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Stem cell culture medium and application thereof

A medium and stem cell technology, applied in the field of stem cell culture, can solve the problems of unstable stem cell culture effect, etc., and achieve the effect of improving purity and proliferation ability, enhancing lifespan and expansion ability, and eliminating free radicals.

Pending Publication Date: 2020-10-09
THE AFFILIATED HOSPITAL OF GUIZHOU MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its effect on the culture of stem cells in different tissues is unstable, and further improvement and enhancement are still needed.

Method used

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  • Stem cell culture medium and application thereof
  • Stem cell culture medium and application thereof
  • Stem cell culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A stem cell culture medium, comprising the following substances corresponding to parts by weight:

[0029] components parts by weight serum-free medium 85 copies platelet lysate 7 copies Glutamine 2 copies non-essential amino acids 3 copies multi-vitamins 2 copies Lipids 1 copy Plant Extract Additives 0.5 parts

[0030] The multivitamin is composed of the following substances in corresponding parts by weight: 10 parts of choline chloride, 3 parts of folic acid, 2 parts of inositol, 4 parts of nicotinamide, 6 parts of riboflavin, 7 parts of vitamin B12, 20 parts of vitamin C , 15 parts vitamin E.

[0031] The lipids are composed of the following substances in corresponding parts by weight: 20 parts of oleic acid, 5 parts of cholesterol, 4 parts of linoleic acid, 3 parts of lipoic acid, and 1 part of lipid.

[0032] The preparation method of described plant extract additive comprises the steps:

[0033] (1)...

Embodiment 2

[0038] A stem cell culture medium, comprising the following substances corresponding to parts by weight:

[0039] components parts by weight serum-free medium 86 copies platelet lysate 8 servings Glutamine 2.3 servings non-essential amino acids 3.5 servings multi-vitamins 2.5 servings Lipids 1.5 servings Plant Extract Additives 0.6 parts

[0040] The multivitamin is composed of the following substances in corresponding parts by weight: 10 parts of choline chloride, 5 parts of folic acid, 2 parts of inositol, 4 parts of nicotinamide, 6 parts of riboflavin, 9 parts of vitamin B12, 20 parts of vitamin C , 15 parts vitamin E.

[0041] The lipids are composed of the following substances in corresponding parts by weight: 20 parts of oleic acid, 8 parts of cholesterol, 6 parts of linoleic acid, 3 parts of lipoic acid, and 1 part of lipid.

[0042] The preparation method of described plant extract additive comprises the ...

Embodiment 3

[0048] A stem cell culture medium, comprising the following substances in corresponding parts by weight:

[0049] components parts by weight serum-free medium 87 copies platelet lysate 8.5 servings Glutamine 2.5 servings non-essential amino acids 4 parts multi-vitamins 3 copies Lipids 2 copies Plant Extract Additives 0.7 parts

[0050] The multivitamin is composed of the following substances in corresponding parts by weight: 12 parts of choline chloride, 5 parts of folic acid, 4 parts of inositol, 6 parts of nicotinamide, 9 parts of riboflavin, 9 parts of vitamin B12, 24 parts of vitamin C , 18 parts vitamin E.

[0051] The lipids are composed of the following substances in corresponding parts by weight: 24 parts of oleic acid, 8 parts of cholesterol, 6 parts of linoleic acid, 5 parts of lipoic acid and 3 parts of lipid.

[0052] The preparation method of described plant extract additive comprises the steps:

[...

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Abstract

The invention discloses a stem cell culture medium, which belongs to the technical field of stem cell culture, and comprises the following substances: a serum-free culture medium, a platelet lysate, glutamine, non-essential amino acids, composite vitamins, lipids and a plant extraction additive. The cell culture medium is suitable for culturing stem cells separated from tissues or organs with stemcells, such as bone marrow, umbilical cord, adipose tissue and the like, the amplification speed of the cells can be increased by 4-6 times, the differentiation potential of the cells is not influenced, and the purity and multiplication capacity of stem cell culture are remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of stem cell culture, and in particular relates to a stem cell culture medium and its application. Background technique [0002] Many studies have shown that stem cells can not only self-renew, but also differentiate into other functional cells under suitable conditions. Therefore, stem cells are expected to become an effective means of treating difficult human diseases. However, the content of stem cells in normal adult tissues is very small. How to rapidly expand and cultivate stem cells in vitro is an important technology for studying the mechanism of stem cells and exploring their therapeutic methods in the treatment of human diseases. [0003] The content of stem cells is as small as possible, but they are widely distributed in various tissues and organs of mammals, including but not limited to bone marrow, umbilical cord, adipose tissue, brain tissue, retina, heart, liver, lung and skin. A large numbe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N5/0667C12N5/0668C12N2500/90C12N2500/38C12N2500/32C12N2500/36C12N2500/76C12N2500/84
Inventor 刘洋李艳菊王飞清何志旭舒丽萍朱晓东刘广洋梁惠玲黄美
Owner THE AFFILIATED HOSPITAL OF GUIZHOU MEDICAL UNIV
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