Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of anti-shedding glass slide

A glass slide and detachment prevention technology, which is applied in the preparation, sampling, and instrumentation of test samples, can solve the problems of sample detachment and other problems, and achieve the effect of improving adsorption efficiency, reducing sample detachment rate, and firm adsorption

Active Publication Date: 2022-01-18
ZHUHAI LITUO DEV +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a preparation method of anti-shedding glass slides, to solve the problem of sample stripping in the process of histoimmunology experiments, and to improve the adsorption efficiency and adsorption stability of cells in the process of cell immunology experiments

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1: Processing slides

[0025] Heat the purified water to 70°C, add the laundry powder into the hot water according to the mass ratio of washing powder (Liby non-phosphorus washing powder): purified water: 1:500, stir until completely dissolved, dissolve and let stand. Allow slides to be completely submerged in detergent water. Soak for 30 minutes, and keep the water temperature above 50°C throughout the soaking process. After soaking, rinse immediately under running water until there is no foam and no marks left. Dehydrate the cleaned slides in 95% ethanol for 1-2 minutes and take them out. After the alcohol on the glass slide evaporates naturally, put it in an oven and store it for later use.

[0026] 2: glue

[0027] A: The anti-degumming solution is prepared by dissolving 0.2g chitin in 3ml water. Then add 180ml absolute ethanol.

[0028] B: The glass slide is facing upwards and placed horizontally on the plexiglass plate. Check the front and back of each plexi...

Embodiment 2

[0032] 1: Processing slides

[0033] Heat the purified water to 70°C, add the laundry powder into the hot water according to the mass ratio of washing powder (Liby non-phosphorus washing powder): purified water: 1:500, stir until completely dissolved, dissolve and let stand. Allow slides to be completely submerged in detergent water. Soak for 30 minutes, and keep the water temperature above 50°C throughout the soaking process. After soaking, rinse immediately under running water until there is no foam and no marks left. Dehydrate the cleaned slides in 95% ethanol for 1-2 minutes and take them out. After the alcohol on the glass slide evaporates naturally, put it in an oven and store it for later use.

[0034] 2: glue

[0035] A: The anti-degumming solution is prepared by dissolving 0.1g chitin in 3ml water. Then add 180ml absolute ethanol.

[0036] B: The glass slide is facing upwards and placed horizontally on the plexiglass plate. Check the front and back of each plexi...

Embodiment 3

[0040] 1: Processing slides

[0041] Heat the purified water to 70°C, add the laundry powder into the hot water according to the mass ratio of washing powder (Liby non-phosphorus washing powder): purified water: 1:500, stir until completely dissolved, dissolve and let stand. Allow slides to be completely submerged in detergent water. Soak for 30 minutes, and keep the water temperature above 50°C throughout the soaking process. After soaking, rinse immediately under running water until there is no foam and no marks left. Dehydrate the cleaned slides in 95% ethanol for 1-2 minutes and take them out. After the alcohol on the glass slide evaporates naturally, put it in an oven and store it for later use.

[0042] 2: glue

[0043] A: Anti-degumming liquid preparation, dissolve 0.3g chitin in 3ml water, then add 180ml absolute ethanol.

[0044] B: The glass slide is facing up and placed horizontally on the plexiglass plate. Every time a plexiglass plate is filled, check the fro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of anti-shedding glass slides, which belongs to the technical field of glass slides; it includes the following steps, and the following steps are carried out in sequence; step S1, treating the glass slides, according to the mass ratio of washing powder: purified water is 1:500 Add washing powder into hot water, stir until fully dissolved, and configure cleaning solution; then soak the glass slide in washing powder water; after cleaning, put it in an oven and save it for later use; step S2, anti-degumming liquid configuration, put Weigh a certain amount of chitin and dissolve it in the corresponding volume of water; then add the corresponding volume of absolute ethanol, stir well to make it fully dissolve without floc; The liquid device absorbs the anti-degumming liquid, and points it in the circular area of ​​the glass slide, so that the anti-degumming liquid spreads naturally on the glass slide; step S3, drying, put the glass slide coated with the glue solution into a constant temperature drying box dry. The invention can improve the adsorption efficiency of the sample and the stability of the sample after adsorption.

Description

technical field [0001] The invention belongs to the technical field of glass slides, and in particular relates to a preparation method of an anti-shedding glass slide. Background technique [0002] In recent years, immunohistochemistry has been widely used in in vitro detection. When making plant slices and tissue slices, paraffin sections need to use adhesives. fall off. Protein paste, gelatin paste, and collodion paste can be used for conventional section staining, but in some special histochemical staining, such as immunohistochemistry, it often cannot withstand repeated shaking washing, trypsin digestion, etc., resulting in desquamation. film, impact observation and result analysis. In order to ensure the normal progress of the immunohistochemical experiment, it is required to properly treat the slides before the patch, and the adhesive must be treated on the cleaned slides to prevent the slides from falling off. Therefore in order to solve this problem, we have desig...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/44
CPCG01N1/2813G01N1/28G01N1/30G01N1/44
Inventor 雍学安
Owner ZHUHAI LITUO DEV