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Preparation method of egg yolk antibody for preventing and treating vibrio vulnificus infection

A technology of yolk antibody and Vibrio vulnificus, which is applied in chemical instruments and methods, antibodies, antibacterial drugs, etc., can solve problems such as high cost, difficulty in large-scale industrial production and application, and complex process of specific IgG. Use a wide range of effects

Inactive Publication Date: 2021-01-05
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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AI Technical Summary

Problems solved by technology

However, the extraction and preparation of specific IgG from mammalian serum is complicated and costly, making it difficult to achieve large-scale industrial production and application

Method used

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Embodiment

[0032] A yolk antibody for preventing and treating Vibrio vulnificus infection, prepared by the following method:

[0033] 1. Extraction, identification, isolation, purification and culture preservation of clinically highly pathogenic Vibrio vulnificus strains:

[0034] Patients with clinical Vibrio vulnificus infection were selected, and their wound vesicle fluid or tissue exudate was collected. Plates were plated in blood plates supplemented with 0.5% sodium chloride. Place in a constant temperature incubator at 37°C overnight. After a single colony was picked and purified by centrifugation, a mass spectrometer was used to determine whether it was Vibrio vulnificus infection. After the diagnosis, the bacteria were cultured and amplified, and stored for future use.

[0035] 2. Preparation of Vibrio vulnificus inactivated bacterial antigen:

[0036] Vibrio vulnificus was inactivated by formaldehyde inactivation method to prepare total inactivated bacterial antigen. Multip...

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PUM

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Abstract

The invention provides an egg yolk antibody for preventing and treating vibrio vulnificus infection. The egg yolk antibody is prepared by the following method: (1) separating vibrio vulnificus from asample of a patient infected with the vibrio vulnificus in natural environment; (2) preparing a vibrio vulnificus inactivation somatic antigen, and carrying out immunization on an egg-laying hen; and(3) collecting immunized eggs, and extracting and purifying the egg yolk antibody from the egg yolks of the eggs. The egg yolk antibody disclosed by the invention can effectively neutralize the vibriovulnificus so as to terminate the vibrio vulnificus infection, and an effective passive immune agent for subsequent prevention and control of the vibrio vulnificus is provided.

Description

technical field [0001] The invention belongs to the technical field of biological product production, in particular to a method for preparing yolk antibody for preventing and treating Vibrio vulnificus infection. Background technique [0002] With the continuous progress of social economy, human beings have an increasing demand for various resources. The ocean contains extremely rich resources, prompting people to gradually turn their attention to the ocean. As countries pay more attention to marine resources and shipping, human marine economic activities are becoming more and more frequent. The subsequent incidence of marine-related infectious diseases is also increasing year by year. [0003] Vibrio vulnificus is a deadly marine Vibrio. The rate of amputation and death remains high. Current treatment methods, including traditional wound debridement and the use of antibiotics, cannot effectively reduce the risk of death. Vibrio has the highest detection rate in marine ...

Claims

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Application Information

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IPC IPC(8): C07K16/12C07K16/02A61K39/40A61P31/04
CPCA61P31/04C07K16/02C07K16/1239C07K2317/76
Inventor 祁少海蔡瑞昭舒斌
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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