Method for efficiently producing lactic acid from non-enzymatic biomass hydrolysis saccharification liquid without detoxification

A biomass and saccharification solution technology, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems that bacteria are difficult to adapt to the fermentation system, and the composition of biomass saccharification solution is complex, etc., to achieve Good inhibitor tolerance, shortened production cycle, and reduced production cost

Pending Publication Date: 2022-03-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above studies have obtained strains with better tolerance, the composition of the biomass saccharification solution is very complex, and the toxic substances are not limited to the above-mentioned clear ones, so it is difficult for the disclosed strains to adapt to more complex fermentations system

Method used

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  • Method for efficiently producing lactic acid from non-enzymatic biomass hydrolysis saccharification liquid without detoxification
  • Method for efficiently producing lactic acid from non-enzymatic biomass hydrolysis saccharification liquid without detoxification
  • Method for efficiently producing lactic acid from non-enzymatic biomass hydrolysis saccharification liquid without detoxification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Rice husk dregs hydrolyzate domestication experiment of Pediococcus pentosaceae

[0050] (1) Preparation of non-enzymatic rice husk hydrolysis saccharification liquid: rice husk (obtaining method: 150g rice husk and 150g deionized water (containing 5wt% H 3 PO 4 ) into a pressure-sealed stainless steel hydrolysis reactor, keep it under saturated steam at 205-210°C for 30 minutes, then open the valve quickly, filter the collected wet rice husk under pressure, and then dry it at room temperature under ventilated conditions until the water quality The content is less than 10%. ) mixed with phosphorus pentoxide at a mass ratio of 20:1 and ball-milled for 30 minutes, heat-treated at 140°C for 60 minutes, hydrolyzed at 215°C for 45 minutes at a solid-to-liquid ratio of 10wt% (g / ml) to obtain a hydrolyzed saccharification slurry, and the saccharification slurry was The rice husk slag hydrolysis and saccharification liquid is obtained by solid-liquid separation, and...

Embodiment 2

[0056] Example 2 Fermentation of wild bacteria and domesticated bacteria A to produce lactic acid from hydrolysis and saccharification liquid of rice husk dregs-batch fermentation comparative experiment

[0057] Single colonies of wild bacteria and domesticated bacteria A were inoculated into seed medium for overnight culture to obtain seed liquid, and the seed liquid was transferred to rice husk residue hydrolysis and saccharification liquid at 37 ℃, 180rpm shaker constant temperature shaking culture fermentation, the comparison of the growth trend is as follows Figure 4 As shown, the wild bacteria grow slowly, the cell density is small, and the highest OD value is only 12.90, while the domesticated bacteria A has a large cell density, and the highest OD value can reach 18.06. The changes of substrate products in the fermentation broth are as follows: Figure 5 As shown, the wild bacteria can slowly consume glucose in the first 42 hours, the highest concentration of lactic ...

Embodiment 3

[0058] Example 3 High-concentration glucose re-acclimated Pediococcus pentosaceae fermented rice husk residue hydrolyzate experiment

[0059] Because domesticated bacterium A is slower to the utilization rate of glucose in the hydrolyzate, therefore utilize similar domesticated method to increase its glucose concentration (50g / L, 80g / L, 108g / L, 137g / L, 162g / L, 204g / L L) is domesticated and cultivated again in the re-acclimated fermentation medium to accelerate its utilization of glucose and strengthen its adaptability to acetic acid and lactic acid. First, the seed solution of domesticated bacteria A was transferred to the re-acclimation medium with an initial glucose concentration of 50g / L at an inoculum of 10% for cultivation, and then transferred to glucose with an inoculum of 10% after the glucose was exhausted. Concentration is 80g / L acclimatization culture in the acclimatization medium, so method until initial glucose concentration is the acclimatization medium of 204g / L...

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Abstract

The invention provides a detoxification-free high-efficiency lactic acid production method using a non-enzymatic biomass hydrolysis saccharification liquid as a raw material, which comprises the following steps: firstly, carrying out ball milling-hydrolysis-solid-liquid separation on phosphorus pentoxide and biomass to obtain the non-enzymatic biomass hydrolysis saccharification liquid; pediococcus pentosaceus is subjected to step-by-step domestication culture by using saccharified liquids with different concentrations to obtain preliminary domestication bacteria, then the preliminary domestication bacteria are domesticated by using glucose gradients with different concentrations to obtain dominant domestication bacteria, and the obtained dominant domestication bacteria are used for fermenting non-enzymatic biomass hydrolysis saccharified liquids to produce lactic acid. The domesticated bacterium has good inhibitor tolerance, glucose and xylose can be utilized at the same time, and the yield of lactic acid is high. According to the technology, raw materials are cheap, the process is simple, addition of cellulase, more chemical reagents or other high-energy-consumption operation is avoided, the production period is shortened, the production cost of lactic acid is effectively reduced, and the potential of industrial production of lactic acid is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and in particular relates to a method for efficiently producing lactic acid without detoxification from non-enzymatic biomass hydrolysis and saccharification liquid. Background technique [0002] Lactic acid is an important intermediate in the synthesis of various chemicals and can be widely used in food, medicine, cosmetics, leather, textiles, environmental protection and agricultural production. The most important use at present is to produce degradable polylactic acid products (Polymeric lactic acid, PLA) from lactic acid that can replace polymer materials such as polyethylene and polypropylene. Polylactic acid has the advantages of biodegradability, biocompatibility, strong elasticity and good drug release controllability. Based on these advantages, it is widely used in surgical sutures, protective clothing, slow-release drugs, and covering films. , garbage bags, rigid containers and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12N1/36C12N1/20C13K1/02C12R1/01
CPCC12P7/56C12N1/36C12N1/20C13K1/02C12P2201/00C12P2203/00
Inventor 张宗超刘会芳刘秀梅姜宏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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