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System and method for researching immunosenescence mechanism of HIV infected person

An immunosenescence and infection technology, applied in the field of medical research, can solve the problem that the immune aging process of HIV-infected patients cannot be explained, the immune aging mechanism and process of HIV-infected patients cannot be completely and accurately reflected, and the correlation of multiple indicators is not considered at the same time. Sexuality and the degree of mutual influence, etc., to achieve the effect of benefiting immune function and reconstruction

Active Publication Date: 2020-12-11
成都市疾病预防控制中心
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two indicators cannot explain the immune aging process in HIV-infected patients
[0004] In the existing technology, the research model on the immune aging mechanism of HIV-infected patients is still in the comparison and research of a single index, without considering the correlation and mutual influence between multiple indexes in the three dimensions of nucleic acid, cells, and cytokines at the same time , so the analysis results of the established research model cannot completely and accurately reflect the immune aging mechanism and process of HIV-infected patients

Method used

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  • System and method for researching immunosenescence mechanism of HIV infected person
  • System and method for researching immunosenescence mechanism of HIV infected person
  • System and method for researching immunosenescence mechanism of HIV infected person

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Such as figure 1 A system shown for studying mechanisms of immune aging in HIV-infected individuals includes:

[0058] A sample collection unit for obtaining whole blood samples and plasma samples from HIV-infected persons;

[0059] The first detection unit is used to detect the content of the HIV-1 DNA virus library in the whole blood sample to obtain HIV-1 DNA detection data;

[0060] The second detection unit is used to detect the content of T lymphocyte subsets related to immune aging, and obtain the detection data of T lymphocyte subsets;

[0061] The third detection unit is used to detect the content of cytokines related to body inflammation and homeostasis in the plasma sample, and obtain cytokine detection data;

[0062] The analysis unit is used to receive and analyze the correlation and degree of mutual influence among HIV-1 DNA detection data, T lymphocyte subset detection data and cytokine detection data.

[0063] In some embodiments, the first detection un...

Embodiment 2

[0067] On the basis of Example 1, the first detection unit includes a quantitative standard curve drawing unit, and the quantitative standard curve drawing unit formulates a double-gene quantitative standard curve based on the internal reference gene of the human-specific gene fragment and the specific gene of HIV-1 DNA for PCR quantitative detection.

[0068] In one or more embodiments, the first detection unit includes a validity verification unit for double verification of the qualitative result. In one embodiment, the validity verification unit is also used to verify the validity of the kit and / or the validity of the sample.

Embodiment 3

[0070] On the basis of the above examples, the T lymphocyte subsets related to immune senescence include naive T lymphocytes, central memory T lymphocytes, effector memory T lymphocytes, activated T lymphocytes, senescent T lymphocytes, and aging T lymphocytes. one or more of cells, exhausted T lymphocytes, and thymus-exported T lymphocytes. Selecting T lymphocyte subsets as the research object, including cell subsets representing abnormal differentiation, overactivation, overaging, overaging, overexhaustion and weakened thymic output function, can more sensitively and comprehensively reflect the immune aging of T lymphocytes Case.

[0071] In one or more embodiments, the second detection unit uses a monoclonal antibody fluorescent dye to stain T lymphocyte subsets, wherein the fluorescent monoclonal antibody of naive T lymphocytes is CD3-PC7, CD4-FITC, CD8-PC5 .5. CD45RA-PE, CD27-ECD; fluorescent monoclonal antibodies of central memory T lymphocytes and effector memory T lym...

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Abstract

The invention relates to a system and method for researching an immunosenescence mechanism of an HIV infected person. The system comprises a sample collection unit which is used for obtaining a wholeblood sample and plasma sample of the HIV infected person, a first detection unit which is used for detecting the content of a HIV-1DNA virus library in the whole blood sample, a second detection unitwhich is used for detecting the content of T lymphocyte subsets related to immunosenescence, a third detection unit which is used for detecting the content of cytokines related to body inflammation and homeostasis in the plasma sample, and an analysis unit which is used for receiving and analyzing the correlation and mutual influence degree among the HIV-1DNA, the T lymphocyte subsets and the cytokines. The detection system and method covering nucleic acid, cells and cytokines are established, and the mechanism and process of immunosenescence of the HIV infected person are reflected more completely from the whole in combination with statistical analysis, so that the objective scientific support is provided for the research and development of HIV medicines and the evaluation of medicationeffects or effects of other intervention means, and the reconstruction of the immune function of the HIV infected person is also facilitated.

Description

technical field [0001] The invention relates to the field of medical research, in particular to a system and method for studying the immune aging mechanism of HIV-infected persons. Background technique [0002] With the development of medical level, the application of high-efficiency antiretroviral therapy (Antiretroviral therapy, ART) can effectively inhibit the replication of HIV (Human Immunodeficiency Virus, HIV), greatly reducing the morbidity and mortality of AIDS, but it cannot completely eliminate The latent HIV virus in the body. Long-term chronic viral infection can damage the normal immune function of the body, trigger chronic systemic inflammation, and lead to the occurrence of immunosenescence (Immunosenescence). Unlike immune degeneration, immune senescence is an adaptive process of continuous depletion and remodeling caused by chronic antigenic load. Immune senescence is not unique to HIV, and other viral chronic infections such as CMV and middle-aged and el...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851C12Q1/70G01N33/68G01N33/58G01N33/543G01N15/14
CPCC12Q1/6851C12Q1/703G01N15/14G01N33/543G01N33/581G01N33/6863G01N33/6869G01N2015/1006G01N2333/525G01N2333/5412G01N2333/5418G01N2800/7095C12Q2531/113C12Q2563/107C12Q2545/101
Inventor 郭悦张燕琳李姗珊
Owner 成都市疾病预防控制中心
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