30results about How to "Maintain affinity" patented technology

A method for controlling communications between a client and a cluster of servers includes conducting a first communication session over a network between the client and a selected one of the servers in the cluster, and sending identifying data generated responsive to the first communication session over the network for storage by the client. Upon receiving a request from the client to conduct a second communication session, the request comprising the identifying data, the request is routed to the selected server responsive to the identifying data in the request.

This invention discloses functional humanized antibody against CD20 molecules and its application. The functional humanized antibody specifies human CD20 molecules, and is composed of a light chain and a heavy chain, each of which comprises a constant region and a variable region. The constant region of the heavy chain is isotypic human IgG1 constant region, and that of the light chain is isotypic human kappa constant region. The variable region of the heavy chain is shown in SEQ ID No.2, and that of the light chain is shown in SEQ ID No.1. The functional humanized antibody has low immunogenicity. Experiments show that the functional humanized antibody has the same specificity and affinity as the original murine antibody. Drugs containing the functional humanized antibody and its derivatives can be used for treating cancers or immune system diseases with the high-expression of CD20, such as rheumatic arthritis and lupus erythematosus.

The disclosed construction method for sandwich antibody chip detection system based on single-chain anti-body fused protein comprises: constructing the express carrier for Z186-L-SBP and Z163-L-SBP, designing upper and downstream primer, connecting double-chain ends as NotI and BamHI limited enzyme sites to the product; constructing the express carrier for Z163-Fc, and screening the single-chain antibody fused protein by ELISA method. This invention has wide application.

An input/output (I/O) switch fabric includes first physical ports that convey multiple network flows. Moreover, classifiers in the I/O switch fabric separate packets for network flows associated with different types of service. Then, the I/O switch fabric conveys the packets to different virtual switch ports without interference between the separated packets associated with different network flows. Furthermore, second physical ports in the I/O switch fabric output the packets, where a given second physical port outputs packets for at least some of the network flows associated with different types of service. In this way, the given second physical port can output packets having: the same source and destination; different sources and the same destination; or the same source and different destinations.

Disclosed herein is the use of three-dimensional structure information to guide the process of modifying antibodies with amino acids from one or more templates or surrogates such that the antigen binding properties of the parent antibody are maintained and the immunogenicity potential is reduced when administered as a therapeutic in humans.

A system for maintaining SIP application session affinity, the system including a destination inspector configured to inspect a SIP request to determine whether the SIP request indicates as its destination a logical name of a SIP container, a request router configured to route the SIP request to the SIP container that is identified by the logical name if the SIP request indicates as its destination the logical name of the SIP container, and a destination assignor configured to assign the SIP request to a SIP container in accordance with a predefined assignment protocol if the SIP request does not indicate as its destination the logical name of a SIP container.

The embodiment of the invention provides a video processing method and device, electronic equipment and a storage medium, and relates to the technical field of image processing. The method comprises the following steps: receiving an anchor assistant image and an anchor image sent by an image acquisition device; identifying a real-time expression of the anchor assistant image, and generating a cartoon head portrait of the corresponding expression; replacing the anchor assistant image with the cartoon head portrait. The cartoon head portrait and the anchor image are subjected to video synthesisand output display, interaction with a main teacher is carried out through the cartoon head portrait, the course interestingness is increased, and the problem that the classroom efficiency is influenced due to the fact that the attention of students is difficult to attract in existing online teaching is solved.

The present invention is characterized by a D-Aptamer-Like Peptide (D-Aptide) or retro-inverso Aptide which specifically binds to a target comprising: (a) a structure stabilizing region comprising parallel, antiparallel or parallel and antiparallel D-amino acid strands with interstrand noncovalent bonds; and (b) a target binding region I and a target binding region II comprising randomly selected n and m D-amino acids, respectively, and coupled to both ends of the structure stabilizing region. The D-Aptide or retro-inverso Aptide has the sequence of the same or opposite direction to L-Aptide, wherein the stability to proteases is improved while maintaining the affinity to a target compared with L-Aptide. The D-Aptide of the present invention has substantially the same target affinity and a remarkably improved stability compared with L-Aptide which is different from a general technical knowledge.

The invention relates to a method for preparing photovoltaic silicon blade materials used for line cutting, comprises the following concrete steps of: 1. fine crushing: crushing silicon carbide raw materials; 2. chemical treatment: removing impurities by adopting deionized water and foaming agents; 3. standing treatment; 4. fine grading; 5. drying treatment; and 6 mixing and proportioning, wherein in the mixing and proportioning step, firstly, different batches are sorted after being detected according to particle shapes, rhombic granular materials and ball-shaped granular materials are mixed according to a mass ratio of 1/2, and packing is carried out after the sieving treatment. The invention has simple production process, can not cause any pollution on environment, in addition, the fine powder of the finely processed silicon carbide has high purity and concentrated granularity, the cutting efficiency can be improved, and the service life can be prolonged.

