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Artificial skin and preparation method thereof

A technology of artificial skin and dermis layer, applied in the field of medical devices, can solve the problems of lack of sustained release function, poor healing effect, ignoring the bionic structure and degradation performance of the dermis layer, etc.

Pending Publication Date: 2020-10-23
湖北中部医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, although the artificial skin prepared by adding antibacterial agents directly into the dermis has its own antibacterial ability, it does not have the function of sustained release. Excessively high antibacterial concentrations in the early stage of use may bring a certain degree of damage to the wound surface and the human body. Toxic effect; the antibacterial layer is introduced between the dermis and epidermis, although the slow-release problem of antibacterial agents is solved, but the antibacterial layer is not an integral part of the wound repair function of the artificial skin. Flaws and deficiencies in growth
In addition, the existing technology mainly focuses on endowing artificial skin with antibacterial and antibacterial properties, while ignoring the bionic structure and degradation properties of the dermis, and there are problems such as poor healing effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0072] The present invention also provides a preparation method of the artificial skin of the present invention, the preparation method comprising the following steps:

[0073] Preparation of sustained-release microspheres loaded with antibacterial agents;

[0074] Prepare a pore-forming agent for the upper layer of the dermis and a pore-forming agent for the lower layer of the dermis;

[0075] Prepare a mixed solution of collagen and silk fibroin;

[0076] Mixing the mixed solution (mixed solution of collagen and silk fibroin), the pore-forming agent on the upper layer of the dermis and the slow-release microspheres loaded with antibacterial agents to obtain a matrix solution on the upper layer of the dermis;

[0077] Mixing the mixed solution (the mixed solution of collagen and silk fibroin) with the pore-forming agent in the lower layer of the dermis to obtain a matrix solution in the lower layer of the dermis;

[0078] Freeze-drying the matrix solution of the upper layer...

Embodiment 1

[0105] The preparation method of the artificial skin of the present invention specifically comprises the following steps:

[0106] (1) Preparation of silk fibroin nanospheres loaded with PHMB:

[0107] Prepare a 5 mg / mL silk fibroin solution and a 0.5 mg / mL PHMB solution, slowly drop the PHMB solution into the silk fibroin solution, mix well, freeze at -60°C for 12 hours, and thaw at room temperature to obtain PHMB-loaded Silk fibroin microsphere suspension; the suspension was centrifuged and washed twice at 5500rpm, and then passed through filter membranes with a pore size of 0.3μm and 0.1μm in turn, and finally the filtrate components with a cut-off of 0.1-0.3μm were collected, and the filtrate The components are ultrasonically dispersed and then freeze-dried to obtain PHMB-loaded silk fibroin nanospheres with a particle size of 100-300 nm;

[0108] Wherein, in the mixed solution of silk fibroin solution and PHMB solution, the mass ratio of silk fibroin to PHMB is 80:1.

...

Embodiment 2

[0125] The preparation method of the artificial skin of the present invention specifically comprises the following steps:

[0126] (1) Preparation of silk fibroin nanospheres loaded with PHMB:

[0127] Prepare 8 mg / mL silk fibroin solution and 0.8 mg / mL PHMB solution, slowly drop PHMB solution into the silk fibroin solution, mix thoroughly, freeze at -60°C for 12 hours, and thaw at room temperature to obtain PHMB-loaded Silk fibroin microsphere suspension; the suspension was centrifuged and washed twice at 5500rpm, and then passed through filter membranes with a pore size of 0.3μm and 0.1μm in turn, and finally the filtrate components with a cut-off of 0.1-0.3μm were collected, and the filtrate The components are ultrasonically dispersed and then freeze-dried to obtain PHMB-loaded silk fibroin nanospheres with a particle size of 100-300 nm;

[0128] Wherein, in the mixed solution of silk fibroin solution and PHMB solution, the mass ratio of silk fibroin protein to PHMB is 100...

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PUM

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Abstract

The invention belongs to the field of medical devices, and particularly relates to artificial skin and a preparation method thereof. The artificial skin comprises a derma layer and a cuticular layer.The artificial skin is characterized in that the derma layer comprises a derma layer upper layer and a derma layer lower layer, wherein the derma layer upper layer comprises slow release microspheresloaded with a bacteriostat, the derma layer adopts a bionic structure in bore diameter graded distribution, the bore diameter of the derma layer upper layer is smaller than that of the derma layer lower layer, the bore diameter of the derma layer upper layer is 20-100[mu]m, and the bore diameter of the derma layer lower layer is 110-200 [mu]m. According to the artificial skin provided by the invention, the slow release microspheres loaded with the bacteriostat are introduced into the derma layer upper layer, so that the artificial skin has favorable persistent bacteriostasis activity, and thebionic structure of the bore diameter gradient of the derma layer has advantages in the respects of promoting wound healing of patients and improving healing quality.

Description

technical field [0001] The invention belongs to the field of medical devices, and in particular relates to an artificial skin and a preparation method thereof. Background technique [0002] As a substitute for autologous skin grafting, artificial skin can be used for the treatment of wounds with damaged dermis, chronic wounds or burn wounds. Artificial skin has the functions of promoting wound healing and reducing scar formation. It overcomes the defect of insufficient autologous skin resources for patients with extensive burns, and gradually occupies the mainstream of the market. However, since the artificial skin fails to establish blood supply with the wound in the early stage after transplantation, its antibacterial ability is poor, and the infection rate after transplantation is high. The occurrence of infection will not only directly lead to transplant failure, but even threaten the life of the patient. [0003] At present, the commonly used clinical measures to prev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/54A61L27/56A61L27/60A61L27/58A61F2/10
CPCA61L27/60A61L27/54A61L27/56A61F2/105A61L27/26A61L27/58A61L2300/404A61L2300/622A61L2300/206A61L2300/604A61L2300/412
Inventor 叶红川杜莹莹胡伟康周雄
Owner 湖北中部医疗科技有限公司