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Method for effectively improving lactic acid fermentation of biomass

A lactic acid fermentation and biomass technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of fermentable sugar loss, increase the complexity and process cost of the biorefinery process, and achieve simplified production Improvement of process, lactic acid concentration and yield, cost saving effect

Pending Publication Date: 2022-08-05
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some detoxification methods such as activated carbon adsorption, evaporation treatment, overalkaline neutralization, and enzyme or microbial detoxification have been reported (Process Biochemistry, 2004, 39:1909-1912; Biotechnology and Bioengineering, 2000, 69: 526-536) , but at the same time it also increases the complexity and process cost of the biorefining process, and also causes the loss of fermentable sugars. Therefore, if there is a protective agent that can allow microorganisms to directly ferment lactic acid in the detoxified biomass hydrolyzate , it can avoid the disadvantages of traditional detoxification process

Method used

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  • Method for effectively improving lactic acid fermentation of biomass
  • Method for effectively improving lactic acid fermentation of biomass
  • Method for effectively improving lactic acid fermentation of biomass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Influence of the addition of Tween on the fermentation of corn stover hydrolyzate to produce lactic acid

[0052] The pulverized corn stalks (20-80 mesh) and phosphorus pentoxide were mixed and ball-milled at a mass ratio of 5g / 0.4g for 30min, heat-treated at 140°C for 60min, and hydrolyzed with water at a solid-liquid ratio of 10wt% at 215°C for 45min to obtain hydrolysis. Liquid, filtered and used for later use, its main components are cellobiose 1.82g / L, glucose 21.56g / L, xylose 6.29g / L, 1.6-anhydroglucose 0.76g / L, 5-HMF 2.68g / L, furfural 4.31 g / L, acetic acid 2.70g / L. Add the following nutrients to the corn stover hydrolyzate to prepare the fermentation medium (biomass hydrolyzate fermentation medium) of the corn stover hydrolyzate: casein peptone 10.0g / L, beef extract 10.0g / L, yeast powder 10.0g / L, disodium hydrogen phosphate 4.0g / L, diammonium citrate 4.0g / L, Tween 801.0g / L, K 2 HPO 4 4.0g / L, MgSO 4 .7H 2 O 0.58g / L, MnSO 4 .H 2 O 0.25g / L, CaCO 3...

Embodiment 2

[0054] Example 2 The effect of different Tween concentrations on the fermentation of corn stover hydrolyzate to produce lactic acid

[0055] The seed solution of Pediococcus pentosaceus was 10% (V / V) inoculum (the number of cells in the fermentation broth after inoculation was 4.0*10 6 cell / mL) were respectively transferred to no Tween 80 (0 g / L) and additionally added different final concentrations ( In the experimental group of Tween-80 of 20g / L, 50g / L, 100g / L, 150g / L, 200g / L, 250g / L), the experiment group was incubated with constant temperature shaking on a shaking table at 37°C and 180rpm. The comparison of glucose consumption and lactic acid conversion in the experimental group is shown in Table 1, and the comparison of glucose and lactic acid changes in the fermentation process is shown in Table 1. figure 2 As shown, after 72h fermentation, the control group without adding Tween-80 can only consume 2.12g / L glucose to produce 1.51g / L lactic acid, while the fermentation ...

Embodiment 3

[0058] Example 3 Effects of different Tween types on the fermentation of corn stover hydrolyzate to produce lactic acid

[0059] The seed solution of Pediococcus pentosaceus was 10% (V / V) inoculum (the number of cells in the fermentation broth after inoculation was 4.0*10 6 Cell / ml) is transferred to the fermentation medium of corn straw hydrolysis liquid (which compositions in the same example 1), respectively. -40, Tween-60 or Tween-80) experimental groups were cultured with constant temperature shaking on a shaking table at 37°C and 180 rpm, and the glucose consumption and growth were detected by sampling regularly. The effect of different types of Tween on the fermentation of lactic acid by corn stover hydrolyzate such as image 3 As shown, under the action of Tween-40 and Tween-60 in the first 24h, the bacteria could consume 7.23g / L of glucose to produce 0.43g / L and 1.58g / L of lactic acid, respectively, and the fermentation stagnated after 24h. However, Tween-20 and Twe...

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Abstract

According to the method for effectively improving biomass lactic acid fermentation, biomass hydrolysate is used as a raw material, pediococcus pentosaceus is used for fermenting the biomass hydrolysate to produce lactic acid, and efficient utilization of the biomass hydrolysate can be promoted by adding high-concentration Tween 80 into hydrolysis fermentation liquor. By adding the high-concentration Tween 80 into the fermentation liquor containing the inhibitor, damage of toxic substances to cells can be reduced, the inhibition effect of the toxic inhibitor on fermentation is effectively reduced, and the lactic acid concentration and conversion rate can be increased. The pediococcus pentosaceus fermentation broth has the advantages that the pediococcus pentosaceus can directly ferment the fermentation broth containing the inhibitor under the protection of the Tween 80, and the Tween 80 can promote the pediococcus pentosaceus to degrade furfural into furfuryl alcohol, 5-HMF into 2, 5-furan-dimethyl carbinol and guaiacol into 2, 6-dimethoxyphenol in the fermentation process to carry out self-detoxification lactic acid fermentation, so that the yield of the fermented lactic acid is increased, and the yield of the fermented lactic acid is increased. Due to the characteristic, the Tween 80 can be applied to biomass lactic acid fermentation, and the Tween 80 has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and in particular relates to a method for effectively improving the lactic acid fermentation of biomass. Background technique [0002] Lactic acid is an important intermediate in the synthesis of various chemicals and can be widely used in food, medicine, cosmetics, leather, textile, environmental protection and agricultural production. The most important use today is the production of polylactic acid products from lactic acid. The production methods of lactic acid can be divided into chemical synthesis method and microbial fermentation method. The chemical method is to use acetaldehyde and hydrocyanic acid as raw materials to synthesize through a series of chemical catalytic reactions. The production cost is high, the environmental pollution is serious, and it is difficult to synthesize lactic acid with high optical purity; while the microbial fermentation method is based on sugar, starc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12N1/20C12R1/01
CPCC12P7/56C12N1/20Y02E50/10
Inventor 张宗超刘会芳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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