Rice grain length and grain weight related protein as well as coding gene and application thereof

A technology that encodes genes and proteins, applied in applications, genetic engineering, plant genetic improvement, etc., can solve the problems of unclear genetic regulatory pathway network, unknown genetic interaction relationship, increasing grain length and yield, etc.

Active Publication Date: 2021-01-15
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High expression of GLW7 increases grain length and yield by promoting cell elongation, while its loss-of-function mutants produce short grains
In addition, GLW7 can bind to the promoters of SRS5 and DEP1 to promote their expression, but the specific genetic interaction relationship is still unknown
[0005] Although many genes that regulate seed grain shape have been cloned, their genetic regulatory pathway network is still not clear, and the relationship between them needs to be further studied

Method used

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  • Rice grain length and grain weight related protein as well as coding gene and application thereof
  • Rice grain length and grain weight related protein as well as coding gene and application thereof
  • Rice grain length and grain weight related protein as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the acquisition of OsMOB1A protein and its coding gene

[0051] Total RNA was extracted from Zhonghua 11 rice seedlings and reverse transcribed into cDNA. After a large number of sequence analysis, expression analysis and functional verification, a DNA coding sequence was found from the cDNA, as shown in sequence 2 of the sequence listing, and its encoded protein was shown in sequence 1 of the sequence listing.

[0052] The protein shown in Sequence 1 of the Sequence Listing is named OsMOB1A protein. The gene encoding OsMOB1A protein is named OsMOB1A gene, and its open reading frame is shown in sequence 2 of the sequence listing.

Embodiment 2

[0053] Embodiment 2, recombinant expression vector construction and acquisition of recombinant Agrobacterium

[0054] 1. Construction of recombinant expression vector

[0055] The fragment between the BamHI and KpnI restriction sites of the pUN1301 vector was replaced with the DNA molecule shown in Sequence 2 of the Sequence Listing to obtain the recombinant expression vector pUN1301-OsMOB1A (sequencing verification).

[0056] Second, the acquisition of recombinant Agrobacterium

[0057] 1. The recombinant plasmid pUN1301-OsMOB1A was introduced into Agrobacterium EH105 to obtain the recombinant Agrobacterium, which was named recombinant Agrobacterium EH105 / pUN1301-OsMOB1A.

[0058] 2. The pUN1301 vector was introduced into Agrobacterium EH105 to obtain recombinant Agrobacterium, which was named recombinant Agrobacterium EH105 / pUN1301.

Embodiment 3

[0059] Example 3, Functional verification of OsMOB1A protein in rice

[0060] 1. Obtaining of rice plants transgenic for OsMOB1A

[0061] Through the rice genetic transformation system mediated by Agrobacterium, the recombinant Agrobacterium EH105 / pUN1301-OsMOB1A prepared in Example 2 was transferred into the callus of rice (OryzasativaL.cvZhonghua11, hereinafter referred to as wild-type rice) embryo. Induction, pre-cultivation, infection, co-cultivation, selection of hygromycin-resistant calli, differentiation, rooting, transplanting seedlings to obtain transgenic plants. Transgenic OsMOB1A rice plants of the T0 generation were obtained by transformation of 10 lines. Specific steps are as follows:

[0062] 1) Induction and subculture of rice mature embryogenic callus: select wild-type Zhonghua No. 11 rice seeds with plump grains and remove glumes, and add 70% ethanol for surface disinfection for 5 minutes. Then disinfect it with 20% sodium hypochlorite (NaClO) for 20 minut...

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Abstract

The invention discloses a rice grain length and grain weight related protein as well as a coding gene and application thereof. According to the invention, an OsMOB1A gene is introduced into wild typerice by using a recombinant vector to obtain transgenic rice. Compared with the wild type rice, for the transgenic rice, the OsMOB1A gene is overexpressed, the grain length of the rice is obviously increased, and the grain weight is obviously increased. Therefore, the OsMOB1A gene is related to the grain shape, and a foundation is laid for improving the cultivation of transgenic plants with changed grain shapes. The invention has important theoretical significance and practical significance for further clarification of a molecular mechanism of plant grain development and cultivation of high-quality and high-yield new crop varieties through genetic engineering means.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a protein related to rice grain length and grain weight, its coding gene and its application. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population uses rice as a staple food. In recent years, with the increasingly serious problems of decreasing arable land, energy shortage, climate change and increasing world population, the problem of food security has become more and more serious. Therefore, increasing rice yield and improving rice quality has become an important research goal of plant breeding in my country. The three major elements of rice yield: the number of panicles per plant, the number of grains per panicle, and the weight of grains. The grain shape traits of rice include grain length, grain width, grain thickness and grain aspect ratio, which are positively correlated wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00A01H6/46C07K14/415C12N15/29
CPCC12N15/8205C12N15/8261C07K14/415
Inventor 程佑发周芳芳凌艳霞
Owner INST OF BOTANY CHINESE ACAD OF SCI
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