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A salt-tolerant bacterial laccase, recombinant vector, recombinant bacteria, enzyme preparation and its preparation method and application

A technology of bacteria and laccase, applied in the fields of salt-tolerant bacterial laccase, recombinant carrier, enzyme preparation and its preparation, and recombinant bacteria, can solve the problems of low salt tolerance and unfavorable deep application of laccase, and achieve strong salt tolerance , the effect of maintaining enzyme activity

Active Publication Date: 2022-06-24
HENAN UNIVERSITY OF TECHNOLOGY
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AI Technical Summary

Problems solved by technology

In the actual process of dye wastewater treatment, bio-pulp, bioremediation, etc., laccase needs to be in a saline environment, but there are relatively few studies on the salt tolerance of bacterial laccase, which is not conducive to the deep application of laccase in industry

Method used

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  • A salt-tolerant bacterial laccase, recombinant vector, recombinant bacteria, enzyme preparation and its preparation method and application
  • A salt-tolerant bacterial laccase, recombinant vector, recombinant bacteria, enzyme preparation and its preparation method and application
  • A salt-tolerant bacterial laccase, recombinant vector, recombinant bacteria, enzyme preparation and its preparation method and application

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Embodiment Construction

[0027] The technical solutions of the present invention will be further described in detail below through specific embodiments.

[0028] This embodiment provides a salt-tolerant bacterial laccase whose gene is derived from Bacillus thermoglucosidase paragebacillus thermoglucosidasius (Preserved in the German Collection of Microorganisms, No. DSM 2542, available at Shanghai Baiye Biotechnology Center), use Tiangen Biochemical Technology (Beijing) Co., Ltd. P. thermoglucosidasius The laccase matures the peptide gene, analyzes its conserved sequence, and designs primers for amplification to obtain the nucleotide sequence of the salt-tolerant bacterial laccase as shown in SEQ ID NO.1, and the amino acid sequence as shown in SEQ ID NO.2.

[0029] Specifically, the specific method for extracting the genome is as follows:

[0030] 1. Take 1-5 ml of bacterial culture solution, centrifuge at 10,000 rpm (~11,500×g) for 1 min, and aspirate the supernatant as much as possible.

[0031]...

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Abstract

The invention relates to a salt-tolerant bacterial laccase, a recombinant carrier, a recombinant bacterium, an enzyme preparation, a preparation method and an application thereof. The bacterial laccase, whose gene is derived from the thermoglucosidase bacillus paragebacillus thermoglucosidasius, can be used for dye decolorization, and has high salt tolerance, and can maintain enzyme activity under severe conditions of high salinity, effectively promoting laccase in Deep application in industry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a salt-tolerant bacterial laccase, a recombinant carrier, a recombinant bacteria, an enzyme preparation, and a preparation method and application thereof. Background technique [0002] Laccase (EC1.10.3.2) is a copper-containing polyphenol oxidase widely distributed in fungi, bacteria, insects and higher plants. Laccase can use molecular oxygen as an electron acceptor to oxidize various types of phenolic and non-phenolic compounds and reduce molecular oxygen to water. Laccase is widely used in dye wastewater treatment, biological pulp, and bioremediation because it can oxidize both phenols and other highly toxic environmental pollutants. It is an emerging bioremediation agent. [0003] Compared with plant and animal laccases, microbial laccases are rich in sources, which can be divided into fungal-derived laccases and bacterial-derived laccases. Fungal laccases are genera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12N15/53C12N15/70C12N1/21C02F3/34C02F101/38C02F103/30
CPCC12N9/0061C12N15/70C02F3/342C12Y110/03002C02F2103/30C02F2101/38
Inventor 王喜凤屈建航周佳李海峰宋明珠秦志磊
Owner HENAN UNIVERSITY OF TECHNOLOGY
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