Adenosine deaminase assay kit

Abstract

An adenosine deaminase assay kit consists of a reagent R1 and a reagent R2 which are independent from each other, wherein the reagent R1 comprises the following components: a buffer solution, a preservative, a protective agent, an anti-interference agent, a surfactant, a reaction enhancer, peroxidase, purine nucleoside phosphorylase, xanthine oxidase, 4-aminoantipyrine and water; and the reagent R2 comprises the following components: a buffer solution, a protective agent, a reaction enhancer, a preservative, adenosine, a chromogen substance and water. The adenosine deaminase assay kit is highin analysis sensitivity, good in repeatability and high in anti-interference capability, and the linearity can reach 300 U / L or above.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring adenosine deaminase. Background technique [0002] Adenosine deaminase (adenosine deaminase, ADA) is an important enzyme in nucleoside metabolism, and a nucleic acid metabolic enzyme that has an important relationship with the body's cellular immune activity. Its activity is a sensitive indicator reflecting liver damage, and can be used as one of the routine examination items of liver function. The liver enzyme language composed with ALT or GGT can comprehensively reflect the enzymatic changes of liver diseases. The determination of ADA activity in serum can be used to: judge acute liver injury and residual disease; assist in the diagnosis of chronic liver disease; assist in the diagnosis of liver fibrosis; and assist in the identification of jaundice. In addition, the lack of ADA activity is related to severe combined immunodeficiency dise...

AI Technical Summary

Problems solved by technology

[0010] At present, most commercially available kits have the following problems: insufficient analytical sensitivity, narrow linear range, poor repeatability, and weak anti-interference ability

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