A method for extracting and isolating Trametesic acid from Inonotus obliquus
A technology of Inonotus obliquus and Trametes acid, which is applied in the field of biological extraction, can solve the problems of excessive consumption of organic solvents, limited use, cumbersome process, etc., achieves improved purity and yield, is suitable for industrial applications, and shortens separation the effect of time
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Embodiment 1
[0080] S1. Grinding the Inonotus obliquus powder dried to constant weight into a coarse powder with a particle size of 40 mesh for later use. Weigh 660 g of the pulverized Inonotus obliquus in S1, add 660 mL of 50% isopropanol, soak for 0.5 h, and pack into a chromatographic column of 60.6 mm × 374 mm (diameter × height). The flow rate was eluted with 50% isopropanol for 1 h, and 1.08 L of the eluate was collected. The extract was concentrated under reduced pressure at 50°C to 1 / 2 the volume of the eluate, that is, 540 mL; the extraction result of this step was detected by HPLC.
[0081] S2. Add 540 mL of ethyl acetate to extract for 30 minutes, and collect the upper ethyl acetate phase. Extract three times, 30 minutes each. The three ethyl acetate phases were combined for a total of 1.62 L, and the ethyl acetate was recovered under reduced pressure at 60°C to obtain 6.69 g of dry powder; the extraction result of this step was detected by HPLC.
[0082] S3. Put the dry powde...
Embodiment 2
[0093] S1. Grinding the Inonotus obliquus powder dried to constant weight into a coarse powder with a particle size of 40 mesh for later use. Weigh 1320g of Inonotus obliquus powder pulverized in S1, add 1320mL of 50% isopropanol, soak for 0.6h, and then pack into two 60.6mm×374mm (diameter×height) chromatographic columns assembled in series, Elute with 50% isopropanol at a flow rate of 2.16 L / h for 1 h, collect 2.16 L of the eluate, and concentrate the extract under reduced pressure at 50°C to 1 / 2 the volume of the eluate, ie 1080 mL.
[0094] S2. Add 1080 mL of ethyl acetate to extract for 30 minutes, and collect the upper ethyl acetate phase. Extract three times, 30 minutes each. The three ethyl acetate phases were combined for a total of 3.24, and the ethyl acetate was recovered under reduced pressure at 60°C to obtain 13.5 g of dry powder.
[0095] S3. Put the dry powder into the loading column, which is connected to a chromatographic column (column diameter×column bed ...
Embodiment 3
[0098] S1. Grinding the Inonotus obliquus powder dried to constant weight into a coarse powder with a particle size of 40 mesh for later use. Weigh 300 g of the pulverized Inonotus obliquus powder in S1, add 300 mL of 70% isopropanol, soak it for 0.5 h, and pack it into a chromatographic column of 60.6 mm×374 mm (diameter×height), and use 1.08 L / h The flow rate was eluted with 70% isopropanol for 0.5h, and 540mL of the eluate was collected. The extract was concentrated under reduced pressure at 55°C to remove the isopropanol, and 160mL of the concentrated solution was obtained; the extraction result of this step was detected by HPLC.
[0099] S2. Add 160 mL of ethyl acetate to extract for 40 minutes, and collect the upper ethyl acetate phase. Extract three times, 30 minutes each. The three ethyl acetate phases were combined to a total of 480 mL, and the ethyl acetate was recovered under reduced pressure at 58°C to obtain 3.02 g of dry powder; the extraction result of this ste...
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