Temperate bacteriophage VneM1 for regulating and controlling coral flora and application of mild bacteriophage VneM1

Abstract

The invention discloses a mild bacteriophage VneM1 for regulating and controlling coral flora and application of the mild bacteriophage VneM1. The preservation number of the bacteriophage is Vibrio neocaledonicus bacteroiphage VneM1 is CGMCC NO.61215-B1. The invention also discloses a preparation method of the bacteriophage. According to the invention, the new bacteriophage-Vibrio neocaledonicus bacteriophage VneM1 is separated from a coral symbiotic bacterium Vibrio neocaledonicus SCSIO 43009. The bacteriophage VneM1 has a cracking effect on coral symbiotic vibrio, and regulates and controlsthe composition of coral symbiotic microorganisms. Hydranth infection experiments prove that the bacteriophage VneM1 can delay coral whitening caused by temperature rise. The bacteriophage VneM1 is amild bacteriophage, and under normal conditions, the bacteriophage VneM1 is integrated on an original lysozyme SCSIO 43009 genome and exists in a form of a prophage. The bacteriophage can be producedby spontaneous lysogen-lysis conversion, and the lysogen-lysis conversion can be promoted by environmental factors such as SOS stress reaction.

Description

[0001] The invention belongs to the field of marine biology, and in particular relates to a mild phage for regulating coral intestinal flora and its application. Background technique [0002] Coral reefs are a type of marine ecosystem rich in species diversity composed of corals, fish, algae and other marine animals and plants, as well as microorganisms. Coral reef ecosystems are highly productive, have important economic value, and play an important role in processes such as biogeochemical cycles. However, due to a combination of environmental pressures such as global warming and anthropogenic pressures, the productivity of coral reef ecosystems has declined dramatically over the past 30 years. The current situation of my country's coral reefs is particularly severe. The number of reef-building corals that make up the mainland and the hidden reefs of Hainan Island has dropped by 80%. According to reports, one of the important reasons for its sharp decline is the outbreak of ...

AI Technical Summary

Problems solved by technology

Phages may help corals resist disease by lysing pathogenic bacteria, such as using BA3 phage to specifically kill coral pathogenic bacteria Thalassomonas loyana , can effectively control the spread of pathogens in corals. However, due to the species-specificity of phage infection, BA3 phages cannot act on coral diseases caused by other coral pathogenic bacteria

Metamicrobiome study finds that some corals are born with beneficial phages that enhance coral resistance to disease, but these phages have not yet been identified

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