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Livestock manure decomposed compost complex microbial inoculant and preparation method and application thereof

A technology of compound bacterial agent and livestock manure, which is applied in the field of microorganism and livestock manure decomposed composting compound bacterial agent, can solve the problems of long composting period, short high temperature period, and prolonging composting period, and achieves strong cellulose decomposition ability, prolonging The effect of high temperature stage and sufficient duration

Inactive Publication Date: 2020-10-30
榆林沙漠王生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) Use more medium-temperature microorganisms. Although it can shorten the composting start-up fermentation time, the subsequent high-temperature period will basically kill the fermentation microorganisms, resulting in too short a high-temperature period, incomplete decomposition, and a long composting cycle;
[0007] (2) Neglecting the selection of high-efficiency microorganisms for refractory substances such as cellulose, and can only rely on the long-term anaerobic conditions of microorganisms in the compost environment to decompose at high temperature, prolonging the composting cycle

Method used

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  • Livestock manure decomposed compost complex microbial inoculant and preparation method and application thereof
  • Livestock manure decomposed compost complex microbial inoculant and preparation method and application thereof
  • Livestock manure decomposed compost complex microbial inoculant and preparation method and application thereof

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preparation example Construction

[0039] The preparation method of a kind of poultry manure decomposing and composting composite bacterial agent of the embodiment of the present invention comprises the following steps:

[0040] (1) Strain activation: Bacillus acidophilus, Bacillus thuringiensis, Bacillus high temperature X1, Bacillus high temperature X2, Bacillus high temperature X3, Klebsiella, Klebsiella subsp. The original strains of 11 species of Aspergillus, Phalaenopsis, Bacillus licheniformis and Fusarium equiseti were respectively inoculated on the corresponding solid medium (LB solid medium, PDA solid medium), and then the Eight strains of Acidobacillus, Bacillus thuringiensis, Bacillus thermostable X1, Bacillus thermostable X2, Bacillus thermostable X3, Klebsiella, Klebsiella subsp. Cultivate at 25°C for 1 to 2 days, and culture the three kinds of bacteria, Aspergillus niger, Phylaea genus and Fusarium equiseti, at 25°C for 3 to 4 days to activate the strains;

[0041] (2) Expansion culture: Bacillus ...

Embodiment 1

[0049] The preparation of a kind of composite bacterial agent of the embodiment of the present invention comprises the following steps:

[0050] (1) Strain activation: Bacillus acidophilus, Bacillus thuringiensis, Bacillus high temperature X1, Bacillus high temperature X2, Bacillus high temperature X3, Klebsiella, Klebsiella subsp. The original strains of 11 species of Aspergillus, Phalaenopsis, Bacillus licheniformis and Fusarium equiseti were respectively inoculated on the corresponding solid medium (LB solid medium, PDA solid medium), and then the Eight strains of Acidobacillus, Bacillus thuringiensis, Bacillus thermostable X1, Bacillus thermostable X2, Bacillus thermostable X3, Klebsiella, Klebsiella subsp. Cultivate at 25°C for 1 to 2 days, and then culture the three types of bacteria, Aspergillus niger, Phalaenopsis genus and Fusarium equiseti, at 25° C. for 3 to 4 days to activate the strains.

[0051] (2) Expansion culture: Bacillus acidophilus, Bacillus thuringiensis...

Embodiment 2

[0055] The application of a kind of composite bacterial agent of the embodiment of the present invention in poultry manure composting comprises the following steps:

[0056] (1) Mix fresh poultry manure (such as cow manure, pig manure) with crushed crop straw particles (such as 2-3cm long corn stalks) in a certain proportion, so that the carbon-nitrogen ratio of the mixture is about 26-30 , the moisture content is 60% to 65%. The solid composite bacterial agent is prepared according to the quality of the pile material 6‰~10‰, and a layer of solid composite bacterial agent is spread on a layer of pile material, and the pile material and the bacterial agent are evenly mixed to form a 3.5-meter-long, 1.8-meter-wide m and 1 m high strip-stack composting fermenters, and a control group was established for natural composting. Measure the internal temperature of the pile. If it is greater than 50°C, start to turn the pile every three days. When the temperature drops to 35°C, turn it...

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Abstract

The invention discloses a livestock manure decomposed compost complex microbial inoculant and a preparation method and application thereof. The complex microbial inoculant is composed of a starch decomposition strain, a cellulose decomposition strain and a protein decomposition strain, wherein the starch decomposition strain is one or more of acidophilic bacillus, bacillus thuringiensis, high-temperature-resistant bacillus X1 and high-temperature-resistant bacillus X2, the cellulose decomposition strain is one or more of high-temperature-resistant bacillus X3, klebsiella and klebsiella subspecies, and the protein decomposition strain is one or more of aspergillus niger, Botrytis fungi, bacillus licheniformis and fusarium moniliforme. The complex microbial inoculant provided by the invention can be heated to a high temperature period in a short time, the duration of the high temperature period is sufficient, and the complex microbial inoculant has the characteristics of strong cellulosedecomposition capability and shortened composting period.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, relates to the field of decomposed and composted poultry manure compound bacterial agent, in particular to a poultry and livestock manure decomposed and composted compound bacterial agent and its preparation method and application. Background technique [0002] The continuous improvement of scale, intensification and industrialization of the breeding industry has made the demand for the disposal of poultry manure produced by the breeding industry increasingly urgent. Due to the neglect of environmental protection in the early stage, poultry manure was discharged randomly without treatment, causing serious environmental pollution, making poultry manure pollution one of the three major sources of water environment pollution alongside industrial wastewater and domestic sewage; even worse Make aquaculture pollution and industrial pollution tied for my country's two major sources of pollution. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/08C05F17/20C05F17/50C05F15/00C12R1/07C12R1/22C12R1/10C12R1/685C12R1/77C12R1/645
CPCC05F3/00C05F11/08C12N1/14C12N1/20C05F17/20C05F17/50C05F11/00Y02W30/40
Inventor 李宇刘永军张至张驰白慧
Owner 榆林沙漠王生物科技有限公司
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