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Solid-liquid two-phase herbal tea residue fermentation process and application thereof

A technology of liquid fermentation and solid state fermentation, applied in the field of fermentation

Pending Publication Date: 2020-10-27
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there is a lack of effective fermentation technology for herbal tea dregs to maximize the use of residual active ingredients in herbal tea dregs

Method used

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  • Solid-liquid two-phase herbal tea residue fermentation process and application thereof
  • Solid-liquid two-phase herbal tea residue fermentation process and application thereof
  • Solid-liquid two-phase herbal tea residue fermentation process and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 solid-liquid two-phase fermentation device

[0038] An example of a solid-liquid two-phase fermentation device in the present invention, specifically as figure 1 shown.

[0039] The manufacturing method of this solid-liquid two-phase fermentation device is:

[0040] Take a 50mL plastic bottle with a height of 6.8cm and a diameter of 4.2cm, start from the bottom 1cm, at intervals of 1cm in height and 1.7cm in arc length, make 8 rows of 5 holes of the same size, and the diameter of the holes is about 1mm.

[0041] After tightening the bottle cap, put it into a 200mL beaker and wrap it with newspaper or kraft paper to obtain a solid-liquid two-phase fermentation device.

Embodiment 2

[0042] Embodiment 2 A kind of solid-liquid two-phase herbal tea dregs fermentation method

[0043] A fermentation culture solution containing 1% ammonium sulfate, 0.1% dipotassium hydrogen phosphate and 0.125% potassium dihydrogen phosphate is prepared and sterilized under high pressure. Take 60g of fermentation culture liquid, add 15g of herbal tea dregs, soak for about 10min, and stir evenly.

[0044] The herbal tea dregs soaked are divided into 3 fermenting devices described in Example 1, each about 25g, and weighed accurately. Sterilize.

[0045] After sterilization, inoculate Aspergillus niger, strain concentration 2×10 9 cfu / mL, the inoculum volume was 10%. Add about 45mL of sterilized fermentation culture solution, so that two rows of holes in the plastic bottle are immersed in the nutrient solution, and then culture at 34°C and 80% humidity. At intervals of 2 days, the culture solution in the beaker was collected. After the medium in the plastic bottle was stirred...

Embodiment 3

[0052] Embodiment 3 A kind of solid-liquid two-phase herbal tea dregs fermentation method

[0053] A fermentation culture solution containing 1% ammonium sulfate, 0.1% dipotassium hydrogen phosphate and 0.125% potassium dihydrogen phosphate is prepared and sterilized under high pressure. Take 60g of fermentation culture liquid, add 15g of herbal tea dregs, soak for about 10min, and stir evenly.

[0054] The herbal tea dregs soaked are divided into 3 fermenting devices described in Example 1, each about 25g, and weighed accurately. Sterilize.

[0055] After sterilization, inoculate Aspergillus niger and Lactobacillus casei, strain concentration 2×10 9 cfu / mL, the inoculum volume was 10%. Add about 45mL of sterilized fermentation culture solution, so that two rows of holes in the plastic bottle are immersed in the nutrient solution, and then culture at 34°C and 80% humidity. At intervals of 2 days, the culture solution in the beaker was collected. After the medium in the pl...

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Abstract

The invention discloses a solid-liquid two-phase herbal tea residue fermentation process and application thereof. According to the invention, the solid-liquid two-phase fermentation is carried out onherbal tea residues, lower-layer liquid fermentation lacks oxygen, so as to promote the fermentation of probiotics, while upper-layer solid-state fermentation provides nutrients for lower-layer probiotic fermentation in a steady way, and a small amount of strains remaining in solid substrates or fermentation liquid can be used as fermentation strains for the next cycle to realize continuous fermentation without adding new strains. The herbal tea residue fermented product obtained by solid-liquid two-phase fermentation is rich in various probiotics, biological enzymes, polysaccharides, reducingsugars, organic acids, alcohol and other bioactive molecules, with extremely high economic value.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a solid-liquid two-phase herbal tea dregs fermentation process and application thereof. Background technique [0002] The existing fermentation methods are classified according to the substrate form, which can be divided into solid-state fermentation and liquid-state fermentation. However, in the existing fermentation process, fermentation always occurs in a single form, that is, only solid-state fermentation or liquid-state fermentation is used for fermentation, which cannot be achieved. The fusion of the two carries out continuous and full fermentation. [0003] In addition, most of the existing fermentation technologies use fungi for solid-state fermentation, and the fermentation products have a feedback inhibitory effect on the produced enzymes and strains, which need to be removed in time to ensure that the fermentation can continue to be effective. It takes a lot of time and la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/16C12M1/00C12N1/20C12N1/14C12R1/685C12R1/245
CPCC12M21/16C12M23/34C12M23/38C12M29/20C12N1/20C12N1/14
Inventor 徐志宏向蓉袁明贵彭新宇田雅余丹妮周廷斤
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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