Adjustable macaque spinal cord injury method

Abstract

The invention relates to an adjustable macaque spinal cord injury method. An elastic saccule is transplanted in a target spinal cord segment of the vertebral canal of a macaque, a water injecting opening in the far end of the saccule is buried at a neighbouring subcutaneous position; normal saline of different volumes is injected in the saccule to manufacture macaque spinal cord injury models of different injury degrees. A method of using the saccule for pressing the vertebral canal of the macaque is adopted to construct the macaque spinal cord injury model of which the injury degree, the injury time interval and the injury position can be adjusted and controlled, so that the model construction effect is lasting, the injury is progressive, the injury degree behavior is visualized, trauma to the macaque is small, the lethality is low, and the method can be used for simulating and researching pathology injury mechanisms and treatment strategies of lesion of spinal cord trauma, intramedullary tumor / intradural-extramedullary tumor, intervertebral disk hernia, spinal cord congenital malformation, spinal cord infection and the like, and has important significance on preclinical test of various diseases.

Description

technical field [0001] The invention belongs to the technical field of medical animal research, and in particular relates to an adjustable spinal cord injury method for macaques. Background technique [0002] Spinal cord injury is a serious clinical disease. The life of patients with spinal cord injury is less directly threatened, and the survival period is long, but the quality of life is extremely low, and they are often unable to take care of themselves, which has caused a great burden on individuals, families and society. . Therefore, using experimental animals to prepare spinal cord injury models to study the pathological injury mechanism and treatment strategies of spinal cord injury is one of the urgent issues in biology and even the medical field. [0003] Most of the existing spinal cord injury models have been developed using rodents along with other small animals, the problem is that: [0004] 1) The spinal canal structure of rodents and other small animals is s...

AI Technical Summary

Problems solved by technology

These methods have the following problems: 1) The operation is complicated, the surgical trauma is large, and the lethality is high

2) The degree of injury cannot be adjusted. Whether it is one-sided injury or total spinal cord injury, it will directly cause direct paraplegia of the corresponding limbs. Clinically, mild symptoms such as paresthesia, fine motor disturbance, and claudication cannot be simulated.

3) The damage is irreversible, but clinically, patients with spinal cord tumors and herniated intervertebral discs will recover to a certain extent after surgery to remove the tumor or herniated intervertebral discs. The current modeling methods for spinal cord injuries in experimental animals cannot simulate the process

[0007] Therefore, based on the fact that there are few non-human primate spinal cord injury models currently available, and all of them have disadvantages, such as poor modeling effect, irreversible damage, irreversible damage, and high lethality, it is a waste of resources for macaques, a precious experimental animal.

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