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Bacillus coagulans and method for preparing L-lactic acid by using same

A technology of Bacillus coagulans and lactic acid, which is applied in the field of biological fermentation engineering, can solve the problems of decreased yield, increased energy consumption, and decreased physical and chemical indicators of products, and achieves a simple and economical fermentation process

Active Publication Date: 2021-04-23
ANHUI BBCA BIOCHEMICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At the same time, in the current large-scale L-lactic acid fermentation production process, at the end of fermentation, the residual sugar index in the fermentation broth is relatively high, about 0.5%. Higher residual sugar will lead to a decrease in yield and increase in energy consumption during extraction. Problems such as the reduction of physical and chemical indicators

Method used

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  • Bacillus coagulans and method for preparing L-lactic acid by using same
  • Bacillus coagulans and method for preparing L-lactic acid by using same
  • Bacillus coagulans and method for preparing L-lactic acid by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] This example provides a Bacillus coagulans FYLR2, which is preserved in the China Center for Type Culture Collection with the preservation number CCTCC M2020371, and its 16s rRNA sequence is shown in the sequence table as SEQ ID No: 1.

Embodiment 2

[0086] This example provides a batch fermentation method for preparing L-lactic acid in a 50L fermenter using the Bacillus coagulans provided in Example 1.

[0087] The medium composition used in this embodiment is as follows:

[0088] Incline medium: glucose 10g / L, yeast powder 10g / L, peptone 5g / L, calcium carbonate 20g / L, agar powder 20g / L, pH 6.0;

[0089] Seed medium: glucose 80g / L, yeast powder 10g / L, peptone 5g / L, calcium carbonate 40g / L, pH 6.0;

[0090] Fermentation medium: glucose 220g / L, yeast powder 0.5g / L, potassium dihydrogen phosphate 2.3g / L, dipotassium hydrogen phosphate 1.15g / L, ammonium sulfate 15g / L, sodium chloride 1g / L, dihydrogen phosphate Ammonium 0.5g / L, ferric chloride 0.3g / L, biotin 50ppm, vitamin B1 50ppm, and the balance is water; the pH of the fermentation medium is 6.0, and it is sterilized at 115°C for 20 minutes;

[0091] The preparation method is as follows:

[0092] (1) Slant culture: Bacillus coagulans FYLR2 was inoculated on the slant med...

Embodiment 3

[0097] This example provides a batch fermentation method for preparing L-lactic acid in a 50L fermenter using the Bacillus coagulans provided in Example 1.

[0098] The difference from Example 2 is that the fermentation medium of this example is: corn saccharification liquid total sugar 220g / L, yeast powder 0.5g / L, potassium dihydrogen phosphate 2.3g / L, dipotassium hydrogen phosphate 1.15g / L , ammonium sulfate 15g / L, sodium chloride 1g / L, diammonium phosphate 0.5g / L, ferric chloride 0.3g / L, biotin 50ppm, vitamin B1 50ppm, and the balance is water; The pH is 6.2, and it is sterilized at 115°C for 20 minutes.

[0099] The test results of embodiment 2 and embodiment 3 are shown in table 1:

[0100] Table 1

[0101]

[0102] From Examples 2-3 and performance tests, it can be seen that the Bacillus coagulans provided by the present invention can be used to prepare L-lactic acid, and the single-batch fermentation method has an optimal production capacity of 7.3 g / L / h, and the f...

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Abstract

The invention provides bacillus coagulans and a method for preparing L-lactic acid by using the same. The bacillus coagulans is bacillus coagulans FYLR2, the preservation number of the bacillus coagulans FYLR2 is CCTCC (China Center For Type Culture Collection) M2020371, and the nucleotide sequence of a 16s rRNA gene of the bacillus coagulans FYLR2 is shown as SEQ ID No: 1. The bacillus coagulans provided by the invention can take various substances as a fermentation carbon source, has the advantages of short single-batch fermentation time and long total fermentation time through a batch fermentation method or a continuous fermentation method, is strong in fermentation capability, can obtain fermentation liquor with extremely low carbon source residual amount or even zero carbon source residual amount, and is high in L-lactic acid concentration and extremely high in purity in the fermentation liquor.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering and relates to a production method of L-lactic acid, in particular to a Bacillus coagulans and a method for preparing L-lactic acid by using it. Background technique [0002] Lactic acid, also known as α-hydroxypropionic acid, can be divided into L-lactic acid (left-handed), D-lactic acid (dextrorotatory) and racemic lactic acid according to different optical activities. Lactic acid is a multipurpose organic acid, which can be widely used in food, medicine, chemical industry, printing and dyeing and other fields. Among them, the most important and widest industrial application is as a monomer for the synthesis of polylactic acid. Polylactic acid has good biodegradability and biocompatibility. It is not only very promising to become a substitute for petroleum-based plastics in today's increasingly scarce petroleum resources, but also considered by the industry to be the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/07
CPCC12N1/20C12P7/56
Inventor 李荣杰穆晓玲陈思弘杨金环王舒孟瑶
Owner ANHUI BBCA BIOCHEMICAL CO LTD
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