Kit for determining calprotectin by latex immunoturbidimetry

A technology of calprotectin and immunoturbidity, which is applied in the field of calprotectin kits, can solve the problems of large deviation of test results and insufficient stability, and achieve high stability and high accuracy

Inactive Publication Date: 2021-01-26
SHENZHEN HONGMED INFAGEN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above kits have the advantages of being stable, accurate, time-saving and labor-saving, they have t

Method used

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  • Kit for determining calprotectin by latex immunoturbidimetry
  • Kit for determining calprotectin by latex immunoturbidimetry
  • Kit for determining calprotectin by latex immunoturbidimetry

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of kit

[0030] A test kit for measuring calprotectin by latex immunoturbidimetric method, comprising R1 reagent, R2 reagent and calprotectin antigen calibrator solution, the test kit includes a sealable box body and a reagent fixture placed in the box body, Wherein the reagent fixing part 2 is a paper fixing part or a polymer material fixing part, and the reagent fixing part is provided with a cavity, including a cavity one and a cavity two, and the cavity one is equipped with a reagent bottle R1, and the cavity two Contains reagent bottle R2.

[0031] R1 reagent: made up of the following components by weight percentage: 3% sodium chloride, 2.5% sorbitol, 5% Tween 80, 0.5% sodium azide, pH7.4, 250mmol / L MES buffer liquid.

[0032] R2 reagent: made of the following components by weight percentage: 4mg / mL latex particles coated with anti-human calprotectin polyclonal antibody, 3% sodium chloride, 2.5% sorbitol, 5% Tween 80 , 0.5% sodium a...

Embodiment 2

[0035] The sorbitol in the R1 reagent of Example 1 was replaced by PEG-600 of the same weight, and the sorbitol in the R2 reagent was also replaced by the PEG-600 of the same weight.

Embodiment 3

[0037] Only the sorbitol in the R2 reagent of Example 1 was replaced with PEG-600 of the same weight.

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Abstract

The invention relates to a kit for determining calprotectin by latex immunoturbidimetry. The kit comprises a reagent R1 and a reagent R2, wherein the R1 reagent comprises an electrolyte, a stabilizer,a surfactant, a preservative and a buffer solution; the R2 reagent comprises latex particles coated with an anti-human calprotectin polyclonal antibody, an electrolyte, a stabilizer, a surfactant, apreservative and a buffer solution; the average particle size of the latex particles ranges from 50 nm to 500 nm; and the stabilizing agent of the reagent R1 is selected from sorbitol and/or PEG (polyethylene glycol), and the stabilizing agent of the reagent R2 is selected from sorbitol and/or PEG (polyethylene glycol). The kit for determining calprotectin by latex enhanced immunoturbidimetry hashigher stability, and can still maintain higher accuracy under the condition of long-term storage.

Description

technical field [0001] The invention relates to the fields of medical testing and in vitro diagnosis, in particular to a kit for measuring calprotectin in human blood or feces excretion by using latex immune turbidimetry. Background technique [0002] Calprotectin is a calcium and zinc binding protein with a molecular weight of 36kD, which is composed of two heavy chains with a molecular weight of 14kD and a light chain with a molecular weight of 8kD. It can bind two Ca ions, so it has the characteristics of heat resistance and enhanced hydrolysis. Its protein structure is a complex composed of two subunits S-100A8 and S-100A9. It is distributed in granulocytes, monocytes, keratinocytes and various tissues and body fluids of the human body, and is a protective protein. [0003] Calprotectin is an acute inflammatory marker derived from neutrophils and macrophages. Its expression is tissue- or cell-specific. It is a heterozygous calcium-binding protein that has anti-protease ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/68
CPCG01N21/31G01N33/68
Inventor 侯志波巴里·马歇尔郑敬元张伟
Owner SHENZHEN HONGMED INFAGEN CO LTD
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