The invention belongs to the technical field of microorganism detection, and discloses a rapid pathogenic microorganism detection method which comprises the following specific steps: preparing a detection probe and a capture probe, carrying out incubating to obtain a sandwich product, drawing a standard working curve, and detecting a to-be-detected sample. The detection probe comprises a nanogold@ nitrogen doped graphene quantum dot nano material, and a sulfydryl-containing silent region Raman signal molecule and a broad-spectrum recognition molecule which are modified on the surface of the nano material; and the capture probe is an immunomagnetic bead modified with an antibody. Raman detection of pathogenic microorganism is realized by combining the detection probe with immunomagnetic beads, namely a mechanism of specific recognition coupled with broad-spectrum recognition. The sensor construction and detection process do not need large instruments, operation is simple and rapid, andthe sensitivity is high.

The present invention relates to a modified L49 single chain antibody (L49-sFv) that exhibits increased refolding efficiency and/or greater stability in mouse serum, and surprisingly substantially maintains binding affinity for its binding ligand, p97 melanotransferrin. p97 melanotransferrin is expressed on the surface of a number of types of cancer (carcinoma) cells, e.g., melanoma cells, lung cancer cells, renal cancer cells, colon cancer cells. The present invention also relates to a modified L49-sFv fused or conjugated to a therapeutic agent, such as a cytotoxic molecule or a pro-drug converting enzyme. The present invention also relates to methods of using the modified L49-sFv molecules fused or conjugated to a therapeutic agent for treatment and/or prophylaxis of cancer, which cancer cells express p97 melanotransferrin.

The invention discloses a preparation method of oral nanoparticles for penetrating gastrointestinal mucus and epithelial cell barriers. The method comprises the following steps: (1) preparing a pH response material: connecting polyethylene glycol to stearic acid through an acylhydrazone bond to obtain the pH response material; (2) preparing a polymer material: connecting chitosan oligosaccharide to stearic acid through an amido bond to obtain the polymer material; and (3) preparing the nanoparticles: mixing the pH response material in the step (1) to the polymer material in the step (2), and performing nano coprecipitation self-assembly to obtain the core-shell oral nanoparticles taking the polymer material as an inner core and the pH response material as an outer shell. According to the nanoparticles prepared by the preparation method provided by the invention, in a mucus microenvironment, the acylhydrazone bond is broken and falls off from a polyethylene glycol shell, and a CSO-SA core is exposed, so that conversion from hydrophilicity to hydrophobicity, conversion from near-neutrality to positive charges and increase of adhesion of the surface of the nanoparticles are realized,the epithelial cell barrier spanning capability of the nanoparticles is enhanced, and the oral absorption rate of drugs is increased.

The invention belongs to the field of food biotechnology, and discloses a fish skin collagen polypeptide and its preparation method and application, comprising the following preparation steps: (1) immobilizing Bacillus subtilis alkaline protease (BAP) with egg shell membrane (ESM) as a carrier , to obtain ESM‑BAP; (2) Utilizing ESM‑BAP to enzymatically hydrolyze fish skin collagen and separate it through a 1-5kDa ultrafiltration membrane, and to screen and verify through human bone marrow mesenchymal stem cell experiments and zebrafish animal experiments, The fish skin collagen polypeptide with high anti-osteoporosis activity is obtained. The raw material of the fish skin collagen polypeptide obtained in the present invention is derived from fish processing waste, has high safety without any side effects, greatly increases the value of fish by-products, and at the same time reduces environmental pollution to a certain extent. The obtained collagen polypeptide is safe and reliable, has high anti-osteoporosis activity, and can be used in various fields such as food and medical treatment.

Provided is a method for preparing a block copolymer including a step of subjecting a lactide monomer to ring-opening polymerization in the presence of a biosynthesized poly(3-hydroxypropionate) initiator to prepare a polylactide-poly(3-hydroxypropionate) block copolymer.

The invention discloses a preparation method for mortar with a low dry shrinkage rate, and relates to the technical field of building materials. The method includes the following steps: (1) preparingactivated starch ether; and (2) preparing the mortar. According to the method, the activated starch ether is added into the mortar, so that the consistency of the mortar can be improved, and at the same time, the construction performance, sagging resistance and shrinkage resistance of the newly stirred mortar can be effectively improved.