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Heat-resistant vaccine and preparation method thereof

A vaccine and heat-resistant technology, which is applied in the field of biomedicine, can solve the problems of poor heat resistance of vaccines and complex formulations of vaccine heat-resistant protective agents, and achieve good protection effects, absolute safety, and stable properties

Pending Publication Date: 2020-10-02
广东丝源集团有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the technical problems of complex formulation of vaccine heat-resistant protective agent in the prior art, poor heat resistance of the prepared vaccine, etc., and provide a heat-resistant vaccine and its preparation method

Method used

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  • Heat-resistant vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing a heat-resistant vaccine, comprising the following steps:

[0026] S1. preparing silk globulin powder;

[0027] S2. Configure the antifreeze protection agent, wherein the solute in the antifreeze protection agent includes: the silk globulin 55g / L, lactose 100g / L, dextran 15g / L, polyvinylpyrrolidone 10g / L, glutamic acid 25g in the step S1 / L;

[0028] S3. Cool down the antifreeze protectant in step S2 to 8° C., then mix and stir the solution in step S1 and the vaccine according to the volume ratio of 1:1 for 30 minutes;

[0029] S4. Freeze-dry the mixed solution in step S3 at low temperature to obtain.

[0030] The aforementioned vaccines include live attenuated vaccines, inactivated vaccines, toxoid vaccines, vector vaccines, and edible vaccines.

[0031] The preparation method of silk globulin powder in the step S1 specifically comprises the following steps:

[0032] S11. Mix silkworm cocoons with 35-45 times the volume of deionized water, an...

Embodiment 2

[0037] A method for preparing a heat-resistant vaccine, comprising the following steps:

[0038] S1. preparing silk globulin powder;

[0039] S2. Configure the antifreeze protection agent, wherein the solute in the antifreeze protection agent includes: the silk globulin 45g / L, lactose 120g / L, dextran 40g / L, polyvinylpyrrolidone 5g / L, glutamic acid 30g in the step S1 / L;

[0040] S3. Cool down the antifreeze protectant in step S2 to 6° C., then mix and stir the solution in step S1 and the vaccine according to the volume ratio of 2:1 for 20 minutes;

[0041] S4. Freeze-dry the mixed solution in step S3 at low temperature to obtain.

[0042] The aforementioned vaccines include live attenuated vaccines, inactivated vaccines, toxoid vaccines, vector vaccines, and edible vaccines.

[0043] The preparation method of silk globulin powder in the step S1 specifically comprises the following steps:

[0044] S11. Mix silkworm cocoons with 35 times the volume of deionized water, and he...

Embodiment 3

[0049] A method for preparing a heat-resistant vaccine, comprising the following steps:

[0050] S1. preparing silk globulin powder;

[0051] S2. Configure the antifreeze protection agent, wherein the solute in the antifreeze protection agent includes: the silk globulin 50g / L, lactose 110g / L, dextran 27g / L, polyvinylpyrrolidone 7g / L, glutamic acid 27g in the step S1 / L;

[0052] S3. Cool down the antifreeze protectant in step S2 to 7° C., then mix and stir the solution in step S1 and the vaccine according to the volume ratio of 1.5:1 for 25 minutes;

[0053] S4. Freeze-dry the mixed solution in step S3 at low temperature to obtain.

[0054] The aforementioned vaccines include live attenuated vaccines, inactivated vaccines, toxoid vaccines, vector vaccines, and edible vaccines.

[0055] The preparation method of silk globulin powder in the step S1 specifically comprises the following steps:

[0056] S11. Mix silkworm cocoons with 40 times the volume of deionized water, and ...

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Abstract

The invention relates to the field of biomedicine, in particular to a preparation method of a heat-resistant vaccine. The method comprises the following steps: S1, preparing silk globulin powder; S2,preparing an antifreeze protectant; S3, cooling a mixed solution prepared in the step S2 to 6-8 DEG C, and then mixing and stirring the solution prepared in the step S1 and a vaccine for 20-30 min ata mass ratio of (1-2):1; and S4, carrying out low-temperature freeze-drying on the mixed solution prepared in the step S3. The prepared vaccine is sterile, safe and stable in character, has the characteristics of heat resistance and long storage time, and solves the problems that the vaccine needs to be frozen at low temperature and is inconvenient to store in the transportation process. The heat-resistant vaccine provided by the invention can be stored at room temperature for 6 months or more.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation method of a heat-resistant vaccine. [0002] technical background [0003] The stability of the vaccine is directly related to the size of its effective protection. At this stage, the main method to ensure the stability of vaccines is cryopreservation, that is, the use of low temperature to reduce the denaturation or inactivation speed of vaccines, thereby increasing their shelf life. There are usually two storage temperatures of 4°C and -20°C, and the corresponding cold chain system established for the transportation and storage of their products also accounts for about 80% of their total cost. However, even so, there is still no guarantee that the vaccine will not reduce its stability due to temperature fluctuations during actual use, and eventually lose its due protection. Especially in remote and underdeveloped areas where the cold chain system is lacking or imperfec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/18A61K47/26A61K47/32A61K47/36A61K9/19
CPCA61K47/42A61K47/26A61K47/36A61K47/32A61K47/183A61K9/19
Inventor 陈列辉邵辉胡智明王电梅王先燕卓新鸿张桂玲陈剑雄金鹏飞符妙婵
Owner 广东丝源集团有限公司
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