Indian kalimeris herb extract and preparation method and application thereof
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A technology of extract and Malan, applied in the field of Malan extract and its preparation, can solve the problems of low extraction rate of flavonoids, difficulty in guaranteeing the stability of the extract, residual organic solvents, etc.
Pending Publication Date: 2021-01-22
BEIJING ACAD OF TCM BEAUTY SUPPLEMENTS
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The above extraction method has problems such as low extraction rate of flavonoids, residual organic solvent, difficulty in guaranteeing the stability of the extract, etc., and the obtained extract is not suitable for use in skin care products
[0004] At present, Malan extract has not been widely used in skin care products. To develop a preparation method with high extraction rate and good stability of Malan active ingredients, and to apply the obtained Malan extract to the field of skin care products, the in-depth development and wide application of Malan application is important
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Embodiment 1
[0124] (1) Dry the whole Malan herb to constant weight, crush, pass through a 40-60 mesh sieve (take the part under the 40-mesh sieve and the part above the 60-mesh sieve for use), and add a certain amount of water;
[0125] (2) Ultrasonic treatment of the Malan solution obtained in step (1) at a temperature of 60°C to obtain Malan ultrasonic suspension;
[0126] (3) Adjust the pH value of the Malan ultrasonic suspension in step (2), add pepsin for initial enzymatic hydrolysis to obtain Malan enzymatic hydrolysis solution Ⅰ;
[0127] (4) Adjusting the pH value of step (3) Malan enzymatic hydrolysis solution Ⅰ, adding cellulase for secondary enzymolysis to obtain Malan extract;
[0128] (5) Inactivate the enzyme at 90-95°C for 30 minutes, and filter to obtain Malan extract.
[0129] Specific solid-liquid ratio (m / m), enzyme addition amount (%), enzymatic hydrolysis temperature (°C), enzymolysis time (min), ultrasonic power (W), ultrasonic extraction time of sample 1-4 ultrason...
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Abstract
The invention relates to an Indian kalimeris herb extract and a preparation method and application thereof. The preparation method of the Indian kalimeris herb extract comprises the following steps that (1), Indian kalimeris herb is dried, smashed and screened, and a certain amount of water is added; (2), ultrasonic treatment is conducted on an Indian kalimeris herb solution obtained in the step (1), and an Indian kalimeris herb ultrasonic suspension is obtained; (3), primary enzymolysis is conducted, the pH value of the Indian kalimeris herb ultrasonic suspension obtained in the step (2) is adjusted, pepsin is added for primary enzymolysis, and an Indian kalimeris herb enzymatic hydrolysate I is obtained; and (4), the pH value of the Indian kalimeris herb enzymatic hydrolysate I obtainedin the step (3) is adjusted, cellulase is added for secondary enzymolysis, and the Indian kalimeris herb extract is obtained. The preparation method of the Indian kalimeris herb extract is simple, andthe prepared Indian kalimeris herb extract is high in flavone content and good in stability. Meanwhile, the Indian kalimeris herb extract can significantly reduce skin irritation reactions caused bylactic acid, has the effects of tightening skin, replenishing water and preserving moisture and can be widely applied to skin care products or cosmetics.
Description
technical field [0001] The invention relates to the field of plant extraction research, in particular to a Malan extract and its preparation method and application. Background technique [0002] Malan is a perennial herb of the genus Malan in the family Asteraceae. It is cool in nature and pungent in taste, and it belongs to the lung, liver, stomach, and large intestine meridians. The active ingredients of Malan are located in leaves, stems, and roots, and mainly contain flavonoids, polyphenols, polysaccharides, volatile oils, and alkaloids. Literature (Deng Xi, Wang Lin, Yang Haitao. Extraction of total flavonoids from Malan and its antioxidant activity[J]. Hubei Agricultural Science, 2016, 55(16): 4241-4245+4249.) disclosed Malan flavonoids It has anti-oxidation, free radical scavenging, anti-aging, anti-cancer, anti-virus and other effects; at the same time, literature (Zhu Xiaolin, Liu Yuejun, Lu Min, Lu Lishuang. The active components of different varieties of Malan an...
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