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Cell labeling method and application thereof in MRI imaging of rare cells

A labeling method and cell technology, applied in chemical instruments and methods, biochemical equipment and methods, pharmaceutical formulations, etc., can solve the problems of low sensitivity, limited cell types, and difficulty in entering non-macrophages, so as to reduce patient pain, The effect of improving treatment effect, improving survival rate and quality of life

Inactive Publication Date: 2020-09-18
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems that the existing MPIO-labeled cells are limited, non-macrophages are difficult to enter, NPIO has a great impact on cell activity, and the existing MRI detection methods have low sensitivity and difficult imaging of rare cell markers difficult problem, providing a method for cell labeling

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  • Cell labeling method and application thereof in MRI imaging of rare cells
  • Cell labeling method and application thereof in MRI imaging of rare cells
  • Cell labeling method and application thereof in MRI imaging of rare cells

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Embodiment Construction

[0037] The technical solution of the present invention will be explained and described in detail below in conjunction with specific embodiments, so that those skilled in the art can better understand and implement it. Unless otherwise specified, the reagents and experimental means used in the examples are conventional products and operating methods in the art.

[0038] 1. Preparation of fluorescent magnetic microparticles:

[0039] (1) The cell penetrating peptide fragment GALFLAFLAAALSLMGLWSQPKKKRRV (SEQ ID NO.1) is fused and expressed into the SV40-VP1 (simian virus 40-vp1, simian virus 40 main capsid protein VP1) protein DE loop. The amino acid sequence of the fusion protein is as follows: As shown in SEQ ID NO.2, the gene encoding the fusion protein is shown in SEQ ID NO.3.

[0040] (2) Express the gene encoding the VP1 fusion protein in E.coli Rosetta (DE3) prokaryotic, and resuspend the cell pellet in 40mL 4°C pre-cooled packaging buffer (10mM Tris-HCl pH 7.2, 1mM Calci...

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Abstract

The present invention discloses a cell labeling method and an application thereof in MRI imaging of rare cells. The cell labeling method uses a magnetic micron particle marked by a carrier containingquantum dots to infect cells for labeling and the carrier is a virus-like particle or a targeting group; the virus-like particle is self-assembled by virus capsid proteins fused with cell-penetratingpeptide fragments; and cell-penetrating peptides are connected to the targeting group. The fluorescent magnetic micron particle used in the labeling method contains the cell penetrating peptides, canintroduce MPIO into a variety of cells in a low-toxicity and high-efficiency manner, realizes targeted positioning and precise treatment of virus host cells or tumors, realizes a treatment mode integrating multi-modal diagnosis and treatment, and thus improves a treatment effect of the tumors. Long-term in vivo tracking of the rare cells in vivo is realized, MRI imaging is conducted, the efficientand reliable method for studying a behavior mechanism of the rare cells in body is provided, and the method provides reliable technical means for in vivo imaging for studying cancer invasion and metastasis mechanisms.

Description

technical field [0001] The invention relates to the technical field of magnetic resonance imaging, in particular to a cell labeling method and its application in rare cell MRI imaging. Background technique [0002] Malignant tumors seriously endanger human health and social and economic development, and are a major public health problem in my country and the world. Tumor heterogeneity is one of the characteristics of malignant tumors. Tumor heterogeneity refers to cancers with unique genomes in the same patient. Cell subsets may accumulate in different regions of the tumor over time, which are generally divided into two types: inter-tumor heterogeneity and intra-tumor heterogeneity. Based on the study of tumor heterogeneity, scientists proposed the theory of cancer stem cells, which shows that the growth and development of many cancers are caused by a small number of tumorigenic subgroups, while the vast majority of cells The effect on cancer development is modest. If these...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/14A61K49/18C07K19/00
CPCA61K49/0002A61K49/005A61K49/0056A61K49/0067A61K49/14A61K49/1896C07K14/005C07K2319/10C12N2710/22022
Inventor 高丁张先恩门冬冯夏奕周娟
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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