Preparation method of targeting graphene nano-grade drug carrier

A nano-drug carrier and graphene technology can be used in drug combinations, pharmaceutical formulations, medical preparations with inactive ingredients, etc., and can solve problems such as increased manufacturing costs, cumbersome and complicated processes, and poor environment, and achieves improved drug efficacy, Simple operation and good biocompatibility

Active Publication Date: 2014-01-29
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the conventional method uses these drugs to prepare targeted drug delivery systems, it is necessary to chemically graft tumor-targeting molecules such as folic acid on the outside of the designed drug carrier system. Organic solvents, resulting in increased manufacturing costs, but also have a bad impact on the environment

Method used

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  • Preparation method of targeting graphene nano-grade drug carrier
  • Preparation method of targeting graphene nano-grade drug carrier
  • Preparation method of targeting graphene nano-grade drug carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Add 0.8 mg of hydrophobic anti-tumor drug molecule doxorubicin, 1 mg of tumor-targeting molecule folic acid and 8 mg of dopamine to 40 mL of graphene nanomaterial aqueous solution with a concentration of 0.1 mg / mL; use a phacoemulsifier to disperse uniformly for 10 minutes; Adjust the pH to 8 with a pH meter; stir in the dark for 2 hours to obtain a stable complex; centrifuge at 3000 rpm for 60 minutes to collect nanoparticles, wash the particles with distilled water at least 3 times; disperse the product evenly in water to obtain Targeted nano-drug carrier based on graphene nanomaterials, spare.

Embodiment 2

[0020] Add 10 mg of hydrophobic anti-tumor drug molecule doxorubicin, 6.7 mg of tumor-targeting molecule folic acid and 100 mg of dopamine to 40 mL of graphene nanomaterial aqueous solution with a concentration of 0.5 mg / mL; use a phacoemulsifier to disperse uniformly for 40 minutes; Adjust the pH to 8.5 with a pH meter; stir the resulting stable complex on a magnetic stirrer in the dark for 5 hours; centrifuge at 7000 rpm for 40 minutes to collect nanoparticles, wash the particles with distilled water at least 3 times; homogenize the product Dispersed in a phosphate physiological buffer solution to obtain a targeted nano-drug carrier based on the graphene nano-material, which is ready for use.

Embodiment 3

[0022] Add 40 mg of hydrophobic anti-tumor drug molecule doxorubicin, 20 mg of tumor-targeting molecule folic acid and 320 mg of dopamine to 40 mL of graphene nanomaterial aqueous solution with a concentration of 1.0 mg / mL; use a phacoemulsifier to disperse uniformly for 60 minutes; Use a pH meter to adjust the pH to 10.5; stir the stable complex on a magnetic stirrer for 8 hours in the dark; use a centrifuge at 11,000 rpm for 30 minutes to collect nanoparticles, and wash the particles with distilled water for at least 3 times; homogenize the product Dispersed in a phosphate physiological buffer solution to obtain a targeted nano-drug carrier based on the graphene nano-material, which is ready for use.

[0023] The targeted nano-drug carrier system prepared by the above experiments can be seen that the process is green and environmentally friendly, which greatly reduces the use of organic reagents. The targeted graphene nano-drug carrier prepared in the above three experiments...

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Abstract

The present invention relates to a preparation method of a targeted graphene nano-drug carrier. The present invention comprises the following steps: mixing a graphene nano-material, a hydrophobic anti-cancer drug, a tumour-targeting molecule and dopamine in an aqueous solution; ultrasonically and uniformly dispersing and adjusting pH to alkaline; magnetic stirring in the dark and stabilizing for some time, then centrifuging and isolating; uniformly dispersing the product in an aqueous solution; and obtaining a targeted nano-drug carrier based on the graphene nano-material. The characteristic of the present invention is as follows: polydopamine is formed by pH-induced self-polymerization of dopamine and during the polymerization, a graphene nano-material, a hydrophobic anti-cancer drug molecule and a tumour-targeting molecule are combined at the same time to achieve the rapid preparation of a targeted drug molecule. The advantages of the present invention are as follows: a targeted nano-drug carrier containing a hydrophobic anti-cancer drug molecule can be rapidly prepared in an aqueous solution at room temperature; the technology of the method is simple and convenient to operate; and has strong practicality and a broad application prospect.

Description

technical field [0001] The invention relates to the field of novel carbon nanomaterials, in particular to a method for preparing targeted graphene nano drug carriers. Background technique [0002] Malignant tumor is a major disease that seriously threatens human health. At present, the traditional comprehensive treatment methods such as early diagnosis, radiotherapy, and chemotherapy are still used clinically. However, due to its low resolution of diseased cells and normal cells, it causes great damage to normal human tissues. Therefore, it is particularly important to develop a drug delivery system with tumor cell-specific targeting in tumor therapy. Current research has found a variety of special surface markers of tumor cells. The discovery of these tumor cell-specific surface markers provides an effective basis for designing tumor-targeted drug delivery systems. Compared with traditional radiotherapy and chemotherapy, the targeted drug delivery system has high resolu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/48A61K45/00A61P35/00
CPCA61K47/48A61K31/513A61K31/519A61K31/704A61K47/551A61K47/6949A61P35/00A61K2300/00
Inventor 林全愧黄小洁刘勇戴黎明吕帆瞿佳
Owner WENZHOU MEDICAL UNIV
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