Thin-layer fluorescence focusing method for rapidly detecting single-stranded nucleic acid

Abstract

The invention relates to a thin-layer fluorescence focusing method for rapidly detecting single-stranded nucleic acid. The thin-layer fluorescence focusing method comprises the following steps that: 1, a capture probe is connected with a solid-phase carrier, herein the solid-phase carrier includes but not limited to magnetic particles, a polystyrene surface, a polylysine surface and a carboxyl modified surface, and the connection includes non-specific adsorption and covalent coupling; step 2, the probe is coupled with chromogenic microspheres, herein the chromogenic microspheres include but are not limited to fluorescent microspheres with various colors, colored microspheres and the like; and step 3, a detection reaction and a signal are read in the reaction card. Compared with the prior art, the method has the advantages that RNA or DNA can be detected within 30 minutes, the sensitivity reaches 1000copies per sample, detection can be performed on site without a central laboratory, anda tool can be provided for detecting pathogens causing outburst public health events.

Description

technical field [0001] The invention relates to the technical field of single-stranded nucleic acid detection, in particular to a thin-layer fluorescence focusing method for rapidly detecting single-stranded nucleic acid. Background technique [0002] When responding to sudden infectious disease public health events, timely and rapid pathogen detection is more important to control the outbreak of infectious diseases. Since the beginning of 2020, the outbreak of the novel coronavirus (COVID-19) around the world urgently requires rapid and accurate detection methods. The standard method for the detection of the new coronavirus is to use respiratory secretions or blood samples as samples, and use real-time RT-PCR, constant temperature amplification, or genome sequencing methods for detection. The specificity of real-time RT-PCR test nucleic acid is relatively high, but the sensitivity of clinical manifestations is low, about 30%, and often requires multiple tests to confirm or...

AI Technical Summary

Problems solved by technology

Serum IgM and IgG concentrations can be detected by serological methods such as colloidal gold immunochromatography and enzyme-linked immunoassay (ELISA). The IgM concentration can reflect the current infection situation, but its sensitivity and specificity are low

Images