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A kind of microbial composite bacterial agent and its preparation method and application

A compound bacterial agent and microorganism technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as adverse environmental impact, odor generation, and reduced dissolved oxygen content, and achieve complete degradation and significant purification. The ability of water, the mild effect of the reaction process

Active Publication Date: 2018-11-27
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, many landscape pool designers lack consideration of the water purification of landscape pools in the design process, which has caused many landscape pools to have adverse effects on the environment due to the deterioration of pool water.
Especially in landscape fishponds with stagnant water, due to continuous input of feed, the eutrophication of the water is serious, and it is easy to breed algae. Affects the appearance, and algal toxins released by algae can easily kill fish
[0003] In the prior art, the repair methods for landscape pool water mainly include physical multi-stage filtration, ultraviolet disinfection, chemical disinfectant disinfection, etc. The physical multi-stage filtration method is complicated to operate, and the equipment is not easy to clean; the ultraviolet disinfection method is expensive and difficult to disinfect. The effect is poor; while chemical disinfectant disinfection will affect the fish in the landscape pond

Method used

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  • A kind of microbial composite bacterial agent and its preparation method and application
  • A kind of microbial composite bacterial agent and its preparation method and application
  • A kind of microbial composite bacterial agent and its preparation method and application

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preparation example Construction

[0028] The present invention also provides the preparation method of the above-mentioned microbial composite bacterial agent, comprising the following steps: 1) liquid fermentation culture Bacillus and faecalis streptococcus respectively to obtain Bacillus and faecalis streptococcus bacterial liquid; Streptococcus bacterial liquid is collected carrier-loaded Bacillus and diatomite carrier-loaded Streptococcus faecalis after being adsorbed by diatomaceous earth respectively; 3) the diatomite carrier-loaded Bacillus and diatomite carrier-loaded fecal Streptococci are dried at 35-40° C., pulverized, and then mixed to obtain a carrier-loaded composite microorganism; 4) The carrier-loaded composite microorganism is mixed with a trace element agent and a desiccant to obtain a microbial composite agent.

[0029] According to the invention, the bacillus and fecal streptococcus are respectively cultured by liquid fermentation to obtain the bacillus and fecal streptococcus bacteria liqui...

Embodiment 1

[0039] The microbial compound bacterial agent comprises the following raw materials in mass percentage: 50% of microorganisms, 1% of zinc sulfate, 2% of magnesium sulfate, 1% of borax, 40% of diatomite, and 6% of attapulgite; Bacillus subtilis, Bacillus licheniformis, Bacillus jellylike, Bacillus pumilus and Streptococcus faecalis in a ratio of 1:1:1:1:2.

[0040] Preparation method: Bacillus subtilis, Bacillus licheniformis, Bacillus jelly-like, Bacillus pumilus and Streptococcus faecalis were respectively activated and cultured for 24 hours, and then liquid fermentation culture was carried out for 60 hours to obtain microbial bacterial liquid. The activation medium is calculated in 1L, including 5g of beef extract, 5g of peptone, 5g of sodium chloride and the rest of water, the pH is 7.2; the activation temperature is 30°C; 1kg of peptone, 1kg of ammonium sulfate, 0.5kg of potassium dihydrogen phosphate, 0.1kg of yeast powder, 5kg of calcium carbonate and the rest of water. ...

Embodiment 2

[0043] The microbial compound bacterial agent comprises the following raw materials in mass percentage: 55% of microorganisms, 1.5% of zinc sulfate, 3% of magnesium sulfate, 1.5% of borax, 35% of diatomite, and 4% of bentonite; the microorganisms include a mass ratio of 1 : 1:1:2:2 of Bacillus subtilis, Bacillus licheniformis, Bacillus jellylike, Bacillus pumilus and Streptococcus faecalis.

[0044] Preparation method: Bacillus subtilis, Bacillus licheniformis, Bacillus jelly-like, Bacillus pumilus and Streptococcus faecalis were respectively activated and cultured for 24 hours, and then liquid fermentation culture was carried out for 72 hours to obtain microbial bacterial liquid. The activation medium is calculated in 1L, including 5g of beef extract, 5g of peptone, 5g of sodium chloride and the rest of water, the pH is 7.2; the activation temperature is 30°C; 1kg of peptone, 1kg of ammonium sulfate, 0.5kg of potassium dihydrogen phosphate, 0.1kg of yeast powder, 5kg of calci...

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Abstract

The invention provides a compound microbial agent as well as a preparation method and an application thereof and belongs to the technical field of microbial agents. The compound microbial agent is prepared from raw materials as follows: 10%-60% of microorganisms, 2%-10% of a microelement agent, 20%-70% of diatomite and 5%-10% of a drying agent, wherein the microorganisms comprise bacillus and streptococcus faecalis. With the adoption of the compound microbial agent, water of an urban landscape pool can be effectively purified; a result of water purification treatment for the landscape pool bythe compound microbial agent shows that PH and dissolved oxygen of water in the landscape pool are obviously increased, turbidity value is obviously reduced, and ammonia nitrogen, phosphate, nitrite and total chlorine of water in the landscape pool are remarkably reduced after application of the compound microbial agent.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to a microbial composite bacterial agent and its preparation method and application. Background technique [0002] The urban landscape pool can beautify the environment, decorate the courtyard, set off the artistic effect and atmosphere, and have the function of moisturizing and purifying the air, improving the urban environment. However, at present, many landscape pool designers lack consideration of the water purification of landscape pools in the design process, which has caused many landscape pools to have adverse effects on the environment due to the deterioration of pool water. Especially in landscape fishponds with stagnant water, due to continuous input of feed, the eutrophication of the water is serious, and it is easy to breed algae. It affects the appearance, and the algae toxins released by the algae are easy to kill the fish. [0003] In...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/10C12R1/07C12R1/46
CPCC02F3/34C12N1/20
Inventor 薛永秦秦薛书亚宋科乔红霞孙丽娟杨建军郑宪清李双喜吕卫光
Owner SHANGHAI ACAD OF AGRI SCI
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