A device for in-situ cultivation and enrichment of anaerobic microorganisms in groundwater and its application method
A technology of anaerobic microorganisms and microbial incubators, which is applied in the field of in-situ cultivation and enrichment devices for anaerobic microorganisms in groundwater, can solve the problems of small quantity, changes in the structure characteristics of anaerobic microbial communities, and low microbial elution efficiency, and achieve structural Simple, economical and practical, structurally stable effect
Active Publication Date: 2021-03-23
北京博瑞兴环境科技有限公司
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Problems solved by technology
[0007] The purpose of the present invention is to provide a device and method for in-situ cultivation and enrichment of anaerobic microorganisms in groundwater. It is necessary to solve the problems of easy exposure of collected microbial samples, low microbial elution efficiency, and inability to ensure a strict anaerobic environment so that the anaerobic microbial community Structural characteristics change, the number of samples sent to the laboratory is small, and the technical problem of unable to reach the microbial load required for the expansion of the library
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Embodiment 1
[0060] The present invention provides an in-situ cultivation and enrichment device for anaerobic microorganisms in groundwater. The enrichment device comprises an airtight manhole cover 4 arranged on the top surface of a groundwater monitoring well, a water sealer 1 arranged in the groundwater monitoring well, and a Microbial cultivator 2 and fixed rope in water sealer 1, described water sealer 1 is cylinder, water sealer 1 comprises water sealer main body 11, water sealer top cover 13 and water sealer bottom cover 12, can Adopt polytetrafluoroethylene, the top cover 13 of the water sealer is provided with a skirt, the top cover 13 of the water sealer and the upper part of the main body 11 of the water sealer are connected by threads, and the two sides of the skirt are provided with rope fixing rings; the skirt is A circle protruding toward the center of the circle blocks the incubator when the incubator is lifted up, so that the incubator will not be directly lifted out of the...
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Abstract
The invention discloses an in-situ culture enrichment device for anaerobic microorganisms in underground water and a use method. The enrichment device comprises an airtight well lid arranged on the top surface of an underground water monitoring well, a water sealer arranged in the underground water monitoring well, a microorganism incubator arranged in the water sealer and a fixing rope, wherein the water sealer is cylindrical and comprises a water sealer main body, a water sealer top cover and a water sealer bottom cover; a skirt edge is arranged on the water sealer top cover; the water sealer top cover is in threaded connection with the upper part of the water sealer main body; rope fixing rings are arranged on two sides of the skirt edge; and a one-way valve is arranged in the center ofthe water sealer bottom cover and is in threaded connection with the water sealer main body. The microorganism elution efficiency is high, an anaerobic environment can be kept all the time, so that an anaerobic microorganism community structure is stable, a large number of samples are fed into a laboratory, and an experiment of amplification and database construction can be smoothly completed.
Description
technical field [0001] The invention relates to the field of environmental engineering, in particular to a device for in-situ cultivation and enrichment of anaerobic microorganisms in groundwater and a method for using it. Background technique [0002] Benzene, petroleum hydrocarbons, chlorinated aliphatic hydrocarbons and other organic substances are one of the characteristic pollutants in groundwater in my country, especially in the organic chemical industry groundwater pollution is particularly serious. [0003] For organic matter in groundwater, common treatment methods include extraction treatment, chemical oxidation / reduction, gas phase extraction, etc. Affected by factors such as environmental pollution and other factors, the above-mentioned engineering restoration measures all have a certain degree of tailing phenomenon. During the tailing period, the concentration of pollutants decreases very slowly, and the marginal cost of facility operation gradually increases. ...
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IPC IPC(8): C12M1/00C12M1/12
CPCC12M23/02C12M23/38C12M29/04
Inventor 肖庆文沙涛阚兴艳
Owner 北京博瑞兴环境科技有限公司