Nucleic acid detection device

Abstract

The invention discloses a nucleic acid detection device, and relates to the technical field of nucleic acid detection. The nucleic acid detection device comprises a nucleic acid extraction device andsplitter plates, wherein an interface communicated with the inside of the nucleic acid extraction device is arranged on the nucleic acid extraction device, a power chamber is arranged on each splitterplate, the interface and the power chambers are connected through conduits, a connecting piece and an annular buckle, and the connecting piece is fixedly connected with the conduits. The annular buckle is fixedly connected to the power chamber, a channel communicating with the power chamber is formed in the annular buckle, and a groove matched with the annular buckle is formed in the connecting piece. Compared with the prior art, the technical scheme provided by the invention has the beneficial effects that (1) the working efficiency is improved, and the result stability is high; (2) the detection requirement is met, the safety is high, and the laboratory pollution risk is reduced; and (3) the precision is high, and higher requirements are met.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid detection device. Background technique [0002] The most common method for detecting nucleic acid is fluorescent quantitative PCR (polymerase chain reaction). Since the PCR reaction template is only DNA, nucleic acid (RNA) should be reverse transcribed into DNA before performing the PCR reaction. In the PCR reaction system, a pair of specific primers and a Taqman probe are included. The probe is a specific oligonucleotide sequence, and the two ends are respectively labeled with a reporter fluorescent group and a quencher fluorescent group. When the probe is intact, the fluorescent signal emitted by the reporter group is absorbed by the quencher group; if there is a target sequence in the reaction system, the probe binds to the template during the PCR reaction, and the DNA polymerase uses the exonuclease activity of the enzyme to bind the probe along...

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Problems solved by technology

[0004] The main disadvantages are: the current existing nucleic acid detection mainly has the following problems: (1) In the face of a large number and complex detection steps, manual operations are prone to errors, and the overall operation steps are complicated, making it impossible to perform efficient and fast target nucleic acid detection. (2) Most molecular diagnosis needs to be carried out in the laboratory. Many grassroots units do not have the conditions to establish a standard molecular diagnosis laboratory. In addition, the operating habits and proficiency of the operators are different. prone to cross-contamination of samples

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