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Method for determining influence of pollutants on content of active oxygen in green alga cells

A technology of active oxygen content and pollutants, applied in the field of environmental toxicology, can solve problems such as oxidative damage and cell death, and achieve the effects of accurate judgment, simple operation and strong practicability

Pending Publication Date: 2020-12-04
GUILIN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

Reactive oxygen species (ROS) are very important to organisms, but excessive amounts will cause oxidative damage to organisms and lead to cell death

Method used

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  • Method for determining influence of pollutants on content of active oxygen in green alga cells
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  • Method for determining influence of pollutants on content of active oxygen in green alga cells

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Embodiment

[0028] 1.1 Preparation of related drugs

[0029] 1.1.1 Preparation of BG-II medium: BG-II medium is used as the medium for green algae, and its formula is shown in Table 1. Prepare the BG-II medium formula according to the formula in Table 1. The preparation method is: prepare the ingredients in Table 1 to make the corresponding mother liquor concentration, and transfer the corresponding mother liquor dosage to a 1000mL volumetric flask in the order indicated, seal and paste Label well, and dilute to 1000mL with distilled water, sterilize at 121°C and 101KPa for 20 minutes, after sterilization and cooling, store in a refrigerator at 4°C, valid for 2 months.

[0030] Table 1 BG-II medium formula

[0031]

[0032] NOTE: Adjust the pH to 7.1 with 1M NaOH or HCl.

[0033] 1.1.2 Configuration of the mother solution of the test substance pesticide azole fungicide: Weigh a certain amount of the original drug of the pollutant, put it into a small beaker and add BG-II medium to di...

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Abstract

The invention discloses a method for determining the influence of pollutants on the content of active oxygen in green alga cells. Chlorella pyrenoidosa is used for evaluating the influence of a typical pollutant azole bactericide (clotrimazole) on the content of active oxygen in green alga cells; and evaluating the influence of pollutants on active oxygen in the green alga cells according to the DCF fluorescence intensity of the single green alga cells under the action of the azole bactericide pesticide. According to the method, the detection result of the change of the content of the active oxygen in the green alga cells is more accurate, and the problem that the fluorescence intensity measurement is inaccurate due to the change of the number of the cells caused by exposure of pollutantsat different concentrations is solved. The fluorescence intensity of DCF is in direct proportion to the content of active oxygen in cells, so that the influence of pollutants on the content of activeoxygen in cells under a certain concentration can be reflected more intuitively. The method is simple, convenient and rapid in test, low in detection system background, high in sensitivity and good inrepeatability.

Description

technical field [0001] The invention belongs to the field of environmental toxicology, in particular to a method for measuring the influence of pollutants on the active oxygen content in green algae cells. The method uses flow cytometry to detect the effect of pesticide pollutant azole fungicide (clotrimazole) on Chlorella pyrenoidosa ( Chlorella pyrenoidosa ) The effect of intracellular active oxygen content. Background technique [0002] Chlorella is the primary producer of aquatic ecosystems, plays an extremely important role in the balance and stability of water bodies, and is an important indicator reflecting the environmental quality of water bodies. Reactive oxygen species (ROS) are very important to organisms, but excessive amounts can cause oxidative damage to organisms and lead to cell death. Due to the short lifespan and high reactivity of ROS, and most of them exist in the body and are difficult to capture, the direct detection of ROS in vivo is of great signif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N21/64C12Q1/02C12R1/89
CPCG01N15/1404G01N21/6428G01N21/6486C12Q1/025G01N2015/1006
Inventor 刘敏覃礼堂莫凌云梁延鹏曾鸿鹄宋晓红
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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