Method for promoting nerve cell growth and cross-linking under induction of extremely low frequency electromagnetic field

Abstract

The invention discloses a method for promoting nerve cell growth and cross-linking under the induction of an extremely low frequency electromagnetic field. The method comprises the following steps: step 1, an extremely low frequency electromagnetic field generator for stimulating nerve cells is built; step 2, a close-wound solenoid receives a pulse signal sent by a signal generator to generate anelectromagnetic field with a corresponding waveform; step 3, the frequency of the signal sent by the signal generator is 50Hz, and the current is 0.3A; step 4, the amplification factor of a power amplifier is 10; step 5, SH-SY5Y nerve cells are cultured to a logarithmic phase for later use; step 6, the SH-SY5Y nerve cells in the logarithmic phase are divided into a plurality of groups; step 7, theSH-SY5Y nerve cells are stimulated by the extremely low frequency electromagnetic field; and step 8, the activity, proliferation rate and the differentiation degree of the stimulated cells are detected. The method proves the growth promoting effect of the extremely low frequency electromagnetic field on the SH-SY5Y nerve cells, proves the potential treatment effect of the extremely low frequencyelectromagnetic field on a nervous system, and is safer and more convenient.

Description

technical field [0001] The invention relates to the technical fields of neurobiology and electromagnetic biology, in particular to a method for promoting nerve cell growth and crosslinking induced by an extremely low frequency electromagnetic field. Background technique [0002] With the rapid development of modern technology and the continuous improvement of informatization, the use of functional electromagnetic fields (electromagnetic field, EMF) in industrial production and residential life is becoming wider and wider. EMF has become the most common necessity for human life. one of the elements. The continuous development of electromagnetic field functions can expand the application range of electromagnetic waves of different spectrums and expand the application depth. Therefore, the exposure level of electromagnetic waves of different frequencies is gradually increasing, and the related research fields are also expanding. The discovery of new functions and applications...

AI Technical Summary

Problems solved by technology

However, in view of the inconsistency of research objects, parameters used and research conditions, including differences in exposure range, radiation frequency, intensity, time, absorption rate and neurobehavioral detection methods used, the results of existing epidemiological research reports and The conclusions are varied and lack comparability, making it impossible for people to draw more consistent and positive conclusions from existing reports

In the experimental research of neurochemistry and molecular biology, the existing research is relatively in-depth, but in general, the research in the molecular field mostly requires in vitro test analysis, the test methods are cumbersome and complicated, and there are many interference factors, so the existing experimental results are also lacking. Consistency and Repeatability

Therefore, although the existing experimental research reports have involved many aspects of low-field-strength electromagnetic radiation affecting advanced brain functions, including learning, memory, emotion, sleep, etc., is it possible to confirm the existence of such effects? Specifically, which subregion of the brain is mainly affected by electromagnetic radiation of a specific frequency and field strength? What kind of impairment of cognitive processes is ultimately involved? far from elucidated

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