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Detoxification-free ethanol fermentation method of non-enzymatic cellulose hydrolyzed saccharification solution

A technology of ethanol fermentation and cellulose, applied in fermentation, biochemical equipment and methods, bulk chemical production, etc., can solve the problem of no multi-level domestication selection, continuous passage, inhibitors without stable and heritable tolerance, etc. problems, to achieve the effect of industrialized production of cellulosic ethanol, reduction of production costs, and improvement of output and efficiency

Inactive Publication Date: 2019-09-06
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method only carried out one-level domestication of Saccharomyces cerevisiae, and did not carry out multi-level domestication selection and continuous passage, and the strains did not have stable and heritable tolerance to inhibitors.

Method used

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  • Detoxification-free ethanol fermentation method of non-enzymatic cellulose hydrolyzed saccharification solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) The configuration of the acclimatization medium, specifically: adjust the pH of the non-enzymatic cellulose hydrolysis and saccharification solution to 4.5, and then mix it with the seed medium at a volume ratio of 0-20 to obtain the acclimatization medium. The specific formulation is shown in Table 1.

[0023] The non-enzymatic cellulose hydrolysis and saccharification liquid is furfural slag hydrolysis saccharification liquid: furfural slag and phosphorus pentoxide are mixed and ball milled for 30 minutes at a mass ratio of 20:1, heated to 140° C. for 60 minutes, and solid-liquid ratio is 20%. Hydrolyze at 200°C for 30 minutes to obtain a hydrolyzed saccharification slurry, and perform solid-liquid separation on the saccharified slurry to obtain a hydrolyzed saccharification liquid of furfural slag.

[0024] Its specific components are glucose 29.04g / L, xylose 7.59g / L, 1.6-anhydroglucose 1.12g / L, 5-HMF 1.66g / L, furfural 1.78g / L, cellobiose 0.50g / L, phenol 0.88 g / L...

Embodiment 2

[0035] (1) Configuration of acclimatization medium: the experimental procedure is the same as in Example 1, except that the non-enzymatic cellulose hydrolysis and saccharification solution is concentrated furfural slag hydrolysis and saccharification solution, wherein glucose is 102.94g / L and xylose is 6.07g / L , 1.6-anhydroglucose 3.50g / L, 5-HMF 3.95g / L, cellobiose 2.45g / L, phenol 2.54g / L, guaiacol 2.22g / L, vanillin 2.35g / L, syringaldehyde 2.63g / L, benzoic acid 1.84g / L.

[0036] (2) Then carry out step-by-step acclimatization culture to amylogous Saccharomyces cerevisiae to obtain domesticated strains: the experimental procedure is the same as that in Example 1, and the obtained strains are designated as strain B.

[0037] (3) Concentrate the non-enzymatic cellulose hydrolysis and saccharification solution used for the domesticated strain B to contain 140 g / L of glucose, prepare a fermentation medium, and then inoculate the strain B for ethanol fermentation.

[0038] The ferm...

Embodiment 3

[0040] (1) Configuration of domestication medium: the experimental procedure is the same as in Example 1, the difference is the change of the concentration of substances in the furfural residue hydrolyzate, which contains glucose 102.94g / L, xylose 6.07g / L, 1.6 -Anhydroglucose 3.50g / L, 5-HMF 3.95g / L, cellobiose 2.45g / L, phenol 2.54g / L, guaiacol 2.22g / L, vanillin 2.35g / L, syringaldehyde 2.63g / L, benzoic acid 1.84g / L.

[0041] (2) Then carry out step-by-step acclimatization culture to amylogous Saccharomyces cerevisiae to obtain domesticated strains: the experimental procedure is the same as that in Example 1, and the obtained strains are designated as strain B.

[0042] (3) Concentrate the non-enzymatic cellulose hydrolysis and saccharification solution used by the domesticated strain B to contain 180 g / L of glucose, then prepare a fermentation medium, and then inoculate the strain B for ethanol fermentation.

[0043] The fermentation medium is: adding MgSO to the furfural sla...

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Abstract

The invention provides a detoxification-free ethanol fermentation method of a non-enzymatic cellulose hydrolyzed saccharification solution. The method includes the following steps: performing gradualhabituated culture on starchiness saccharomyces cerevisiae by utilizing a habituated culture medium including a non-enzymatic cellulose hydrolyzed saccharification solution; and inoculating domesticated strains in the non-enzymatic cellulose hydrolyzed saccharification solution to perform detoxification-free ethanol fermentation. The lignocellulosic biomass non-enzymatic hydrolyzed saccharification solution is used for gradually domesticating the starchiness saccharomyces cerevisiae, so that the tolerance of the strains on toxic substances contained in the lignocellulosic biomass non-enzymatichydrolyzed saccharification solution can be enhanced, and the preparation of cellulose ethanol through the detoxification-free fermentation of the lignocellulosic biomass non-enzymatic hydrolyzed saccharification solution can be successfully realized; and the process is simple, and can prevent from adding more chemical reagents or other high energy consumption operation, shorten production periods and effectively reduce the production costs of the cellulose ethanol, so that the method has the potential of realizing the industrial production of the cellulose ethanol.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and in particular relates to a non-enzymatic cellulose hydrolysis and saccharification liquid non-detoxification ethanol fermentation method. Background technique [0002] Cellulose ethanol is a kind of biomass energy, which is clean, less polluting and renewable green energy. The development and utilization of biomass resources to produce cellulosic ethanol to replace petroleum-based resources will help alleviate the dependence on fossil energy, and at the same time reduce the huge damage to the environment caused by the large-scale utilization of fossil resources. Lignocellulose is a renewable biomass resource abundant in nature, which can be fermented by microorganisms to produce fuel ethanol after hydrolysis. However, natural lignocellulose has a complex structure and is difficult to be directly converted into ethanol by microorganisms. Therefore, the production process of cellulosic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12N1/36C12R1/865
CPCC12P7/10C12N1/36Y02E50/10Y02P20/54
Inventor 张宗超刘秀梅刘会芳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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