A kind of fish skin collagen polypeptide and its preparation method and application

A technology of collagen polypeptide and fish skin, which is applied in the field of preparation of fish skin collagen polypeptide, can solve the problems of long enzymatic hydrolysis time, unsatisfactory adsorption effect of composite activated carbon, and unsuitability for large-scale production, so as to improve enzyme activity and safety High performance and good application prospect

Active Publication Date: 2021-12-03
SOUTH CHINA UNIV OF TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This preparation method combines enzymatic hydrolysis and ultrafiltration separation technology, which can artificially control the reaction conditions and obtain products with better physical and chemical properties, but the adsorption effect of composite activated carbon is not ideal, and the product has a certain bitter taste
Patent 201810408287.X improves on this basis, selects collagenase and flavor protease for proteolysis, and uses modified activated carbon for adsorption. This method improves the adsorption efficiency and reduces the bitterness of the product, but the enzymatic hydrolysis takes a long time, which is not suitable for mass production
In addition, most of the research on the activity of collagen peptides is antioxidative activity, but there are still relatively few methods for preparing anti-osteoporotic activity.

Method used

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  • A kind of fish skin collagen polypeptide and its preparation method and application
  • A kind of fish skin collagen polypeptide and its preparation method and application
  • A kind of fish skin collagen polypeptide and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Put egg shells in 20% acetic acid solution, soak for 24 hours, remove the egg shells, rinse with ultrapure water until neutral to obtain eggshell membranes (ESM), and place them in pH 7.4 phosphate buffer at 4°C Save in;

[0032] (2) Weigh a certain amount of ESM prepared in step (1), shake and soak it in 0.75% genipin for 3 hours, wash off the excess genipin on the ESM with ultrapure water, and shake it in a constant temperature water bath at 45°C , add 5 mg / mL BAP solution, wash with PBS after cross-linking for 2 hours, and obtain ESM-BAP;

[0033] (3) Place tilapia skin collagen in 20mmol / L Na 2 CO 3 -NaHCO 3 In the buffer system, add the ESM-BAP prepared in step (2), adjust the pH value to 9.5, and the enzymatic hydrolysis temperature is 55°C. After the enzymatic hydrolysis is completed, inactivate the enzyme at 100°C for 10 minutes, and centrifuge to take the supernatant;

[0034] (4) The supernatant obtained in step (3) is subjected to ultrafiltration sep...

Embodiment 2

[0040] In step (2), 0.1, 0.25, 0.5, and 1% genipin were used respectively, and the rest of the conditions were consistent with Example 1.

Embodiment 3

[0042] In step (2), the constant temperature water bath oscillation conditions are 25° C., 35° C., 45° C., 55° C., and 65° C., and the rest of the conditions are consistent with Example 1.

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PUM

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Abstract

The invention belongs to the field of food biotechnology, and discloses a fish skin collagen polypeptide and its preparation method and application, comprising the following preparation steps: (1) immobilizing Bacillus subtilis alkaline protease (BAP) with egg shell membrane (ESM) as a carrier , to obtain ESM‑BAP; (2) Utilizing ESM‑BAP to enzymatically hydrolyze fish skin collagen and separate it through a 1-5kDa ultrafiltration membrane, and to screen and verify through human bone marrow mesenchymal stem cell experiments and zebrafish animal experiments, The fish skin collagen polypeptide with high anti-osteoporosis activity is obtained. The raw material of the fish skin collagen polypeptide obtained in the present invention is derived from fish processing waste, has high safety without any side effects, greatly increases the value of fish by-products, and at the same time reduces environmental pollution to a certain extent. The obtained collagen polypeptide is safe and reliable, has high anti-osteoporosis activity, and can be used in various fields such as food and medical treatment.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a preparation method of fish skin collagen polypeptide with anti-osteoporosis activity. Background technique [0002] According to statistics, at least 200 million people around the world suffer from osteoporosis and related diseases, mainly including the elderly and postmenopausal women. The root cause of osteoporosis is that old age, disease, drugs and other factors lead to abnormal bone metabolism in the body, increased bone resorption, decreased bone mass, and abnormal changes in bone microstructure, resulting in increased bone fragility and easy fractures. Therefore, the key to treating osteoporosis is to inhibit bone resorption and promote bone formation. In addition to drug treatment, a reasonable diet is also the key to the treatment of osteoporosis. Studies have shown that collagen peptides can be used as food supplements to improve low bone density diseases by stimul...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34C12N11/02A23L33/18A61K38/39A61P19/10
CPCA23V2002/00A61K38/00A23L33/18A61P19/10C07K14/78C12N9/54C12N11/02C12P21/06A23V2200/306A23V2250/55
Inventor 肖凯军吴圆月叶芊芊朱良
Owner SOUTH CHINA UNIV OF TECH
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