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Nisin in combination with glycerol monolaurate active against helicobacterium

A technology of nisin and Helicobacter genus, which is applied in the field of nisin combined with glycerol monolaurate with anti-helicobacter activity, and can solve the problems of fungicide effectiveness barrier and so on

Inactive Publication Date: 2003-08-13
AMBI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thus, the mucus layer that lines the mucosal epithelium of the GI tract may present a barrier to the effectiveness of antimicrobial agents

Method used

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  • Nisin in combination with glycerol monolaurate active against helicobacterium
  • Nisin in combination with glycerol monolaurate active against helicobacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Synergistic bactericidal activity of nisin and GML against H. pylori in the absence of chelating agents

[0039] All bactericidal activity tests in this example were carried out at 37°C for 30 minutes. The measured parameter is the survival of bacteria exposed to GML and / or nisin as viable counts (colony forming units per ml [CFU / ml]) as determined by standard dilution and plating techniques. Media, growth and assay conditions were as follows: All Helicobacter cultures were grown in a GasPak under microaerobic conditions. H. pylori was cultured on casein tryptic soy agar + 5% defibrinated sheep blood for 2-4 days, then inoculated into NYCIII broth and grown overnight with gentle shaking. [NYCIII broth contains per liter: 1g soluble starch (Difco), 15g peptone #3 (Difco), 5g NaCl, 4g K 2 HPO 4 , 1g KH 2 PO 4 , 5 g glucose, 25 ml fresh yeast extract (Gibco), 120 ml γ-globulin-free horse serum (sigma)]. Suspensions for testing GML and nisin activity were made up with...

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Abstract

Disclosed are compositions of nisin and GML as active ingredients for the killing of Helicobacter, as well as methods for eradicating Helicobacter colonization and treatment of Helicobacter infection which employ the GML / nisin compositions.

Description

technical field [0001] The invention relates to a composition mainly composed of lanthionine-containing bacteriocin-nisin and glycerol monolaurate, and the application of the composition in treating Helicobacter bacterial infection. Background technique [0002] Much of the scientific literature dealing with the antimicrobial activity of GML or nisin discusses their activity against Gram-positive bacteria but not against Gram-negative bacteria in the absence of chelating agents or other potentiating agents. [0003] Glyceryl monolaurate (GML), also known as monolaurin, is the laurate ester of glycerol. It is used as a surfactant, preservative, and as a degreaser and make-up cleanser in cosmetics and has only limited application as a food preservative. [0004] GML is recognized as safe by the Food and Drug Administration (FDA). Title 21 of the Code of Federal Regulations (CFR) Part 184 includes monoglycerides among those compounds identified ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K31/23A61K38/00A61K38/16A61K38/48A61P1/04A61P31/04
CPCA61K31/23A61K38/482A61K38/164A61K38/48A61K45/06A61P1/04A61P31/04A61K2300/00A61K38/16
Inventor P·布莱克本B·P·戈尔茨坦D·J·库克
Owner AMBI INC
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