDNA1 labeled with the fluorescent molecule 6-FAM at the 5' end, the nucleotide sequence of pDNA1 is shown in SEQ ID NO.1, the first fluorescent probe The needles recognize and bind miRNA1, a biomarker molecule for Alzheimer's disease.

[0044] 2. The second fluorescent probe, the second fluorescent probe is a single-stranded DNA probe pDNA2 labeled with a fluorescent molecule ROX at the 5' end, the nucleotide sequence of pDNA2 is shown in SEQ ID NO.3, the second fluorescent probe can Recognizes and binds miRNA2, a biomarker molecule for Alzheimer's disease.

[0045] 3. The third fluorescent probe, the third fluorescent probe is a single-stranded DNA probe pDNA3 labeled with a fluorescent molecule Cy5 at the 5' end, the nucleotide sequence of pDNA3 is shown in SEQ ID NO.3, the t...

Embodiment 2

[0050] This embodiment provides a fluorescent biosensor, including the fluorescence detection system in Embodiment 2. Fluorescent biosensors can realize the rapid detection of biomarker molecules of Alzheimer's disease, and have the advantages of fast, simple, sensitive, efficient and accurate, and can be used for disease screening of Alzheimer's disease with diagnosis.

experiment example 1

[0052] 1. Purpose of the experiment: To detect the specificity and sensitivity of the first fluorescent probe, the second fluorescent probe and the third fluorescent probe in Example 1 binding to the target biomarker molecule.

[0053] 2. Experimental process and results:

[0054] The fluorescence spectra of the three fluorescent probes were detected before incubation with graphene oxide, after incubation with graphene oxide, and after incubation with graphene oxide and miRNA, and the first fluorescent probe was recorded at 510nm-640nm band (excitation wavelength: 490nm), the fluorescence emission spectra of the first fluorescent probe in the 600nm-740nm band (excitation wavelength: 580nm) and the third fluorescent probe in the 670nm-790nm band (excitation wavelength: 640nm), the results are as follows figure 1 shown.

[0055] figure 1 A shows the change of the fluorescence emission spectrum of the first fluorescent probe pDNA1 under three conditions: in the absence of GO, p...

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Abstract

The invention discloses a fluorescence detection system, which comprises at least two fluorescence probes, wherein the at least two fluorescence probes combine with at least two biomarker molecules ofthe Alzheimer's disease, and the luminescence spectra of fluorescence molecules marked by the fluorescence probes are different from each other; and fluorescence quenching agents, wherein the fluorescence quenching agents adsorb the fluorescence probes, so that fluorescence of fluorescence molecules is quenched, and the fluorescence quenching agents and the fluorescence probes combined with the biomarker molecules are desorbed, so that the fluorescence of the fluorescence molecules is recovered. When the fluorescence detection system is used for detecting the biomarker molecules of the Alzheimer's disease, rapid detection of the biomarker molecules of the Alzheimer's disease can be achieved only by adding the fluorescence detection system to the target detection substance without using the large-scale instrument or carrying out the complex reaction steps, and the system has advantages of rapidness, simpleness, sensitivity and high efficiency. The invention further discloses a fluorescence biosensor which comprises the fluorescence detection system and can provide effective information for diagnosis and screening of the Alzheimer's disease.

Description

technical field [0001] The invention relates to the field of biomolecular detection, in particular to a fluorescent detection system, a fluorescent biosensor and applications thereof. Background technique [0002] Alzheimer's disease (AD) is an irreversible neurodegenerative disease mainly related to age. Alzheimer's disease affects the patient's cognition and short-term memory function. As the disease progresses, the patient's daily life ability will be gradually affected, and eventually the patient will lose the ability to take care of himself. In terms of epidemiology, the incidence of Alzheimer's disease increases exponentially with age. As the world's aging trend increases, Alzheimer's disease has become the most serious disease and cause of death after tumors and cardiovascular diseases. [0003] Due to the long incubation period of Alzheimer's disease, which can reach about 10-20 years, many patients miss the precious opportunity for early treatment because they are...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6428
Inventor 周洁许改霞
Owner SHENZHEN UNIV
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