A kind of fish skin collagen polypeptide and its preparation method and application
A technology of collagen polypeptide and fish skin, which is applied in the field of preparation of fish skin collagen polypeptide, can solve the problems of long enzymatic hydrolysis time, unsatisfactory adsorption effect of composite activated carbon, and unsuitability for large-scale production, so as to improve enzyme activity and safety High performance and good application prospect
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Embodiment 1
[0031] (1) Put egg shells in 20% acetic acid solution, soak for 24 hours, remove the egg shells, rinse with ultrapure water until neutral to obtain eggshell membranes (ESM), and place them in pH 7.4 phosphate buffer at 4°C Save in;
[0032] (2) Weigh a certain amount of ESM prepared in step (1), shake and soak it in 0.75% genipin for 3 hours, wash off the excess genipin on the ESM with ultrapure water, and shake it in a constant temperature water bath at 45°C , add 5 mg / mL BAP solution, wash with PBS after cross-linking for 2 hours, and obtain ESM-BAP;
[0033] (3) Place tilapia skin collagen in 20mmol / L Na 2 CO 3 -NaHCO 3 In the buffer system, add the ESM-BAP prepared in step (2), adjust the pH value to 9.5, and the enzymatic hydrolysis temperature is 55°C. After the enzymatic hydrolysis is completed, inactivate the enzyme at 100°C for 10 minutes, and centrifuge to take the supernatant;
[0034] (4) The supernatant obtained in step (3) is subjected to ultrafiltration sep...
Embodiment 2
[0040] In step (2), 0.1, 0.25, 0.5, and 1% genipin were used respectively, and the rest of the conditions were consistent with Example 1.
Embodiment 3
[0042] In step (2), the constant temperature water bath oscillation conditions are 25° C., 35° C., 45° C., 55° C., and 65° C., and the rest of the conditions are consistent with Example 1.
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