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Application of osftip7 gene and its mutants in alleviating the stress of metal oxide nanoparticles on rice

A technology of nano-particles and oxides, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems that have not been reported, and achieve the effects of improving oxidative stress, alleviating toxicity, and improving synthesis

Active Publication Date: 2022-01-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few studies on how to solve the metal oxide nanoparticle stress in rice, and the research involving rice-specific genes in response to metal oxide nanoparticle stress in rice is even less reported.

Method used

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  • Application of osftip7 gene and its mutants in alleviating the stress of metal oxide nanoparticles on rice
  • Application of osftip7 gene and its mutants in alleviating the stress of metal oxide nanoparticles on rice
  • Application of osftip7 gene and its mutants in alleviating the stress of metal oxide nanoparticles on rice

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1 Source of OsFTIP7 gene

[0040] The description, source and function of the OsFTIP7 gene in this application are recorded in the above-mentioned literature. According to the description of the literature, the OsFTIP7 gene is obtained in the following way: in NCBI, the homologous comparison of the FTIP1 (AT5G0685) gene in Arabidopsis Homologous genes in rice, a gene (Os05g0370600) containing high homology to the MCTP domain of FTIP1 was found and named OsFTIP7, which contains three C2 domains and a phosphoribosyltransferase C-terminal domain (PRT_C ).

[0041] Of course, those skilled in the art can also obtain it by artificial synthesis directly according to the accession number (the accession number of the OsFTIP7 gene in Genbank of NCBI is AK058522).

Embodiment 2

[0042] Example 2 Obtaining of Osftip7 mutants

[0043] The description, source and function of the mutant Osftip7 material used in the present invention are recorded in the above-mentioned literature. According to the description of the literature, it can be known that the mutant is obtained by applying CRISPR / Cas9 gene editing technology, specifically: first, according to the obtained OsFTIP7 gene The pCAMBIA1300-CAS9-Os-OsFTIP7 vector was constructed, the psgR-CAS9-Os vector was digested with BbsI, and ligated with the synthetic sgRNA oligos, and then the obtained fragment was ligated into the rice pCAMBIA1300 binary vector (HindIII / EcoRI), and transformed into the agricultural Bacillus, and use the rice variety Nipponbare for genetic transformation, and finally obtain mutation sites such as figure 1 The mutants shown, the above-mentioned methods and the reagents used are conventional methods and reagents in the technical field, such as the methods and reagents described in ...

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Abstract

The invention discloses the application of the OsFTIP7 gene and its mutants in alleviating the stress of metal oxide nanoparticles to rice, and relates to the technical field of crop physiology. The invention proves that the Osftip7 mutant has the ability to promote the growth of rice under the stress of metal oxide nanoparticles, Alleviate the toxicity of metal oxide nanoparticles to rice, such as increasing the chlorophyll synthesis of rice under the stress of metal oxide nanoparticles, increasing its plant height and dry weight, and improving the oxidative stress induced by metal oxide nanoparticles.

Description

technical field [0001] The invention relates to the technical field of crop physiology, in particular to the application of an OsFTIP7 gene and a mutant thereof in alleviating the stress of metal oxide nanoparticles to rice. Background technique [0002] With the rapid development of nanotechnology, people have been able to manufacture nanomaterials of different types, sizes and shapes. Due to their unique physical and chemical properties, nanoparticles have been widely used in various aspects of daily life, such as electronic communication, environmental protection, medical medicine and agricultural production, etc. [0003] As important metal oxide NPs, copper oxide (CuO) nanoparticles and zinc oxide (ZnO) nanoparticles have been applied in various fields. For example, ZnO nanoparticles can be used in cosmetics and sunscreens because of their strong ability to absorb ultraviolet rays; ZnO nanoparticles can be used in rubber industry production because they can enhance the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/54A01H5/00A01H6/46
CPCC12N9/1077C12N15/8218C12N15/8271
Inventor 宋士勇陈颖蒋萌杨丽佳
Owner ZHEJIANG UNIV