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Method for researching regulation on LncRNA expression of chicken through histone acetylation

A technology for histone acetylation and expression regulation, applied in the biological field, can solve problems such as histone acetylation and LncRNA regulatory network without model methods, and achieve clear thinking, high operational feasibility, and simple and easy methods

Pending Publication Date: 2020-10-16
YANGZHOU UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, there are not many studies on the regulatory effect of histone acetylation on LncRNA genes, and there is no special model method for constructing the regulatory network of histone acetylation on LncRNA. Therefore, the present invention is essential for better understanding the function of LncRNA important

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Embodiment Construction

[0009] Required raw materials in the present invention (brackets are manufacturers): fresh fertilized eggs (Yangzhou Xianglong Poultry Industry Development Co., Ltd.), DMEM (Hyclone, SH30022.01), fetal bovine serum (Gibco, 10099141), penicillin (Solarbio , P1400-100), the DF1 cell line was frozen in our laboratory, pEGFP-N1, PGL3-Basic and pRL-SV40 and other plasmids and bacterial fluids were stored in our laboratory, competent DH5α (Tiangen), Gel Extraction Kit ( Tiangen), plasmid mini-extraction kit (Tiangen), blood / cell / tissue genomic DNA extraction kit (Tiangen), Fast Mutagenesis System Kit (Beijing Quanshijin), Max DNAPolymerase, rTaq DNAploymerase (Takara), pMD19-T (Simple) Vector Kit (Takara), T4 DNA Ligase (Takara), SnaB I, Kpn I and Xho I New Restriction Enzymes (NEB), TreliefTM SoSoo Cloning Kit (Beijing Qingke Xinye), Endo-free plasmad Mini Kit (Omega), Reporter assay System (Promega), HDTransfection Reagent (Promega), TSA (Sigma).

[0010] Concrete steps of t...

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Abstract

The invention relates to a method for researching regulation on LncRNA expression of chicken through histone acetylation and belongs to the technical field of biology. Through cloning a promoter of chicken's LncPGCR, selecting a core regulation and control zone and researching influence on activity of the promoter of the chicken's LncPGCR by histone acetylation, a foundation is laid for further researching an expression regulating and controlling mechanism of the chicken's LncPGCR. The method disclosed by the invention is easy and feasible, is clear in thinking and high in operation feasibility and can be completed in an ordinary research office. An experimenter can complete in two months. According to the method, researches on the regulation on the LncRNA expression of the chicken throughhistone acetylation are extended into the field of domestic fowls and are accurately positioned on the chicken's LncPGCR, and thus, the category of regulating the LncRNA expression in different species through histone acetylation and exerting functions is refreshed.

Description

technical field [0001] The invention relates to a method for studying the regulation of chicken LncRNA expression by histone acetylation, which belongs to the field of biotechnology. Background technique [0002] The transcription of lncRNA is strictly regulated, and its expression profile is cell-specific and tissue-specific even higher than that of protein-coding genes. Finding and discovering the causes of lncRNA expression changes in different biological processes and understanding the upstream regulatory mechanism of lncRNA are important components of the lncRNA research field, and epigenetics is an important field for studying RNA transcription regulation from the genome level. [0003] A promoter is a DNA sequence that can specifically bind to RNA polymerase and its transcription factors and initiate gene transcription, and is the most important structure for transcription regulation. Acetylation can increase the repulsion between histone and DNA by affecting histone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/65C12Q1/6897
CPCC12N15/65C12N15/85C12Q1/6897C12Q2563/107
Inventor 左其生姜景译李碧春袁霞丁颖张晨
Owner YANGZHOU UNIV