Extraction method of 7-OH- mitragynine

A technology of mitragynine and extraction method, which is applied in the field of medicine, can solve the problems of cumbersome steps, low purity and yield, and long time consumption, and achieve the effects of high extraction rate and purity, simple extraction method, and simple operation

Active Publication Date: 2020-12-18
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the existing literature adopts the soxhlet extraction method, using methanol as a solvent to extract the crude extract from kratom leaves, but these methods are inefficient and time-consuming, requiring several days to obtain the crude extract; and the obtained crude extract contains more The impurity is sticky, and it needs to go through a series of steps such as extraction, acidification, and neutralization to obtain relatively pure 7-OH-mitragynine. The steps are cumbersome, and the purity and yield are low

Method used

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Embodiment 1

[0034]This embodiment provides a method for extracting 7-OH-mitragynine, a traditional Chinese medicine composition containing 7-OH-mitragynine, and a health belt containing the traditional Chinese medicine composition; wherein,

[0035]The extraction method of 7-OH-mitragynine includes the following steps:

[0036](a) Crush the dried kratom leaves, add chloroform according to the material-to-liquid ratio of 1:10, adjust the pH to 6.11 with a mass fraction of 10% acetic acid solution, and extract the supernatant by ultrasonic extraction at room temperature for 45 minutes. This process was repeated 3 times, and the supernatant was concentrated under reduced pressure for 3 times to obtain the first concentrated solution; wherein the ultrasonic power was 200W;

[0037](b) Add an equal volume of petroleum ether to the first concentrated solution and extract at 50°C. After standing still, remove the lower layer liquid. This process is repeated 3 times, the lower layer liquid is combined 3 times, ...

Embodiment 2

[0045]This embodiment provides a method for extracting 7-OH-mitragynine, a traditional Chinese medicine composition containing 7-OH-mitragynine, and a health belt containing the traditional Chinese medicine composition; wherein,

[0046]The extraction method of 7-OH-mitragynine includes the following steps:

[0047](a) Crush the dried kratom leaves, add chloroform according to the material-to-liquid ratio of 1:12, adjust the pH value to 6.11 with a mass fraction of 10% acetic acid solution, ultrasonically extract for 1.5h at room temperature, and centrifuge to get the supernatant , This process was repeated 3 times, and the supernatant was concentrated under reduced pressure for 3 times to obtain the first concentrated solution; where the ultrasonic power was 200W;

[0048](b) Add an equal volume of petroleum ether to the first concentrated solution and extract at 40°C. After standing still, remove the lower layer liquid. This process is repeated 3 times, the lower layer liquid is combined 3...

Embodiment 3

[0054]This embodiment provides a method for extracting 7-OH-mitragynine, a traditional Chinese medicine composition containing 7-OH-mitragynine, and a health belt containing the traditional Chinese medicine composition; wherein,

[0055]The extraction method of 7-OH-mitragynine includes the following steps:

[0056](a) Crush the dried kratom leaves, add chloroform according to the material-to-liquid ratio of 1:15, adjust the pH to 6.28 with a mass fraction of 10% acetic acid solution, and extract the supernatant by ultrasonic extraction at room temperature for 30 minutes. This process was repeated 3 times, and the supernatant was concentrated under reduced pressure for 3 times to obtain the first concentrated solution; wherein the ultrasonic power was 400W;

[0057](b) Add an equal volume of petroleum ether to the first concentrated solution and extract at 45°C. After standing still, remove the lower layer liquid. This process is repeated 3 times, the lower layer liquid is combined 3 times, ...

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Abstract

The invention discloses an extraction method of 7-OH -mitragynine, which comprises the following steps: (1) pulverizing dried kratom leaves, adding an extractant, carrying out ultrasonic extraction atnormal temperature, and carrying out centrifugation and vacuum concentration to obtain a first concentrated solution; (2) adding an extraction agent into the first concentrated solution for extraction, and performing vacuum concentration to obtain a second concentrated solution; and (3) carrying out silica gel column chromatography separation on the second concentrated solution, and carrying outgradient elution by using an eluent to obtain the 7-OH -mitragynine. The extraction method of the 7-OH -mitragynine is simple and rapid to operate, the extraction rate of the obtained 7-OH -mitragynine is 0.24%, the purity of the obtained 7-OH -mitragynine is greater than 90%, and the 7-OH -mitragyninehas higher extraction rate and purity.

Description

Technical field[0001]The present invention relates to the technical field of medicine, in particular to an extraction method of 7-OH-mitragynine.Background technique[0002]Kratom is a medicinal plant of the genus Migra that grows in Thailand, Malaysia and other Southeast Asian countries, and it is also found in Yunnan, my country at an altitude of 1,000 meters or more. As early as the late 1800s, its leaves were used to treat muscle pain, diarrhea, and coughing. It was also used to reduce the intake of expensive opioids and to treat the withdrawal response caused by opiate addiction. In addition, Malaysians and Thais also eat this plant to enhance their endurance to work hard under the scorching sun. The main active ingredient of Kratom is indole alkaloids, of which mitragynine accounts for more than 66% of the total alkaloids. 7-OH-mitragynine is a new alkaloid isolated in recent decades. Studies have found that it has stronger lipophilicity than mitragynine, easily penetrates the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D459/00
CPCC07D459/00
Inventor 徐丛玥乐凯
Owner JIANGHAN UNIVERSITY
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