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Method for detecting hair water in fermented soy sauce product

A technology of hair water and soy sauce, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc. It can solve the problems of high level requirements for experimenters, unfavorable rapid detection, and complicated pretreatment. and other problems, to achieve the effect of low experimental environment requirements, stable phenomena and high sensitivity

Active Publication Date: 2020-11-03
广州智汇生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pretreatment of this method is complicated, requiring the use of a large and expensive instrument such as gas chromatography-mass spectrometer, which is difficult to operate, high in cost, and requires high levels of experimenters. It is limited to routine testing in laboratories, which is not conducive to rapid detection and results. goals and requirements

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  • Method for detecting hair water in fermented soy sauce product
  • Method for detecting hair water in fermented soy sauce product
  • Method for detecting hair water in fermented soy sauce product

Examples

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Embodiment 1

[0047] The sodium hydroxide in this embodiment is selected from the analytically pure sodium hydroxide supplied by Sinopharm Group Chemical Reagent Co., Ltd., the sodium acetate is selected from the analytically pure anhydrous sodium acetate supplied by Sinopharm Group Chemical Reagent Co., Ltd., and the glacial acetic acid is selected from Sinopharm Group Chemical Reagent Co., Ltd. For analytical pure glacial acetic acid and sodium chloride, choose analytical pure sodium chloride supplied by Sinopharm Chemical Reagent Co., Ltd. For activated carbon, choose analytical pure activated carbon powder supplied by Sinopharm Chemical Reagent Co., Ltd. For anhydrous ether, choose Sinopharm Chemical Reagent Co., Ltd. The analytical pure anhydrous diethyl ether supplied, the tetrahydrofuran solution of methylmagnesium chloride selects the 3.0mol / L tetrahydrofuran solution of methylmagnesium chloride supplied by Sinopharm Chemical Reagent Co., Ltd., and the cuprous chloride selects the ana...

Embodiment 2

[0055] A method for detecting hair water in fermented soy sauce products of the present invention comprises the following:

[0056] Step a, pre-treatment: Measure 1.0ml of soy sauce sample in a centrifuge tube, add 2g of sodium chloride, 2.0g of activated carbon, 0.2g of iron filings and 5ml of water, mix well, add 5ml of anhydrous ether, and mix at 3500 rpm Centrifuge at a speed of 1 / min for 5 minutes, take 4.5ml of the upper layer solution and divide it into three equal parts, namely the first sample solution, the second sample solution and the third sample solution;

[0057] Adding sodium chloride in this step can increase the precipitation rate of the indicator chloropropanol, which facilitates the subsequent extraction of chloropropanol. Iron filings and activated carbon were added to remove the color interference of the soy sauce samples. Anhydrous diethyl ether was added to extract chloropropanol and provide anhydrous environment for subsequent reactions.

[0058] Ste...

Embodiment 3

[0079] A method for detecting hair water in fermented soy sauce products of the present invention comprises the following:

[0080] Step a, pre-treatment: Measure 3.0ml of soy sauce sample in a centrifuge tube, add 3.0g of sodium chloride, 2.5g of activated carbon, 0.5g of iron filings and 10ml of water, add 5ml of anhydrous ether after mixing, and mix at 4000 Centrifuge at a speed of rpm for 6 minutes, take 4.5ml of the upper layer solution and divide it into three equal parts, namely the first sample solution, the second sample solution and the third sample solution;

[0081]Step b, determination of the first sample liquid: add 0.10ml of 3mol / L tetrahydrofuran solution of methylmagnesium chloride to the first sample liquid, shake and mix, add 0.4g of the first catalyst, the first catalyst is cuprous bromide, Add anhydrous diethyl ether to make the volume of the solution 20ml, stir at room temperature at 500 rpm for 20 minutes, and then filter to obtain the first solution to ...

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Abstract

The invention discloses a method for detecting hair water in a fermented soy sauce product. The method comprises the following steps: step a, carrying out pretreatment; b, measuring a first sample solution; c, measuring a second sample solution; d, determining a third sample solution; step e, carrying out qualitative judgment. According to the detection method, chloropropanol is used as an indicator of the hair water, and is converted into acetylacetone through reaction to be used as a detection target object, so that whether the soy sauce sample contains the hair water or not is judged. The detection method is simple and convenient to operate, stable in phenomenon, rapid, obvious, high in specificity and high in sensitivity, does not need a large analytical instrument, has low requirements on an experimental environment, greatly reduces the occurrence of false positive conditions, and is suitable for rapidly detecting whether the fermented soy sauce product contains the hair water ona large scale.

Description

technical field [0001] The invention relates to the field of soy sauce detection, in particular to a method for detecting hair water in fermented soy sauce products. Background technique [0002] In recent years, some criminals have added hair hydrolyzate to soy sauce and other products to replace grain fermentation such as soybeans, increase the content of false amino acids in products, and artificially synthesize fake soy sauce. These hair-processed soy sauce and other products are cheap to produce and are price-competitive in the market to deceive consumers. Hair water is the use of human or animal hair. Because it is rich in protein, it is hydrolyzed by acid to produce amino acid liquid, which is illegally added to food as a nutritional ingredient. In 2009, the "List of Non-edible Substances That May Be Illegally Added in Food (Third Batch)" issued by the Ministry of Health clearly listed hair water as non-edible substances, and the key product is soy sauce seasoning. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/82
CPCG01N21/78G01N21/82
Inventor 李丰毓志超文永贤
Owner 广州智汇生物科技有限公司