Method for separating and purifying stable-ploidy regeneration plants from polyploidy dioscorea alata

A technology for separating, purifying and regenerating plants, which is applied in the fields of botanical equipment and methods, plant regeneration, and horticultural methods. effect of time and enrichment of germplasm resources

Pending Publication Date: 2021-01-22
三明市农业科学研究院
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Problems solved by technology

[0004] The present invention provides a method for separating and purifying ploidy-stable regenerated plants from mixed ploidy ginseng. Defects such as promotion and use as breeding

Method used

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  • Method for separating and purifying stable-ploidy regeneration plants from polyploidy dioscorea alata
  • Method for separating and purifying stable-ploidy regeneration plants from polyploidy dioscorea alata
  • Method for separating and purifying stable-ploidy regeneration plants from polyploidy dioscorea alata

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Embodiment Construction

[0031] Reference manual attached Figure 1-15 . A method for separating and purifying ploidy-stable regenerated plants from mixed ploidy ginseng, comprising the following steps:

[0032] a) Sterilization of stems: In mid-June, cut the mixed ploidy ginseng stems of the same plant into about 1cm stems with axillary buds, rinse them with water, put them in bottles, rinse them with running tap water for 1-2 hours, and wait After drying, soak it in 75% alcohol for 15 s on the ultra-clean workbench, rinse it with sterile water for 2-4 times, then put it into 0.1% mercuric chloride, shake it continuously, and take it out after 8-10 min. Rinse with sterile water for 5-8 times before use;

[0033] b) Callus induction: Inoculate the sterilized stem segments into the callus induction medium for induction treatment. The callus induction medium is: 1 / 2MS+2,4-D 0.5 mg / L+6-BA 2.0 mg / L+NAA 0.2 mg / L+coconut juice 70 mL / L.

[0034] c) Differentiation culture: transfer the induced callus (wi...

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Abstract

The invention relates to a method for separating and purifying stable-ploidy regeneration plants from polyploidy dioscorea alata. The method mainly comprises the following steps of (1) sterilizing stems; (2) inducing calluses; (3) carrying out differentiation culture; (4) carrying out rooting culture; (5) carrying out root tip chromosome number identification; and (6) carrying out biological character and chromosome ploidy stability re-identification. The method is ideal in design, tissue culture is carried out on the polyploid (such as tetraploid and hexaploid) dioscorea alata stems serving as explants, the regeneration plants with single ploidy and stable biological characters can be obtained, strains with different chromosome ploidy and biological characters can also be obtained, and the polyploidy dioscorea alata can be used in a breeding plan. The germplasm resources of the dioscorea alata are enriched, the breeding time is shortened, a new way is opened up for dioscorea alata breeding, and a foundation is laid for later variety breeding.

Description

technical field [0001] The invention relates to the separation and purification technology of mixed ploidy plants, in particular to a method for separating and purifying ploidy stable regenerated plants from mixed ploidy ginseng. Background technique [0002] Ginseng potato, also known as big potato, belongs to Dioscoreaceae and belongs to perennial rattan herb. Its tubers are rich in starch and crude protein, which can be used as food and vegetables, and can be used for starch production and medicinal purposes. In China, it is mainly produced in Zhejiang, Guangdong, Guangxi, Hunan, Hubei, Fujian, Sichuan, Jiangxi and other provinces. In addition to eating, ginseng is also a medicinal plant. According to "Shen Nong's Materia Medica" and "Compendium of Materia Medica", ginseng has the effects of strengthening the spleen and stomach, promoting body fluid and lungs, nourishing kidney and essence, and benefiting the brain and beauty. Aging, treatment of diabetes, cardiovascular...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 周建金华树妹叶炜李丽红陈芝华邓才生
Owner 三明市农业科学研究院
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