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Method for sampling peanut seeds in different development stages

A technique for peanuts and kernels, which is applied in the field of crop physiology and genetics, can solve the problems of large sampling errors and great differences in the state of pod expansion and development at the time of burying the soil, achieve short marking time, improve sampling accuracy and consistency, and maintain The effect of synchronicity

Pending Publication Date: 2020-11-24
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the long flowering period of peanuts, it takes 30-50 days from the beginning of flowering to the end of the flowering period, and even if the flowers at different nodes bloom on the same day, the elongation of the fruit needles, the time of entering the soil, and the state of pod swelling and development are also very different, resulting in greater sampling error

Method used

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  • Method for sampling peanut seeds in different development stages
  • Method for sampling peanut seeds in different development stages
  • Method for sampling peanut seeds in different development stages

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The sampling method of embodiment 1 peanut kernel different development stages

[0034] A method for sampling peanut kernels at different development stages, comprising the following steps:

[0035] (1) Sowing: sow the peanut varieties Yuhua 65, Yuhua 37, and Kainong 1715 with high oleic acid content, and the peanut varieties Yuhua 9326 and Yuhua 22 with ordinary oleic acid content in the field respectively, and manage them according to field routines;

[0036] (2) Marking fruit needles: During the flowering and needle peak stage of peanuts, when there are 4 to 6 fruit needles that have not yet entered the soil at the second or third node of each peanut, select the fruit needles with a length greater than 2cm, and use fine The thread is bundled and marked, and the marking is completed within 1 to 3 days.

[0037] (3) Concentrated soil cultivation: After marking the fruit needles, carry out uniform soil cultivation at the same time, so that the marked fruit needles are ...

Embodiment 2

[0039] Example 2 Fatty Acid Accumulation Laws of Peanut Kernels at Different Developmental Stages

[0040] The peanut kernel samples of different varieties and different development stages of peanut kernels sampled and preserved in Example 1 were sent to the Agricultural Products Testing Center of the Ministry of Agriculture (Zhengzhou) to measure the contents of oleic acid, linoleic acid, palmitic acid and behenic acid respectively. The samples were repeated 3 times, and the results showed that the coefficient of variation among the 3 repetitions of different varieties and different development stages was small, indicating that the sampling error was effectively controlled (as shown in Table 1). The dynamic accumulation curves of the content analysis of oleic acid, linoleic acid, palmitic acid and behenic acid in different peanut kernels at different developmental stages are as follows: Figure 4-7 shown.

[0041]

[0042] Depend on Figure 4 It can be seen that the accu...

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Abstract

The invention relates to a method for sampling peanut seeds in different development stages, which comprises the following steps of: sowing a peanut variety to be detected in a field, and performing conventional field management; in the full flowering and needle setting period of the peanuts, when 4-6 fruit needles which are not buried in soil appear at the second node or the third node of each peanut, selecting the fruit needles with the length larger than 2 cm for marking; after fruit needle marking is finished, carrying out unified hilling at the same time, so that the marked fruit needlesare completely covered; marking the day of unified hilling as the first day when the fruit needles are buried in the soil, and then taking the peanut seed kernel samples once every 5-10 days for lateruse. According to the method, the marking time is short, the synchronism of pod development is maintained to the maximum extent, the sampling error caused by pod development difference is controlled,the sampling accuracy and consistency are improved, and a foundation is laid for further physiology, biochemistry or genetics and molecular biology research.

Description

technical field [0001] The invention relates to a sampling method for different development stages of peanut kernels, belonging to the field of crop physiology and heredity. Background technique [0002] Peanut (Arachis hypogeae L.) is a crop that blooms above ground and bears fruit underground. Its pods and seeds are the main product organs of peanuts, rich in fat and protein, and are the main source of vegetable oil and protein for human consumption. Based on different market demands and processing purposes, the main goal of peanut breeding is to select peanut varieties with high yield of pods or kernels, high fat or protein content in kernels, and different fatty acid compositions. In-depth understanding of the growth and development of peanut pods and kernels, as well as the accumulation of fat, protein and fatty acids, is of great significance for the breeding of new peanut varieties. However, the underground fruiting characteristics of peanuts make it very difficult t...

Claims

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Application Information

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IPC IPC(8): G01N1/04
CPCG01N1/04
Inventor 张新友吕登宇黄冰艳张毛宁房元瑾董文召石磊苗利娟王娟齐飞艳
Owner HENAN ACAD OF AGRI SCI