Kit and method for detecting CEA gene mutation of peripheral blood circulating tumor cells of gastric cancer patient
A technology for peripheral blood circulation and tumor cells, applied in separation methods, chemical instruments and methods, measurement devices, etc., can solve the problems of inability to obtain tissue samples, and the inability of tissue samples to assess the CEA status of patients, avoiding edge effects and achieving good stability. , the effect of reducing the loss of cells
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[0046] Example 1
[0047] 1. Use a membrane filtration device to separate and obtain CTC in the peripheral blood of patients with advanced or recurrent gastric cancer who cannot obtain tissue samples to determine whether CTC exists:
[0048] Collect 5ml of fasting blood for 8-12 hours from the median cubital vein, and use 45ml of diluent (composition: 1mmol / L EDTA+0.1% BSA+ 0.1~10g / L soluble protein+0.1% polyglycerol fatty acid ester+PBS buffer 150mmol / L, where the percentage is volume ratio) Dilute the peripheral blood, then add 3ml of 4% paraformaldehyde to fix the diluted blood sample for 10 minutes;
[0049] In a fixed interval, assemble the membrane filtration device: as attached figure 1 , figure 2 , image 3 As shown, the filtering device is composed of a filter 3, a filter membrane 7, a blood sample container 2, a waste liquid tank 5, and an iron stand 1;
[0050] Wet the filter 3 with 10ml PBS, and then add the fixed peripheral blood sample to the blood sample container 2 o...
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