Diuretic active drug cell screening model, and establishing method and application thereof
A technology for establishing methods and active drugs, applied in the field of pharmacy, can solve the problems of high cost, long time, and difficult operation.
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Embodiment 1
[0046] Method for Establishing Cellular Screening Model of Diuretic Active Drugs
[0047] 1. Culture of MDCK cells
[0048] Purchase Madin-Daby canine kidney cells (MDCK) cell line, use DMEM high-glucose medium containing 5% fetal bovine serum, 0.5% penicillin-streptomycin solution, 94.5%, 37°C, CO 2 MDCK cells were cultured in the incubator until the logarithmic growth phase.
[0049] 2. Drug preparation
[0050] Take NaCl, DMEM high-glucose medium into 0.012, 0.015, 0.018, 0.021, 0.024, 0.027, 0.030, 0.033, 0.036g·mL -1 NaCl is set aside. Relebene, 22-hydroxyhobopentane, β-sitosterol, cycloeucalenone, zeugatene, dryocrassol, Hopan-28,22-olide, oleanolic acid, 22,28-epoxyhopane, cyclohopenol, cycloeucalenol, suberinone , (23E)-cycloart-23-ene-3β,25-diol, (23E)-cycloart-23,25-ene-3β-ol, cyclolaudenol, 11-carbonyl-β-acetylboswellic acid, 2',3' -dihydroxypropylpentadecanoate, β-carotin, and hydrochlorothiazide were dissolved in DMSO, and then diluted to 100umol L in DMEM hi...
Embodiment 2
[0059] Method for Establishing Cellular Screening Model of Diuretic Active Drugs
[0060] 1. Culture of MDCK cells
[0061] Purchase Madin-Daby canine kidney cells (MDCK) cell line, use 15% fetal calf serum, 1.5% penicillin-streptomycin solution, 83.5% DMEM high glucose medium, 37 ° C, CO 2 MDCK cells were cultured in the incubator until the logarithmic growth phase.
[0062] 2. Drug preparation
[0063]Take NaCl, DMEM high-glucose medium into 0.012, 0.015, 0.018, 0.021, 0.024, 0.027, 0.030, 0.033, 0.036g·mL -1 NaCl is set aside. Relebene, 22-hydroxyhobopentane, β-sitosterol, cycloeucalenone, zeugatene, dryocrassol, Hopan-28,22-olide, oleanolic acid, 22,28-epoxyhopane, cyclohopenol, cycloeucalenol, suberinone , (23E)-cycloart-23-ene-3β,25-diol, (23E)-cycloart-23,25-ene-3β-ol, cyclolaudenol, 11-carbonyl-β-acetylboswellic acid, 2',3' -dihydroxypropylpentadecanoate, β-carotin, and hydrochlorothiazide were dissolved in DMSO, and then diluted to 100umol L in DMEM high-glucose ...
Embodiment 3
[0072] Method for Establishing Cellular Screening Model of Diuretic Active Drugs
[0073] 1. Culture of MDCK cells
[0074] Purchase Madin-Daby canine kidney cells (MDCK) cell line, use 12.3% fetal bovine serum, 0.7% penicillin-streptomycin solution, 87% DMEM high glucose medium, 37°C, CO 2 MDCK cells were cultured in the incubator until the logarithmic growth phase.
[0075] 2. Drug preparation
[0076] Take NaCl, DMEM high-glucose medium into 0.012, 0.015, 0.018, 0.021, 0.024, 0.027, 0.030, 0.033, 0.036g·mL -1 NaCl is set aside. Relebene, 22-hydroxyhobopentane, β-sitosterol, cycloeucalenone, zeugatene, dryocrassol, Hopan-28,22-olide, oleanolic acid, 22,28-epoxyhopane, cyclohopenol, cycloeucalenol, suberinone , (23E)-cycloart-23-ene-3β,25-diol, (23E)-cycloart-23,25-ene-3β-ol, cyclolaudenol, 11-carbonyl-β-acetylboswellic acid, 2',3' -dihydroxypropylpentadecanoate, β-carotin, and hydrochlorothiazide were dissolved in DMSO, and then diluted to 100umol L in DMEM high-glucose...
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