Method for refining lovastatin

A technology of lovastatin and toluene, applied in the field of lovastatin refining, can solve the problems of non-compliance with environmental protection requirements, acetone is volatile, and the price is relatively high, and the production environment and environmental protection treatment costs are low, and odor and foreign matter are reduced. Effect

Pending Publication Date: 2020-09-29
福安药业集团烟台只楚药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the strong volatility of butyl acetate, the odor is strong and disgusting, which does not meet the requirements of environmental protection, and acetone is volatile and expensive

Method used

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  • Method for refining lovastatin
  • Method for refining lovastatin
  • Method for refining lovastatin

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Embodiment Construction

[0053] In the embodiment of the present invention, a method for refining lovastatin, the specific steps include extraction, primary refining and secondary refining;

[0054] Described extraction specifically comprises the following steps:

[0055] S1. Put 100L of fermentation broth in the fermentation bottle into the receiving tank;

[0056] S2, detect pH after stirring, monazolic acid, lovastatin content;

[0057] S3, add water, and adjust the pH to 3.00±0.50 with 15% W / V sulfuric acid;

[0058] S4, heating up to 45-65°C;

[0059] S5. Maintain 45-65°C, and detect the pH, monazolic acid, and lovastatin contents at 0, 5, 10, 15, 17, and 19 hours respectively;

[0060] S6, add 100ml toluene;

[0061] S7. Cool down to 30-45°C, stir for 3-6h, and detect the content of lovastatin;

[0062] S8, separating the toluene layer obtained, repeating the operations of S6 and S7 until the lovastatin residual amount is ≤1.5%;

[0063] S9, washing the toluene layer with 2-4% purified wat...

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Abstract

The invention discloses a method for refining lovastatin. The method comprises the steps of extraction, primary refining and secondary refining. The extraction comprises the following steps: putting afermentation flask containing fermentation broth into a receiving tank; stirring, detecting the pH value and the contents of monacolin acid and lovastatin; adding water, and adjusting the pH value to3.00 + / - 0.50 by using 15% W / V sulfuric acid; S4, heating to raise the temperature to 45 to 65 DEG C; S5, maintaining the temperature at 45 to 65 DEG C, detecting pH and the contents of monacolin acid and lovastatin at 0.5, 10, 15, 17, and 19h. Toluene extraction and methanol refining are adopted, the odor is reduced, the cost is reduced, the actual purification efficiency is high, and the foreign matters in a plant area are reduced, and the actual production environment and the environmental protection treatment cost is lower.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for refining lovastatin. Background technique [0002] Lovastatin is a secondary metabolite of Aspergillus terreus, mainly in the mycelia, and needs to be extracted with a solvent after acid hydrolysis. It turns out that we use butyl acetate to extract and refine it with acetone. Because butyl acetate is highly volatile, it has a strong odor and is disgusting, which does not meet environmental protection requirements, and acetone is volatile and expensive. Contents of the invention [0003] In order to overcome the disadvantages of the above-mentioned situation, the present invention aims to provide a technical solution capable of solving the above-mentioned problems. [0004] A method for refining lovastatin, the specific steps including extraction, primary refining and secondary refining; [0005] Described extraction specifically comprises the following steps: [0006] S1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D309/30
CPCC07D309/30
Inventor 吴新华尹国民董宏伟张卫郑召霞王莉莉
Owner 福安药业集团烟台只楚药业有限公司
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