Salmonella nucleic acid detection plasmid DNA reference sample, preparation method and application thereof

Abstract

The invention discloses a salmonella nucleic acid detection plasmid DNA reference sample and a preparation method and application thereof and belongs to the field of microbiological detection. The reference sample is formed by inserting fragments containing salmonella typhimurium specific sequence se, salmonella typhimurium specific sequence st and common aceA genes into plasmids, the genes are connected in series according to a ratio of 1: 1: 1, the genes are separated by irrelevant gene fragments, the reference product contains three clear detection gene segments, i.e. Se, st and aceA, has clear sequence sources, is stored in plasmids in a single copy mode, can be used for qualitative detection, and can also be used as a reference product with traceable quantity values to carry out reagent performance identification and capability evaluation between laboratories.

Description

technical field [0001] The invention relates to the field of microorganism detection, in particular to a salmonella nucleic acid detection plasmid DNA reference sample, a preparation method and an application thereof. Background technique [0002] Salmonella (Sa) belongs to the Enterobacteriaceae family and is a foodborne pathogenic microorganism that threatens the health of humans and animals. It can cause severe diseases such as typhoid, paratyphoid, gastroenteritis, and sepsis. Among the cases of bacterial food poisoning in various countries in the world, the food poisoning caused by it often ranks first or second. Salmonella is very common in everyday life, such as in raw egg shells, poultry, red meat, among other things, contaminated water is a major source of salmonella worldwide. It has been confirmed that about 20 million people in developing countries are infected with Salmonella. Similarly, the United States, Germany and other countries are also frequent places fo...

AI Technical Summary

Problems solved by technology

However, there is a lack of positive nucleic acid reference materials for PCR at present.

The simultaneous identification and type analysis of Salmonella often requires the use of multiple genomic reference products, and the value of the target gene in the genomic reference product is difficult to determine and trace. Therefore, the use of genomic DNA as a reference product has the following disadvantages: Difficulty providing traceable quantitative references for multiple targets simultaneously

Therefore, there are serious deficiencies in the detection effect and evaluation of laboratory results.

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