Nematicidal agricultural composition containing fluopyram and Cyclobutrafluram
A technology of fluopyram and a composition, which is applied in the field of chemical control of plant diseases and can solve problems such as resistance risks
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Embodiment 1
[0017] This example provides the laboratory toxicity test of fluopyram and Cyclobutrifluram against Meloidogyne incognita.
[0018] The 2nd instar larvae of Meloidogyne incognita were prepared with sterile water into a nematode suspension with a certain concentration (about 400 / mL) for later use. Set up different concentration gradient treatments, use sterile water as the blank control, take a 96-well cell culture plate, add 150 mL of prepared medicines of different concentrations to each well, then add an equal volume of nematode suspension, and culture at 25°C in moisture, respectively. At different times after treatment, the number of survival and death of the second instar of M. incognita was checked. The nematodes were stiff and motionless as dead worms, and the nematodes were bent and wriggling as live worms. Mortality and adjusted mortality were calculated separately.
[0019] Adjusted mortality rates for each treatment were calculated from the survey data. And refer ...
Embodiment 2
[0024] This example provides a pot test of fluopyram and Cyclobutrifluram SC on the roots of cucumber seedlings damaged by Meloidogyne incognita.
[0025] The test plants are commercially available cucumber varieties Bomei 517, grown in the greenhouse until the height of the seedlings is 15-20cm, and transplanted to a height of 400cm per plant. 3 In pots with sterilized soil. The soil quality of the test soil is sandy loam, which is sterilized at 121°C for 30 minutes before use. The test soil is treated with the test agent so that every pot of 400cm 3 The doses in the sterilized soil were 0, 6.25, 15.625, 31.25, 62.5, 93.75, 125, 156.25 μg. After the cucumber seedlings were transplanted, they were cultured in the artificial greenhouse for 3 days and then used to inoculate the root-knot nematode incognita.
[0026] Test agent is self-made 400g / L fluopyram · Cyclobutrifluram suspension concentrate (the mass ratio of fluopyram and Cyclobutrifluram is 5:1), denoted as TM1; self-...
Embodiment 3
[0032] This example provides the preparation of a fluopyram·Cyclobutrifluram granule (the mass ratio of fluopyram and Cyclobutrifluram is 1:1) and its field control effect for controlling peanut root-knot nematode.
[0033] The components and proportions of Fluopyram Cyclobutrifluram granules described in this example: Fluopyram 1%, Cyclobutrifluram 1%, corncob biomass charcoal (pyrolysis temperature 680°C, pyrolysis time 2h) 15%, Sodium alginate 5%, carboxymethyl cellulose 10%, kaolin clay balance. Carboxymethyl cellulose was used as the binder, and the remaining materials were crushed through a 100-mesh sieve, granulated by a coating machine, dried in a fluidized bed at 45°C, cooled to room temperature, and sealed for storage.
[0034] The control medicines were 2% fluopyram granules and 2% Cyclobutrifluram granules, and the blank control medicines were granules without nematicide active ingredients, all prepared by the same process.
[0035] The experimental field was sele...
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