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Micro tissue paraffin embedding pretreatment kit and use method thereof

A technology of paraffin embedding and kits, which is applied in the field of kits, can solve the problems of large experimental errors and poor results, and achieve the effects of fewer experimental steps, lower experimental costs, and reliable data

Pending Publication Date: 2020-11-24
THE AFFILIATED HOSPITAL OF TRADITIONAL CHINESE MEDICAL TO SOUTHWEST MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the shortcomings of the existing micro-tissue fixation, dehydration, transparency and other experimental steps in the prior art, which have poor effects and large experimental errors, and propose a micro-tissue pretreatment kit for paraffin embedding and How to use it

Method used

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  • Micro tissue paraffin embedding pretreatment kit and use method thereof
  • Micro tissue paraffin embedding pretreatment kit and use method thereof
  • Micro tissue paraffin embedding pretreatment kit and use method thereof

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Embodiment Construction

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0024] refer to Figure 1-4 , a micro tissue paraffin embedding pretreatment kit, comprising a kit body 1, a cover plate 3 and an operating orifice plate 4, the operating orifice plate 4 is provided with a reaction hole, a filter groove 48, a tweezers groove 49, and an absorbent paper use groove 401 and absorbent paper storage tank 402, the reaction holes are arranged counterclockwise and include 40% polymethanol hole 41, 70% alcohol hole 42, 85% alcohol hole 43, 95% alcohol hole 44, 100% alcohol hole 45, two Toluene holes 46 and liquid paraffin holes 47, wherein the number of 95% alcohol holes 44, 100% alcohol holes 45 and xylene holes 46 are two, and the number of ...

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Abstract

The invention relates to the technical field of kits. The invention in particular relates to a micro tissue paraffin embedding pretreatment kit. The kit comprises a kit body, a cover plate and an operation pore plate, wherein reaction holes, a filter groove, a tweezer groove, an absorbent paper use groove and an absorbent paper storage groove are formed in the operation pore plate, and the reaction holes sequentially comprise a 40% polymethanol hole, a 70% alcohol hole, a 85% alcohol hole, a 95% alcohol hole, a 100% alcohol hole, a xylene hole and a liquid paraffin hole according to counterclockwise arrangement. The invention further provides a using method of the micro tissue paraffin embedding pretreatment kit, the kit contains various prepared soaking solutions, the liquid preparation time is shortened, and the reagent loss is reduced; the kit has the advantages of simplicity in operation, few experimental steps, short time, intact micro tissue form protection, no influence on the concentration of the soaking solution, more reliable data, market prospect and suitability for popularization.

Description

technical field [0001] The invention relates to the technical field of kits, in particular to a small tissue paraffin embedding pretreatment kit and a use method thereof. Background technique [0002] Immunostaining is the most commonly used technique for studying tissue / cell antigens, which can qualitatively, localize, and quantify antigens. Before immunostaining, tissues / cells must go through steps such as fixation, dehydration, clearing, paraffin embedding, and sectioning. For the experimental steps of fixation, dehydration, and transparency of tiny tissues (such as blastocysts and stem cell spheres, etc., with a volume of only 0.2-1.0 mm), the traditional method achieves micro-tissues in different stages by repeatedly transferring liquid with a pipette and centrifuging to remove liquid. Soaking in liquid. The traditional operation method has the following disadvantages: 1. Centrifugation and pipetting cannot completely remove the immersion liquid of tiny tissues, resul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/36G01N1/40
CPCG01N1/36G01N1/405G01N2001/366
Inventor 刘天助杨叶青杨思进罗钢
Owner THE AFFILIATED HOSPITAL OF TRADITIONAL CHINESE MEDICAL TO SOUTHWEST MEDICAL UNIV
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