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A strain of chlamydia pulcinia for controlling root-knot nematode disease and its compound powder and its application

A root-knot nematode and powder technology, applied in nematicide, application, pest control and other directions, can solve the problems of water source, soil and crop pollution, affecting human health, and pest resistance, etc., to promote crops. The effect of growth, protection of ecological sustainable development and protection of diversity

Active Publication Date: 2022-07-05
青岛和协生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most nematocides currently used in production are highly toxic chemical pesticides, which not only affect human health, but also pose a threat to the environment
Long-term use of a large amount of pesticides will easily cause pesticide resistance of pests, soil compaction, pollution of water sources, soil and crops, and decline in the quality of agricultural products

Method used

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  • A strain of chlamydia pulcinia for controlling root-knot nematode disease and its compound powder and its application
  • A strain of chlamydia pulcinia for controlling root-knot nematode disease and its compound powder and its application
  • A strain of chlamydia pulcinia for controlling root-knot nematode disease and its compound powder and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Isolation and Screening of Puccinia pachychinensis HX413

[0031] About 500 g of root knot nematodes were collected from the soil of a tomato planting greenhouse in Jiangjiazhuang Village, Bolan Town, Jimo District, Qingdao City, Shandong Province, and about 500 g of tomato roots were infested. In a funnel of water, connect a rubber tube to the bottom of the funnel and drain it to the nematode receiving test tube. After 1 day, clamp the rubber tube, take out the receiving test tube, centrifuge at 6000 rpm, discard the supernatant to obtain 1.5 ml of nematode suspension; take 0.3 ml of nematode suspension The turbid liquid was coated on a 2% agar horizontal plate, and cultivated at 26-28°C for 15-20 days; when colonies formed, a small amount of mycelium was picked from the edge of the colony and moved to a new PDA plate, cultivated for 7 days, and the colony morphology was observed. Carry out preliminary identification, and transfer the colony to CMA plate for purificati...

Embodiment 2

[0034] According to the formula in Table 1, microbial protection agents 1-3 were prepared.

[0035] Among them, zeolite powder, humic acid, corn starch, sucrose, sodium chloride and soil temperature are all commercial products. Wherein, the carbohydrate or protein substance is instant soybean powder.

[0036] Table 1

[0037] Preparation example Zeolite powder (g) Humic acid (g) Corn starch (g) Sucrose (g) Sodium chloride (g) soil temperature (g) 1 30 10 20 0.5 0.1 0.1 2 45 20 30 1.2 1 1 3 50 30 40 2 1.5 1.5

Embodiment 3

[0039] A kind of compound microbial powder containing chlamydia thaliana for controlling root-knot nematode disease

[0040] According to the formula in Table 2, composite microorganism powders 1-4 were prepared, wherein the carbohydrate or protein substance was instant soybean powder, and the protective agent was the protective agent 1 obtained in Example 2.

[0041] Table 2

[0042]

[0043] A kind of preparation method of the compound microorganism powder that contains the chlamydia thaliana for preventing and treating root-knot nematode disease comprises the steps:

[0044] Mix the hyphae of Chlamydomonas thaliana HX413 obtained by self-cultivation and isolation with the purchased Paecilomyces lilacinus powder, protective agent and instant soybean powder, adjust the moisture to 15-20%, and then dry at below 30°C. It is obtained until the moisture is below 8%.

[0045] Table 3 shows the content of Paecilomyces lilacinus and Chlamydomonas pachypuscinia HX413 of the obta...

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Abstract

The invention discloses a strain of chlamydia for preventing and treating root-knot nematode disease, a compound powder and application thereof, and belongs to the technical field of microbial inoculants. The plucynia pluvialis is the strain HX413 of pulvinia pluvialis, which has been deposited in the General Microbiology Center of the China Microbial Culture Collection and Management Committee on June 17, 2020. The preservation number is: CGMCC: 20207, and the classification is named. : Pochonia chlamydosporia. The Puccinia thaliana used in the invention has excellent performance in preventing and treating root-knot nematode diseases, and is compounded with the traditional nematicidal fungus Paecilomyces lilacinus, and the two bacteria play a synergistic effect and have a nematicidal effect. It is greatly improved, and at the same time has the functions of nitrogen fixation, fertilization, root mulching and crop growth promotion. The compound microbial powder acts on crops in agricultural production to increase crop yield, and the yield increase rate is over 22%.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculants, and in particular relates to a strain of Chlamydomonas puccinia for preventing and treating root-knot nematode disease, a composite powder and application thereof. Background technique [0002] Root-knot nematode is a highly specialized omnivorous plant pathogenic nematode. After continuous cropping, root-knot nematode produces a large number of eggs in plant roots and hatches, resulting in large-scale crop yield reduction. Root-knot nematode disease leads to a reduction in food production and a huge loss of economic income worldwide every year. [0003] Chlamydomonas thaliana is an endoparasitic fungus that can parasitize eggs, larvae, females, etc., and can significantly reduce the damage of plant nematodes such as root-knot nematodes, cyst nematodes, and stem nematodes of various crops. Puccinia thaliana can not only inhibit the infection of nematodes, but also promote the growth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P5/00A01P21/00C12R1/645C12R1/79
CPCC12N1/14A01N63/30C12R2001/645C12N1/145Y02A40/10
Inventor 宗睿梁寒冰刘鲁民倪海平
Owner 青岛和协生物科技有限公司
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