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Method for extracting paeoniflorin and albiflorin from paeonia lactiflora

A technique for extracting paeonifloride glycosides and paeonifloride glycosides, which is applied in the field of extracting paeoniflorin and paeonifloride glycosides from peonies, and can solve the problems of difficult industrial production, complicated preparation process, and large solvent consumption, and achieve effective dehydration , The separation effect is obvious, and the effect of improving the wall breaking rate

Active Publication Date: 2020-09-15
YONGZHOU YIDONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the above schemes involve the purification of silica gel columns. Although the prepared products have high purity, there are overall large solvent consumption, large residues, and long processes. Silica gel columns used in industrial production have complex preparation processes, low column efficiency, and long preparation cycles. Various disadvantages such as complex detection operation, it is difficult to realize the problem of industrialized production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] An extraction method for extracting paeoniflorin and paeonifloride from Radix Paeoniae Alba, comprising the following steps:

[0034] (1) Pretreatment: select 150g of dried Radix Paeoniae Alba to be pulverized at low temperature with liquid nitrogen, and pass through a 100-mesh sieve to obtain Radix Paeoniae Alba powder;

[0035] (2) Extraction: Put the powder of Radix Paeoniae Alba in a high-voltage pulsed electric field, add solvent purified water at a rate of 70m L / min to mix with the powder of Radix Paeoniae Alba, set the temperature at 2°C, and the electric field strength at 20k V / cm. The liquid ratio is 1:8, and the initial extraction is carried out, and the extraction time is 1h; then the extract is filtered to obtain the initial extract and filter residue, and water and low-temperature cellulase are added to the filter residue for enzymolysis. The amount of low-temperature cellulase added is 0.01% of the weight of Radix Paeoniae Alba powder, the enzyme activity ...

Embodiment 2

[0043] An extraction method for extracting paeoniflorin and paeonifloride from Radix Paeoniae Alba, comprising the following steps:

[0044] (1) Pretreatment: select 300g of dried Radix Paeoniae Alba to be pulverized at low temperature with liquid nitrogen, and pass through a 100-mesh sieve to obtain Radix Paeoniae Alba powder;

[0045] (2) Extraction: Put the powder of Radix Paeoniae Alba in a high-voltage pulsed electric field, add solvent purified water at a rate of 90m L / min to mix with the powder of Radix Paeoniae Alba, set the temperature at 3°C, and the electric field strength at 20k V / cm. The liquid ratio is 1:9, and the initial extraction is carried out for 50 minutes; then the extract is filtered to obtain the initial extract and filter residue, and water and low-temperature cellulase are added to the filter residue for enzymolysis. The amount of low-temperature cellulase added is 0.02% of the weight of Radix Paeoniae Alba powder, the enzyme activity is 150000U / g, th...

Embodiment 3

[0051] An extraction method for extracting paeoniflorin and paeonifloride from Radix Paeoniae Alba, comprising the following steps:

[0052] (1) Pretreatment: select 400g of dried Radix Paeoniae Alba to be pulverized at low temperature with liquid nitrogen, and pass through an 80-mesh sieve to obtain Radix Paeoniae Alba powder;

[0053] (2) Extraction: put the Radix Paeoniae Alba powder in a high-voltage pulsed electric field, add solvent purified water at a rate of 65m L / min through a peristaltic pump and mix with the Radix Paeoniae Alba powder, set the temperature at 4°C, and the electric field strength at 25k V / cm. The liquid ratio is 1:10, and the initial extraction is carried out for 30 minutes; then the extract is filtered to obtain the initial extract and filter residue, and water and low-temperature cellulase are added to the filter residue for enzymolysis. The amount of low-temperature cellulase added is 0.02% of the weight of Radix Paeoniae Alba powder, the enzyme ac...

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PUM

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Abstract

The invention discloses a method for extracting paeoniflorin and albiflorin from paeonia lactiflora. The extraction method comprises the following steps: 1) pulverizing a paeonia lactiflora medicinalmaterial at low temperature by liquid nitrogen, and performing screening to obtain paeonia lactiflora powder; 2) performing high-voltage pulsed electric field and enzymolysis extraction on the peony powder to obtain a target extracting solution; 3) performing filtering by using an ultrafiltration membrane, and taking the ultrafiltrate; 4) decolorizing the ultrafiltrate by using a polar macroporousresin column; and (5) adsorbing the decolorized solution by using chromatographic resin, carrying out gradient elution, collecting the eluent step by step, selectively combining paeoniflorin-containing fractions and albiflorin-containing fractions in the eluent, and separately performing concentrating and drying to obtain paeoniflorin or albiflorin. According to the extraction method provided bythe invention, high-purity and high-yield paeoniflorin or albiflorin can be obtained, the method is simple, energy consumption is low, operation process conditions are easy to control, and industrialproduction is easy to realize.

Description

technical field [0001] The invention relates to the field of extraction of active ingredients of natural products, and more specifically relates to an extraction method for extracting paeoniflorin and paeoniflorin from peony. Background technique [0002] Paeonia lactiflora (scientific name: Paeonia lactiflora Pall), aliased as parting grass and prime minister of flowers, is a perennial herb belonging to the family Paeoniaceae, and its dried roots are often used as medicine. [0003] At present, there are many methods for preparing paeoniflorin or paeonifloride disclosed in the prior art, such as the patent application number CN 02133298.3 discloses a composition of paeoniflorin and paeonifloride and a preparation method thereof. The preparation process is mainly obtained by extracting with water to obtain an aqueous solution, and then eluting with ethanol water through an adsorption resin to obtain an eluent, and then concentrating and obtaining through alumina column chrom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
CPCC07H17/04C07H1/08
Inventor 张宝堂蒋乐文
Owner YONGZHOU YIDONG BIOTECH