Preparation method and application of ultrahigh-temperature aerobic composite biological bacterium agent
A technology of compound microbial bacteria and biological inoculants, which is applied in the field of preparation of ultra-high temperature aerobic composite biological inoculants, can solve the problems of reducing the effect of fermentation and decomposition, loss of activity, etc. The effect of enzymatic reaction efficiency
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Embodiment 1
[0035] Embodiment 1 A kind of super high temperature aerobic composite biological bacterial agent
[0036] The ultra-high temperature aerobic composite biological bacterial agent is composed of the following components and parts by weight: 50 parts of high temperature resistant composite microbial bacterial powder, 15 parts of composite carrier, 5 parts of adsorbent and 3 parts of decomposition accelerator; The composite carrier is composed of wood chips and corn flour in a weight ratio of 1:1.
[0037] The preparation method of the high temperature-resistant compound microbial bacterial powder is as follows:
[0038] S1. Activation culture: respectively inoculate Geobacillus thermophile, thermophilic facultative bacteria, and extreme thermophile into LB medium, and activate and cultivate at 60°C for 10 hours to obtain activated strains;
[0039] S2. Inoculate the activated thermophilic Geobacillus strain, thermophilic facultative bacteria, and extreme thermophilic bacteria i...
Embodiment 2
[0051] Embodiment 2 A kind of super high temperature aerobic composite biological bacterial agent
[0052] The ultra-high temperature aerobic composite biological bacterial agent is composed of the following components and parts by weight: 55 parts of high temperature resistant composite microbial bacterial powder, 25 parts of composite carrier, 10 parts of adsorbent and 8 parts of decomposition accelerator; The composite carrier is composed of sawdust, corn flour, rice husk and soybean flour in a weight ratio of 1:1:1:1.
[0053] The preparation method of the high temperature-resistant compound microbial bacterial powder is as follows:
[0054] S1. Activation culture: Inoculate Geobacillus thermophiles, thermophilic facultative bacteria, and extreme thermophiles into LB medium respectively, and activate and cultivate them at 70°C for 30 hours to obtain activated strains;
[0055] S2. Inoculate the activated thermophilic Geobacillus strains, thermophilic facultative bacteria,...
Embodiment 3
[0067] Embodiment 3 A kind of super high temperature aerobic compound biological bacteria agent
[0068] The ultra-high temperature aerobic composite biological bacterial agent is composed of the following components and parts by weight: 60 parts of high temperature resistant composite microbial bacterial powder, 30 parts of composite carrier, 12 parts of adsorbent and 10 parts of decomposition accelerator; The composite carrier is composed of sawdust, corn flour and rice husk in a weight ratio of 1:1:1.
[0069] The preparation method of the high temperature-resistant compound microbial bacterial powder is as follows:
[0070] S1. Activation culture: Inoculate Geobacillus thermophiles, thermophilic facultative bacteria, and extreme thermophiles into LB medium respectively, and activate and culture at 75°C for 36 hours to obtain activated strains;
[0071] S2. Inoculate the activated thermophilic Geobacillus strains, thermophilic facultative bacteria, and extreme thermophiles...
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