Application of cyclocarya paliurus leaves or extract thereof in preparation of antibacterial agent
A technology of extracts and antibacterial agents, applied in the field of preparing antibacterial agents from leaves of Cyclocarya paliurus or its extracts
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Embodiment 1
[0028] Embodiment 1, the preparation of Cyclocarya paliurus leaf aqueous extract
[0029] Take 50g of Cyclocarya paliurus leaves (from the planting base of Guangwu Mountain, Nanjiang County, Sichuan Province, washed and stored in a dry place at room temperature), add 15 times the amount of water, and heat at 90°C for extraction twice, each time for 1.5h. The water extract was suction filtered while it was hot, cooled, and the measured volume was 1450ml to obtain the water extract of Cyclocarya paliurus leaves.
[0030] Take 250mL of the above water extract and concentrate under reduced pressure at 80°C and 65r / min, and concentrate to 60mL for later use (used in Experimental Example 1 below).
[0031] Using ultraviolet-visible spectrophotometry ("Chinese Pharmacopoeia" 2015 edition general rule 0401), with rutin as a contrast, the concentration of total flavonoids in the concentrated solution is 0.0426%; with anhydrous glucose as a contrast, the total polysaccharides in the con...
experiment example 1
[0035] Experimental example 1, the test of the bactericidal effect of the aqueous extract of Cyclocarya paliurus leaves
[0036] 1. Culture of bacteria
[0037] Culture medium: Escherichia coli ATCC25922, Escherichia coli CMCC44155, Staphylococcus aureus ATCC25923, Staphylococcus epidermidis ATCC8033, Staphylococcus saprophyticus using MH medium; Streptococcus pneumoniae ATCC6303, A-hemolytic streptococcus, B-hemolytic streptococcus The culture of Lactobacillus acidophilus ATCC4356 adopts MH medium containing 5% serum; the culture of Lactobacillus acidophilus ATCC4356 adopts MRS medium.
[0038] Culture conditions: each bacteria was cultured on a liquid shaker at 37°C for 4 hours; Streptococcus pneumoniae, Lactobacillus acidophilus and Staphylococcus saprophyticus were cultured overnight.
[0039] 2. Experimental method
[0040] Dilution of the extract: the extracts (respectively take the water extract of Cyclocarya paliurus leaves obtained in Example 1 and the water extract...
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