High performance liquid chromatography detection method for cyanidin and three anthocyanins in fruits and vegetables
A high-performance liquid chromatography and cyanidin technology, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to accurately analyze and detect the content of anthocyanin monomers, and the establishment of fruits and vegetables, etc. Easy to promote, simple effect
Active Publication Date: 2020-11-27
INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology
Spectrophotometry has the characteristics of requiring less equipment, low price, and simple operation, but it can only analyze the total amount of anthocyanins, and cannot accurately analyze and detect the content of anthocyanin monomers
However, the HPLC method reported at present is only suitable for detecting certain specific anthocyanins in a single fruit and vegetable. cyanidin-3-O-glucoside, cyanidin-3-glucoside and cyanidin-3-rutinoside in red peach fruit, but cyanidin and geranidin-3 in fruits and vegetables have not yet been established. , a general detection method for 5-diglucoside, cyanidin-3-O-glucoside, paeoniflorin-3-O-glucoside (its structural formula is shown in the following formula)
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[0032] 1. Detection method
[0033] 1 Reagents and equipment
[0034] Standard substances of cyanidin, geranidin-3,5-diglucoside, cyanidin-3-O-glucoside, paeoniflorin-3-O-glucoside, purity ≥95%; methanol, acetonitrile, formic acid Reagents are chromatographically pure; hydrochloric acid is superior grade.
[0035] Waters high-performance liquid chromatography E2695, visible light detector, Inert Sustain C18 chromatographic analysis column (4.6×250mm, 5μm), vortex oscillator, centrifuge, ultrasonic cleaner.
[0036] 2 Sample pretreatment
[0037] For darker-colored fruit and vegetable samples (such as mulberries, purple cabbage), weigh 2 g of the homogenate sample (accurate to 0.01 g); for lighter-colored fruit and vegetable samples (such as apples, purple onions, eggplants), weigh the homogenized sample Sample 5g (accurate to 0.01g). Place in a centrifuge tube, add 30mL of 0.5% hydrochloric acid-methanol solution, place on a vortex shaker, oscillate and vortex for 1min, an...
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The invention discloses a high performance liquid chromatography detection method for cyanidin and three anthocyanins in fruits and vegetables. The high performance liquid chromatography detection method comprises the following steps of: extracting anthocyanin (anthocyanidin) in a sample by using a hydrochloric acid-methanol mixed solution; carrying out vortex uniform mixing on the mixture, carrying out extraction for 10-15 h in a dark place; carrying out determination at a wavelength of 520 nm by using a high performance liquid chromatograph equipped with a visible light detector; carrying out qualitative analysis according to the retention time of chromatographic peaks; and carrying out quantification by using an external standard method. According to the high performance liquid chromatography detection method, cyanidin, geranium-3, 5-diglucoside, cyanidin-3-O-glucoside and peonidin-3-O-glucoside can be accurately, rapidly and simultaneously analyzed, the pretreatment method is simple, computer analysis can be carried out only through extraction, and the adopted instrument high performance liquid chromatograph is wide in application in various laboratories, easy in promotion andhigh in applicability.
Description
technical field [0001] The invention relates to a high-efficiency solution of cyanidin and three kinds of anthocyanins (geranidin-3,5-diglucoside, cyanidin-3-O-glucoside, paeoniflorin-3-O-glucoside) in fruits and vegetables A phase chromatography detection method belongs to the technical field of analysis and detection. Background technique [0002] Cyaniidin belongs to anthocyanin, while geranidin-3,5-diglucoside, cyanidin-3-O-glucoside and paeoniflorin-3-O-glucoside belong to anthocyanin compounds, which are anthocyanins A class of compounds combined with sugar by glycosidic bonds. Anthocyanins and anthocyanins are widely found in many fruit and vegetable products, such as mulberry, strawberry, blueberry, grape, purple cabbage, red radish, eggplant, purple onion, etc. Nutrition and health functions, such as anti-oxidation, anti-mutation and tumor, play a pivotal role in preventing cardiovascular diseases and promoting human health. [0003] The crude extracts of anthocya...
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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N2030/146
Inventor 蔡达王玉涛赵玉华刘俊华秦宏伟张潇
Owner INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI